Assessment of albumin binding capacity (ABiC) for characterization of albumin transport function by a new method

2001 ◽  
Vol 34 (0) ◽  
pp. 197
Author(s):  
S Klammt
Keyword(s):  
Binding Capacity ◽  
New Method ◽  
Albumin Binding ◽  
Transport Function

Assesment of Albumin Binding Capacity (ABiC) for characterization of albumin transport function by a new method

2001 ◽  
Vol 34 ◽  
pp. 197
Author(s):  
S. Klammt ◽  
B. Brinkmann ◽  
S.R. Mitzner ◽  
J. Stange ◽  
J. Loock ◽  
...  
Keyword(s):  
Binding Capacity ◽  
New Method ◽  
Albumin Binding ◽  
Transport Function

A new method for approximation of closed convex subsets of B(H)

Filomat ◽  
2017 ◽  
Vol 31 (19) ◽  
pp. 6005-6013
Author(s):  
Mahdi Iranmanesh ◽  
Fatemeh Soleimany
Keyword(s):  
Best Approximation ◽  
Numerical Range ◽  
New Method ◽  
C Algebra ◽  
Convex Subsets

In this paper we use the concept of numerical range to characterize best approximation points in closed convex subsets of B(H): Finally by using this method we give also a useful characterization of best approximation in closed convex subsets of a C*-algebra A.

On a generalization of the Rényi–Srivastava characterization of the Poisson law

2021 ◽  
Vol 58 (1) ◽  
pp. 68-82
Author(s):  
Jean-Renaud Pycke
Keyword(s):  
Life Insurance ◽  
Collective Model ◽  
New Method ◽  
Bell Polynomials ◽  
Explicit Formulas ◽  
Compound Distributions ◽  
Poisson Law ◽  
Pierre Loti

AbstractWe give a new method of proof for a result of D. Pierre-Loti-Viaud and P. Boulongne which can be seen as a generalization of a characterization of Poisson law due to Rényi and Srivastava. We also provide explicit formulas, in terms of Bell polynomials, for the moments of the compound distributions occurring in the extended collective model in non-life insurance.

scholarly journals Spectroscopic characterization of a thermodynamically stable doubly charged diatomic molecule: MgAr2+

2021 ◽  
Author(s):  
Dominik Wehrli ◽  
Matthieu Génévriez ◽  
Frédéric Merkt
Keyword(s):  
High Resolution ◽  
Diatomic Molecule ◽  
Spectroscopic Characterization ◽  
New Method ◽  
High Resolution Spectroscopy ◽  
Singly Charged ◽  
Doubly Charged

We present a new method to study doubly charged molecules relying on high-resolution spectroscopy of the singly charged parent cation, and report on the first spectroscopic characterization of a thermodynamically stable diatomic dication, MgAr2+.

scholarly journals Electrophysiological characterization of a diverse group of sugar transporters from Trichoderma reesei

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Sami Havukainen ◽  
Jonai Pujol-Giménez ◽  
Mari Valkonen ◽  
Ann Westerholm-Parvinen ◽  
Matthias A. Hediger ◽  
...  
Keyword(s):  
Trichoderma Reesei ◽  
Scientific Literature ◽  
Large Body ◽  
Comprehensive Analysis ◽  
Transport Function ◽  
Sugar Transporters ◽  
New Knowledge ◽  
Electrophysiological Characterization ◽  
Shed Light

AbstractTrichoderma reesei is an ascomycete fungus known for its capability to secrete high amounts of extracellular cellulose- and hemicellulose-degrading enzymes. These enzymes are utilized in the production of second-generation biofuels and T. reesei is a well-established host for their production. Although this species has gained considerable interest in the scientific literature, the sugar transportome of T. reesei remains poorly characterized. Better understanding of the proteins involved in the transport of different sugars could be utilized for engineering better enzyme production strains. In this study we aimed to shed light on this matter by characterizing multiple T. reesei transporters capable of transporting various types of sugars. We used phylogenetics to select transporters for expression in Xenopus laevis oocytes to screen for transport activities. Of the 18 tested transporters, 8 were found to be functional in oocytes. 10 transporters in total were investigated in oocytes and in yeast, and for 3 of them no transport function had been described in literature. This comprehensive analysis provides a large body of new knowledge about T. reesei sugar transporters, and further establishes X. laevis oocytes as a valuable tool for studying fungal sugar transporters.

scholarly journals A New Ultrasensitive Bioluminescence-Based Method for Assaying Monoacylglycerol Lipase

2021 ◽  
Vol 22 (11) ◽  
pp. 6148
Author(s):  
Matteo Miceli ◽  
Silvana Casati ◽  
Pietro Allevi ◽  
Silvia Berra ◽  
Roberta Ottria ◽  
...  
Keyword(s):  
Arachidonic Acid ◽  
Kinetic Constants ◽  
New Method ◽  
Monoacylglycerol Lipase ◽  
Enzymatic Cleavage ◽  
Highly Sensitive ◽  
Bioluminescence Assay ◽  
Identification And Characterization ◽  
In Cells

A novel bioluminescent Monoacylglycerol lipase (MAGL) substrate 6-O-arachidonoylluciferin, a D-luciferin derivative, was synthesized, physico-chemically characterized, and used as highly sensitive substrate for MAGL in an assay developed for this purpose. We present here a new method based on the enzymatic cleavage of arachidonic acid with luciferin release using human Monoacylglycerol lipase (hMAGL) followed by its reaction with a chimeric luciferase, PLG2, to produce bioluminescence. Enzymatic cleavage of the new substrate by MAGL was demonstrated, and kinetic constants Km and Vmax were determined. 6-O-arachidonoylluciferin has proved to be a highly sensitive substrate for MAGL. The bioluminescence assay (LOD 90 pM, LOQ 300 pM) is much more sensitive and should suffer fewer biological interferences in cells lysate applications than typical fluorometric methods. The assay was validated for the identification and characterization of MAGL modulators using the well-known MAGL inhibitor JZL184. The use of PLG2 displaying distinct bioluminescence color and kinetics may offer a highly desirable opportunity to extend the range of applications to cell-based assays.

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