Transgene vaccination using Ulex europaeus agglutinin I (UEA-1) for targeted mucosal immunization against HIV-1 envelope

Vaccine ◽  
2005 ◽  
Vol 23 (29) ◽  
pp. 3836-3842 ◽  
Author(s):  
Xinhai Wang ◽  
Irina Kochetkova ◽  
Asmahan Haddad ◽  
Teri Hoyt ◽  
David M. Hone ◽  
...  
Keyword(s):  
Mucosal Immunization ◽  
Ulex Europaeus ◽  
Ulex Europaeus Agglutinin I ◽  
Hiv 1

scholarly journals Fabrication of Carbohydrate Chips Based on Polydopamine for Real-Time Determination of Carbohydrate–Lectin Interactions by QCM Biosensor

Polymers ◽  
2018 ◽  
Vol 10 (11) ◽  
pp. 1275 ◽  
Author(s):  
Kun Shang ◽  
Siyu Song ◽  
Yaping Cheng ◽  
Lili Guo ◽  
Yuxin Pei ◽  
...  
Keyword(s):  
Real Time ◽  
Con A ◽  
Quartz Crystals ◽  
Ulex Europaeus ◽  
Novel Approach ◽  
Ulex Europaeus Agglutinin I ◽  
Flow Through ◽  
Time Determination ◽  
Kinetics Of

A novel approach for preparing carbohydrate chips based on polydopamine (PDA) surface to study carbohydrate–lectin interactions by quartz crystal microbalance (QCM) biosensor instrument has been developed. The amino-carbohydrates were immobilized on PDA-coated quartz crystals via Schiff base reaction and/or Michael addition reaction. The resulting carbohydrate-chips were applied to QCM biosensor instrument with flow-through system for real-time detection of lectin–carbohydrate interactions. A series of plant lectins, including wheat germ agglutinin (WGA), concanavalin A (Con A), Ulex europaeus agglutinin I (UEA-I), soybean agglutinin (SBA), and peanut agglutinin (PNA), were evaluated for the binding to different kinds of carbohydrate chips. Clearly, the results show that the predicted lectin selectively binds to the carbohydrates, which demonstrates the applicability of the approach. Furthermore, the kinetics of the interactions between Con A and mannose, WGA and N-Acetylglucosamine were studied, respectively. This study provides an efficient approach to preparing carbohydrate chips based on PDA for the lectin–carbohydrate interactions study.

Abstract 1945: Levels Of Circulating Progenitor Cells In Patients With Chronic Heart Failure Predict Outcomes

Circulation ◽  
2007 ◽  
Vol 116 (suppl_16) ◽  
Author(s):  
Roberto Latini ◽  
Ralph Lehmann ◽  
Birgit Assmus ◽  
Giovanna Balconi ◽  
Stefanie Dimmeler ◽  
...  
Keyword(s):  
Heart Failure ◽  
Progenitor Cells ◽  
Mononuclear Cells ◽  
Nyha Class ◽  
Double Positive ◽  
Ulex Europaeus ◽  
Vascular Regeneration ◽  
Circulating Endothelial Progenitor Cells ◽  
Cellular Marker ◽  
Ulex Europaeus Agglutinin I

Background: A low level of circulating endothelial progenitor cells (EPCs), a marker of reduced vascular regeneration, is related to worse outcome in patients with coronary heart disease, but little is known on pts with heart failure (HF). The aim was to assess the prognostic value of circulating EPCs in patients with HF. Methods: In 68 pts enrolled to GISSI-HF trial by 5 Italian Centers and 45 pts from Frankfurt, mononuclear cells from peripheral blood were isolated and plated; after 4 days, adherent cells were incubated with DiI-labeled acetylated LDL, stained with FITC-labeled Ulex europaeus agglutinin I (lectin), and counted as double positive at confocal microscopy. 66 patients had ischemic etiology, 12 were females, 26 had NYHA class >II, median age 64 y (Q1–Q3 56–70y). Cutoffs for EPCs were assessed by ROC. Results: Mean EPCs in pts with HF 34 ± 7 EPCs/mm 2 , 2–3 fold lower than in age-matched controls. Over a median of 2.5 years, 12 CV deaths and 47 first hospitalizations for CV reasons were recorded. Levels of EPCs were unrelated to severity of HF, age or sex. After adjustment for clinical variables, creatinine and BNP, EPCs <30/mm 2 were associated with a 2-fold higher risk of CV death and hospitalization, as shown by K-M curves (figure ) and by Cox multivariate regression. Pts with HF of ischemic etiology gave identical results. Conclusions : The level of circulating EPCs is an independent predictor of CV death and hospitalization in pts with chronic HF. The assay of this cellular marker of risk can be performed in blood samples collected in a multicenter setting under controlled conditions, and may offer an accessible tool to assess the role of vascular regeneration in the treatment of HF.

Sarcomatoid Collecting Duct Carcinoma

1999 ◽  
Vol 123 (4) ◽  
pp. 338-341 ◽  
Author(s):  
Masaharu Fukunaga
Keyword(s):  
Collecting Duct ◽  
Fatal Case ◽  
Flow Cytometric Analysis ◽  
Cytokeratin 19 ◽  
Duct Carcinoma ◽  
Collecting Duct Carcinoma ◽  
Ulex Europaeus ◽  
Atypical Cells ◽  
Ulex Europaeus Agglutinin I ◽  
Desmoplastic Stroma

Abstract Background.—Collecting duct carcinoma has recently been recognized as a distinct entity among renal cell carcinomas and shows aggressive clinical behavior. Design.—A fatal case of collecting duct carcinoma with sarcomatoid features in an 80-year-old man is described, and the results of immunohistochemistry and flow cytometric analysis are presented. Results.—The tumor, which was white and had infiltrating margins in the cortex and medulla, consisted of adenocarcinomatous and sarcomatous components. The adenocarcinomatous component was characterized by tubular arrangement of atypical cells with high-grade nuclear features and desmoplastic stroma. The sarcomatous component had a malignant fibrous histocytoma-like appearance. The tumor cells in the adenocarcinomatous area were positive for cytokeratin 19, Ulex europaeus agglutinin I, peanut agglutinin, and soybean agglutinin. The tumor was DNA diploid. The patient died of disease 3 months after diagnosis. Conclusion.—This study suggests that sarcomatoid collecting duct carcinoma may be one of the most aggressive tumors of the kidney.

scholarly journals A Universal Photochemical Method to Prepare Carbohydrate Sensors Based on Perfluorophenylazide Modified Polydopamine for Study of Carbohydrate-Lectin Interactions by QCM Biosensor

Polymers ◽  
2019 ◽  
Vol 11 (6) ◽  
pp. 1023
Author(s):  
Lili Guo ◽  
Shuang Chao ◽  
Pei Huang ◽  
Xiukai Lv ◽  
Quanquan Song ◽  
...  
Keyword(s):  
Schiff Base ◽  
Quartz Crystal Microbalance ◽  
Concanavalin A ◽  
Quartz Crystal ◽  
Sensor Surface ◽  
Con A ◽  
Ulex Europaeus ◽  
Plant Lectins ◽  
Photochemical Method ◽  
Ulex Europaeus Agglutinin I

A universal photochemical method to prepare carbohydrate sensors based on perfluorophenylazide (PFPA) modified polydopamine (PDA) for the study of carbohydrate–lectin interactions by a quartz crystal microbalance (QCM) biosensor was developed. The PFPA was immobilized on PDA-coated gold sensors via Schiff base reactions. Upon light irradiation, the underivatized carbohydrates were inserted into the sensor surface, including mannose, galactose, fucose and N-acetylglucosamine (GlcNAc). Carbohydrate sensors were evaluated for the binding to a series of plant lectins. A kinetic study of the interactions between mannose and concanavalin A (Con A), fucose and Ulex europaeus agglutinin I (UEA-I) were performed. This method can eliminate the tedious modification of carbohydrates, improve the experimental efficiency, and reduce the experimental cost, which is of great significance for the development of QCM biosensors and the study of biomolecular interactions.

scholarly journals Application of Ulex europaeus Agglutinin I-Modified Liposomes for Oral Vaccine: Ex Vivo Bioadhesion and in Vivo Immunity

2011 ◽  
Vol 59 (5) ◽  
pp. 618-623 ◽  
Author(s):  
KeXin Li ◽  
XiuLi Zhao ◽  
ShiYi Xu ◽  
DaHai Pang ◽  
ChunRong Yang ◽  
...  
Keyword(s):  
Ex Vivo ◽  
Oral Vaccine ◽  
Ulex Europaeus ◽  
Ulex Europaeus Agglutinin I

scholarly journals Mucosal Immunization of Lactating Female Rhesus Monkeys with a Transmitted/Founder HIV-1 Envelope Induces Strong Env-Specific IgA Antibody Responses in Breast Milk

2013 ◽  
Vol 87 (12) ◽  
pp. 6986-6999 ◽  
Author(s):  
G. G. A. Fouda ◽  
J. D. Amos ◽  
A. B. Wilks ◽  
J. Pollara ◽  
C. A. Ray ◽  
...  
Keyword(s):  
Breast Milk ◽  
Rhesus Monkeys ◽  
Antibody Responses ◽  
Mucosal Immunization ◽  
Female Rhesus ◽  
Iga Antibody ◽  
Hiv 1

scholarly journals Induction of a Mucosal Cytotoxic T-Lymphocyte Response by Intrarectal Immunization with a Replication-Deficient Recombinant Vaccinia Virus Expressing Human Immunodeficiency Virus 89.6 Envelope Protein

1998 ◽  
Vol 72 (10) ◽  
pp. 8264-8272 ◽  
Author(s):  
Igor M. Belyakov ◽  
Linda S. Wyatt ◽  
Jeffrey D. Ahlers ◽  
Patricia Earl ◽  
C. David Pendleton ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
Vaccinia Virus ◽  
T Lymphocyte ◽  
Envelope Protein ◽  
Cytotoxic T Lymphocyte ◽  
Recombinant Vaccinia Virus ◽  
Mucosal Immunization ◽  
Recombinant Vaccinia ◽  
Immunodeficiency Virus ◽  
Hiv 1

ABSTRACT To improve the safety of recombinant vaccinia virus vaccines, modified vaccinia virus Ankara (MVA) has been employed, because it has a replication defect in most mammalian cells. Here we apply MVA to human immunodeficiency virus type 1 (HIV-1) vaccine development by incorporating the envelope protein gp160 of HIV-1 primary isolate strain 89.6 (MVA 89.6) and use it to induce mucosal cytotoxic-T-lymphocyte (CTL) immunity. In initial studies to define a dominant CTL epitope for HIV-1 89.6 gp160, we mapped the epitope to a sequence, IGPGRAFYAR (from the V3 loop), homologous to that recognized by HIV MN loop-specific CTL and showed that HIV-1 MN-specific CTLs cross-reactively recognize the corresponding epitope from strain 89.6 presented by H-2Dd. Having defined the CTL specificity, we immunized BALB/c mice intrarectally with recombinant MVA 89.6. A single mucosal immunization with MVA 89.6 was able to elicit long-lasting antigen-specific mucosal (Peyer’s patch and lamina propria) and systemic (spleen) CTL responses as effective as or more effective than those of a replication-competent vaccinia virus expressing 89.6 gp160. Immunization with MVA 89.6 led to (i) the loading of antigen-presenting cells in vivo, as measured by the ex vivo active presentation of the P18-89.6 peptide to an antigen-specific CTL line, and (ii) the significant production of the proinflammatory cytokines (interleukin-6 and tumor necrosis factor alpha) in the mucosal sites. These results indicate that nonreplicating recombinant MVA may be at least as effective for mucosal immunization as replicating recombinant vaccinia virus.

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