Sustained exposure to a glycine receptor partial agonist differentially alters NMDA receptor agonist and antagonist potencies in cultured spinal cord neurons

1998 ◽  
Vol 356 (2-3) ◽  
pp. 255-260 ◽  
Author(s):  
Yu Lin ◽  
Linda H. Fossom ◽  
Phil Skolnick ◽  
Joseph B. Long
Keyword(s):  
Spinal Cord ◽  
Nmda Receptor ◽  
Receptor Agonist ◽  
Partial Agonist ◽  
Glycine Receptor ◽  
Spinal Cord Neurons ◽  
Agonist And Antagonist

The influence of NMDA receptor agonist and antagonist on morphine state-dependent memory of passive avoidance in mice

Life Sciences ◽  
2005 ◽  
Vol 78 (2) ◽  
pp. 157-163 ◽  
Author(s):  
Majid Jafari-Sabet ◽  
Mohammad-Reza Zarrindast ◽  
Mehdi Rezayat ◽  
Ameneh Rezayof ◽  
Bijan Djahanguiri
Keyword(s):  
Nmda Receptor ◽  
Passive Avoidance ◽  
Receptor Agonist ◽  
State Dependent ◽  
Agonist And Antagonist

scholarly journals Chemokine CXCL1 enhances inflammatory pain and increases NMDA receptor activity and COX-2 expression in spinal cord neurons via activation of CXCR2

2014 ◽  
Vol 261 ◽  
pp. 328-336 ◽  
Author(s):  
De-Li Cao ◽  
Zhi-Jun Zhang ◽  
Rou-Gang Xie ◽  
Bao-Chun Jiang ◽  
Ru-Rong Ji ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Nmda Receptor ◽  
Inflammatory Pain ◽  
Receptor Activity ◽  
Spinal Cord Neurons ◽  
Cox 2

scholarly journals Glycine Receptor in Rat Hippocampal and Spinal Cord Neurons as a Molecular Target for Rapid Actions of 17-β-Estradiol

2009 ◽  
Vol 5 ◽  
pp. 1744-8069-5-2 ◽  
Author(s):  
Peng Jiang ◽  
Yan Kong ◽  
Xiao-Bing Zhang ◽  
Wei Wang ◽  
Chun-Feng Liu ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Glycine Receptor ◽  
Molecular Target ◽  
Spinal Cord Neurons

Regulation of synapse density by 5-HT2A receptor agonist and antagonist in the spinal cord of chicken embryo

1995 ◽  
Vol 195 (3) ◽  
pp. 159-162 ◽  
Author(s):  
Yoichi Niitsu ◽  
Shun Hatnada ◽  
Kayoko Hamaguchi ◽  
Masahiko Mikuni ◽  
Nobuo Okado
Keyword(s):  
Spinal Cord ◽  
Chicken Embryo ◽  
Receptor Agonist ◽  
Synapse Density ◽  
Agonist And Antagonist

scholarly journals Glycine receptors are not directly modulated by glutamate, AP5 or NMDA

2020 ◽  
Author(s):  
Karin Aubrey ◽  
Diba Sheipouri ◽  
Robert Vandenberg ◽  
Yo Otsu
Keyword(s):  
Spinal Cord ◽  
Drug Exposure ◽  
Glycine Receptor ◽  
Glutamate Transporter ◽  
Inhibitory Synapses ◽  
Future Research ◽  
Published Data ◽  
Glycine Receptors ◽  
Extracellular Glutamate ◽  
Spinal Cord Neurons

3.AbstractReproducibility of research data is a significant problem with more than 60% of biological and medical researchers reporting they have failed to reproduce published data. General acceptance of incorrect results can mean that future data is incorrectly interpreted and progress significantly interrupted. Thus, replication studies play an essential role in corroborating research findings and validating future research objectives. Here, we attempted to replicate data demonstrating the neurotransmitter glutamate, as well as NMDA and AP5, acts as positive allosteric modulators of the inhibitory glycine receptor. Notably, it was shown that the amplitude of miniature glycinergic currents recorded in spinal cord slices were reversibly enhanced when extracellular glutamate concentrations were increased by the glutamate transporter antagonist TBOA. This finding indicates that endogenous fluctuations in extracellular [glutamate] permits cross-talk between excitatory and inhibitory synapses and likely plays a role in setting the spinal inhibitory glycinergic tone and modulating baseline neurotransmission. We re-evaluated the data in primary cultured spinal cord neurons, spinal cord slice and Xenopus laevis oocytes expressing recombinant glycine receptors. Despite extensive efforts, we were unable to reproduce the finding that glutamate, AP5 or NMDA positively modulate glycine receptor currents. We paid careful attention to key aspects of the original study design, ensured rapid drug exposure by using fast-flow application and took into account receptor saturation and protocol deviations such as animal species. This study refutes the finding that glycine receptors are directly modulated by glutamate spill-over and suggests that glycinergic tone is independent of changes in excitatory activity.4.Significance StatementGlutamate spill-over onto inhibitory synapses has been reported to positively modulate glycine receptors and alter the inhibitory tone of the spinal cord. This finding has important implications for baseline spinal transmission and could play a role when chronic pain develops. However, we failed to replicate these results and did not observe any modulation of native or recombinant glycine receptor-mediated currents by AP5, NMDA or glutamate. This indicates that inhibitory glycine receptors operate independently of fluctuations in extracellular [glutamate].5.Visual AbstractN/A

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