Conversion-specific detection of DNA methylation using real-time polymerase chain reaction (ConLight-MSP) to avoid false positives

Methods ◽  
2002 ◽  
Vol 27 (2) ◽  
pp. 114-120 ◽  
Author(s):  
Keith Rand ◽  
Wenjia Qu ◽  
Thu Ho ◽  
Susan J Clark ◽  
Peter Molloy
Keyword(s):  
Dna Methylation ◽  
Polymerase Chain Reaction ◽  
Real Time ◽  
False Positives ◽  
Specific Detection ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals Development and validation of a Myxoma virus real-time polymerase chain reaction assay

2011 ◽  
Vol 24 (1) ◽  
pp. 135-137 ◽  
Author(s):  
Sarah Albini ◽  
Brigitte Sigrist ◽  
Regula Güttinger ◽  
Claude Schelling ◽  
Richard K. Hoop ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Rapid Diagnosis ◽  
Myxoma Virus ◽  
The Novel ◽  
Specific Detection ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Development And Validation ◽  
Rabbit Tissues

To aid in the rapid diagnosis of myxomatosis in rabbits, a real-time polymerase chain reaction (PCR) for the specific detection of Myxoma virus is described. Primers and probe were designed to amplify a 147-bp fragment within the Serp2 gene. The assay was able to detect 23 copies of a synthesized oligo indicating a reliable sensitivity. In addition, the real-time PCR did not detect the Rabbit fibroma virus used in myxomatosis vaccines. The novel PCR was shown to be able to detect Myxoma virus in fresh and paraffin-embedded rabbit tissues originating from myxomatosis cases from various regions in Switzerland.

scholarly journals Adenovirus of psittacine birds: investigations on isolation and development of a real-time polymerase chain reaction for specific detection

2007 ◽  
Vol 36 (6) ◽  
pp. 487-494 ◽  
Author(s):  
D. Lüschow ◽  
C. Prusas ◽  
M. Lierz ◽  
H. Gerlach ◽  
D. Soike ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Specific Detection ◽  
Chain Reaction ◽  
Polymerase Chain

Development and Comparison of Four Real-Time Polymerase Chain Reaction Systems for Specific Detection and Quantification ofZea maysL.

2004 ◽  
Vol 52 (15) ◽  
pp. 4632-4637 ◽  
Author(s):  
Marta Hernández ◽  
Marie-Noëlle Duplan ◽  
Georges Berthier ◽  
Marc Vaïtilingom ◽  
Wolfgang Hauser ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Specific Detection ◽  
Chain Reaction ◽  
Reaction Systems ◽  
Polymerase Chain ◽  
Detection And Quantification
Sign in / Sign up

Export Citation Format

Share Document