Fatty acid methyl ester (FAME) analysis for monitoring Nocardia levels in activated sludge

1999 ◽  
Vol 33 (8) ◽  
pp. 1964-1966 ◽  
Author(s):  
Daniel K Cha ◽  
Jeffry J Fuhrmann ◽  
Dong W Kim ◽  
Caroline M Golt
Keyword(s):  
Fatty Acid ◽  
Methyl Ester ◽  
Activated Sludge ◽  
Fatty Acid Methyl Ester ◽  
Acid Methyl Ester ◽  
Fame Analysis ◽  
Acid Methyl

FATTY ACID METHYL ESTER (FAME) ANALYSIS FOR MONITORING NOCARDIA LEVELS IN ACTIVATED SLUDGE

2001 ◽  
Vol 2001 (15) ◽  
pp. 780-788
Author(s):  
Il-Kyu Kim ◽  
Daniel K. Cha ◽  
Jeffry J. Fuhrmann ◽  
Ik-Tae Yeom ◽  
Kyu-Hong Ahn
Keyword(s):  
Fatty Acid ◽  
Methyl Ester ◽  
Activated Sludge ◽  
Fatty Acid Methyl Ester ◽  
Acid Methyl Ester ◽  
Fame Analysis ◽  
Acid Methyl

Differentiation between Candida dubliniensis andCandida albicans by Fatty Acid Methyl Ester Analysis Using Gas-Liquid Chromatography

2000 ◽  
Vol 38 (10) ◽  
pp. 3696-3704 ◽  
Author(s):  
Heidrun Peltroche-Llacsahuanga ◽  
Silke Schmidt ◽  
Michael Seibold ◽  
Rudolf Lütticken ◽  
Gerhard Haase
Keyword(s):  
Liquid Chromatography ◽  
Fatty Acid ◽  
Methyl Ester ◽  
Fatty Acid Methyl Ester ◽  
Acid Methyl Ester ◽  
Candida Dubliniensis ◽  
Identification System ◽  
Gas Liquid Chromatography ◽  
Fame Analysis ◽  
Acid Methyl

Candida dubliniensis is often found in mixed culture with C. albicans, but its recognition is hampered as the color of its colonies in primary culture on CHROMagar Candida varies. Furthermore, definite identification of C. dubliniensis is difficult to achieve, time-consuming, and expensive. Therefore, a method to discriminate between these two closely related yeast species by fatty acid methyl ester (FAME) analysis using gas-liquid chromatography (Sherlock Microbial Identification System [MIS]; MIDI, Inc., Newark, Del.) was developed. Although the chromatograms of these two species revealed no obvious differences when applying FAME analysis, a new library (CADLIB) was successfully created using Sherlock Library Generation Software (MIDI). The amount and frequency of FAME was analyzed using library training files (n = 10 for each species), preferentially those comprising reference strains. For testing the performance of the CADLIB, clinical isolates genetically assigned to the respective species (C. albicans, n = 32; C. dubliniensis, n = 28) were chromatographically analyzed. For each isolate tested, MIS computed a similarity index (SI) indicating a hierarchy of possible strain fits. When using the newly created library CADLIB, the SIs for C. albicans andC. dubliniensis ranged from 0.11 to 0.96 and 0.53 to 0.93 (for all but one), respectively. Only three isolates of C. albicans (9.4%) were misidentified as C. dubliniensis, whereas all isolates of C. dubliniensiswere correctly identified. Resulting differentiation accuracy was 90.6% for C. albicans and 100% for C. dubliniensis. Cluster analysis and principal component analysis of the resulting FAME profiles showed two clearly distinguishable clusters matching up with two assigned species for the strains tested. Thus, the created library proved to be well suited to discriminate between these two species.

Differences among strains of Bradyrhizobium in fatty acid–methyl ester analysis

1995 ◽  
Vol 41 (11) ◽  
pp. 1038-1042 ◽  
Author(s):  
P. H. Graham ◽  
M. J. Sadowsky ◽  
S. W. Tighe ◽  
J. A. Thompson ◽  
R. A. Date ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Methyl Ester ◽  
Fatty Acid Methyl Ester ◽  
Acid Methyl Ester ◽  
Principal Component ◽  
Fatty Acid Analysis ◽  
Strain Identification ◽  
Fame Analysis ◽  
Acid Methyl

Fatty acid–methyl ester (FAME) and two-dimensional principal component analysis of 89 strains of Bradyrhizobium, most of which were from soybean, distinguished five groups of bradyrhizobia. These included one cluster containing several isolates previously designated as Bradyrhizobium elkanii, and two related clusters containing strains previously identified as belonging to Bradyrhizobium japonicum groups IA and IB. Not all of the organisms evaluated clustered with the B. japonicum and B. elkanii strains. A number of Bradyrhizobium strains isolated from soybean in Korea and northern Thailand had FAME profiles so different from the B. japonicum and B. elkanii strains as to warrant separation at the species level. A slow-growing isolate from Lupinus also had a FAME profile very different from those of the other bradyrhizobia. Results obtained in this study were generally in agreement with those obtained using other taxonomic approaches, suggesting that FAME analysis provides a relatively simple and reliable procedure for the initial characterization of Bradyrhizobium isolates.Key words: fatty acid analysis, Bradyrhizobium taxonomy, FAME analysis, strain identification.

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