Highly sensitive assay for γ-glutamyltranspeptidase activity by high-performance liquid chromatography with electrochemical detection

1986 ◽  
Vol 357 ◽  
pp. 191-198 ◽  
Author(s):  
Kazutoshi Kiuchi ◽  
Kiyomi Kiuchi ◽  
Toshiharu Nagatsu ◽  
Akifumi Togari ◽  
Hidehiko Kumagai
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Electrochemical Detection ◽  
High Performance ◽  
Sensitive Assay ◽  
Highly Sensitive

scholarly journals A SENSITIVE ASSAY FOR NON-SPECIFIC N-METHYLTRANSFERASE ACTIVITY IN RAT TISSUES BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY ELECTROCHEMICAL DETECTION

2017 ◽  
Vol 19 (1) ◽  
pp. 63-68
Author(s):  
Dawan Shimbhu ◽  
Kohichi Kojima ◽  
Toshiharu Nagatsu
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Electrochemical Detection ◽  
High Performance ◽  
Brain Regions ◽  
Assay Method ◽  
Sensitive Assay ◽  
Methyltransferase Activity ◽  
Peripheral Tissues ◽  
Highly Sensitive

 Phenylethanolamine N-methyltransferase (PNMT) and non-specific N -methyltransferase  (EC 2.1.1.28) catalyze the N-methylation of aromatic amines. PNMT is specific for phenylethanolamines such as noradrenaline (NA). and catalyzes the step in catecholamine biosynthesis, forming adrenaline (AD) from NA. PNMT activity is high in adrenal gland, whereas non-specific N-methyltransferase is distributed in various tissues such as the lungs. Borchardt et al. first reported a method to detect PNMT activity by  high-performance liquid chromatography electrochemical detection (HPLC-EICD), which could demonstrate the activity only in the adrenal medulla and hypothalamus. Recently, Troeewicz et al. reported a highly sensitive assay method for PNMT using HPLC-EICD by which the activity in all regions of rat brains could be measured. The activity of non-specific N-methyltransferase in brain regions and peripheral tissues of the rat could be detected by a radioassay. However, there has been no repot on an assay method for non-specific N-methyltransferase using HPLC-EICD. In this paper, we describe a highly sensitive assay procedure for the activity of non-specific N-methyltransferase by high-performance reversed-phase ion pair chromatography with electrochemical detection. By this method, the non-specific N-methyltransferase activity could be determined in various rat brain regions and peripheral tissues.

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