Effect of NDF concentration and physical form of fescue hay on rumen degradability, intake and rumen turn-over of cows

1991 ◽  
Vol 53 (3) ◽  
pp. 305-313 ◽  
Author(s):  
P. Susmel ◽  
M. Spanghero ◽  
B. Stefanon ◽  
C. R. Mills ◽  
C. Cargnelutti

AbstractDuring two experimental periods, eight non-lactating, rumen fistulated Simmental cows were given 2 kg/day of a cereal based concentrate and fescue hay ad libitum. The hay differed in neutral-detergent fibre concentration (LNDF: low; HNDF: high) and physical form (L: long; C: coarsely chopped). The rumen degradability of the dietary ingredients and of an extracted soya-bean meal was studied using the polyester-bag method.The estimated effective rumen degradability <DG) of dry matter (DM) was significantly higher for LNDF than for HNDF hay (0·490 v. 0·401; P < 0·01). The DM DG of the extracted soya-bean meal and the dietary concentrate were higher when incubated with LNDF than with HNDF diets (0·630 v. 0·581, P < 0·05, and 0·541 v. 0·514, respectively).Chopping the hay significantly increased the daily DM intake for HNDF diets (from 9·9 to 11·6 kg; P < 0·05), while no statistical differences were found between the other diets (10·2 and 9·6 kg, respectively for L-LNDF and C-LNDF diets). Cows given HNDF hay drank less water (42·4 v. 51·7 I/day) and total water consumption, expressed per unit of DM intake, was lower for the HNDF diets (4·1 v. 5·3 I/kg DM; P < 0·01).Dietary treatments did not affect the estimated rumen turn-over rate of hay (0·027 per h on average, k1), hay post-rumen turn-over rate (0·069 per h on average, k2) or the estimated rumen liquid turn-over rate (0·100 per h on average, kj. The calculated outflow from the rumen was lower for the HNDF diets (159·4 v. 198·7 I/day). The daily amount of estimated saliva secreted did not differ between treatments, although saliva expressed per kg DM intake was significantly lower for the HNDF diets (10·8 v. 14·8 I/kg DM; P < 0·05).The HNDF diets gave a lower post-feeding rumen pH (6·29 v. 6·58; P < 0·01), a higher concentration of n-butyrate (9·58 v. 8·32 mmol/l; P < 0·05) and a lower concentration of ammonia nitrogen (NH3-N) (80·5 v. 128·5 mg/l; P < 0·05). Of the C4 and C5 iso-acids, the rumen liquid from cows given HNDF diets had significantly lower concentrations of isobutyrate, isovalerate and n-valerate (P < 0·01). Hay physical form did not affect rumen pH, NH3-N and volatile fatty acid concentrations.

Author(s):  
M. Ellis ◽  
D.S. Parker

Synthetic lysine and fat supplements are widely used in diets for growing pigs. However, there is evidence to suggest that free lysine is more rapidly absorbed than protein bound amino acids resulting in reduced growth performance particularly under infrequent feeding regimes. In addition, the incorporation of conventional fat supplements into cereal based diets is problematic particularly where on-farm mixing of feed is carried out. One potential solution to both these problems is to use fat coated synthetic lysine sources which have recently become available and two such materials were evaluated in this study.Four dietary treatments were compared. These were a control diet (C) with soya bean meal as the protein supplement and diets in which 0.3 of the total lysine was supplied from either synthetic lysine (S) or synthetic lysine coated with a single (SC) or a double layer(DC) of hardened soya oil. All diets were barley based and were formulated to contain the same level of total lysine (0.01) and hardened soya oil.


1989 ◽  
Vol 49 (2) ◽  
pp. 249-265 ◽  
Author(s):  
N. T. Ngongoni ◽  
J. J. Robinson ◽  
R. P. Aitken ◽  
C. Fraser

ABSTRACTIn six experiments carried out on individually penned Finn Dorset ewes estimates were made of the quantities of non-ammonia nitrogen (NAN) reaching the abomasum and truly digested in the small intestine. For experiments 1 and 2 which each involved 33 ewes given a complete diet of milled hay and concentrates supplemented with varying amounts of a good-quality fish meal during late pregnancy, the overall regression estimate for the daily amount of truly digested NAN required for zero N retention (ewe plus conceptus) was 438 mg/kg M0·75 (441 and 434 for experiments 1 and 2 respectively). The coefficients for the efficiency of utilization of increments of truly digested NAN and maternal tissue N for net N accretion in the conceptus were 0·48 (s.e. 0·039) and 0·84 (s.e. 0·127) respectively.In experiment 3, 24 lactating ewes had their diet supplemented with either soya-bean meal or fish meal. There was no effect of protein source on the amounts of NAN reaching the abomasum in ewes fitted with an abomasal cannula or on milk yield and these observations confirmed the unusually high degradability (measured by the polyester bag technique) of the fish-meal protein in the rumen. For experiments 4, 5 and 6 a total of 36 ewes were used to test the efficiency with which the NAN truly digested in the small intestine was used for the synthesis of milk protein. In the absence of data on the possible contribution of body tissue N to milk N the coefficient for the apparent efficiency of utilization of truly digested NAN when the ewes were given a basal diet containing approximately 10 g crude protein per MJ metabolizable energy was 0·63. For those ewes receiving the basal diet supplemented with either soya-bean meal, fish meal or blood meal the coefficients for the efficiency of utilization for the production of milk N of the increments in truly digested NAN supplied by the three protein sources were 0·61, 0·54 and 0·29 respectively. It is suggested that the low coefficient for blood meal may be due to its low content of methionine.


2008 ◽  
Vol 51 (1) ◽  
pp. 57-65 ◽  
Author(s):  
Manuel García-Ulloa Gomez ◽  
Luis Alberto López-Aceves ◽  
Jesús Trinidad Ponce-Palafox ◽  
Hervey Rodríguez-González ◽  
José Luis Arredondo-Figueroa

Growth parameters (standard length, weight, specific growth rate and daily weight gain) of prawn Macrobrachium tenellum juveniles fed 40% crude protein isoproteic diets substituting fish meal with soya bean meal at various levels (20, 40, 60, 80 and 100%) were evaluated for 45 days under laboratory conditions. Experimental diets were compared with a 100% fish meal based diet. Total survival was recorded for all the treatments at the end of the experiment. There were no significant differences (p > 0.05) for all the growth parameters among the dietary treatments. The initial mean weight (0.36 ± 0.10 g) increased almost three times (1.00 ± 0.13 g) after 45 days. The final specific growth fluctuated from 1.82% BW/d for the 60% soya bean meal inclusion diet, to 2.62% for the 100% fish meal diet. The mean final survival was 91.66%. Growth performance of M. tenellum juveniles was not affected by the dietary soya bean meal levels tested.


1990 ◽  
Vol 51 (2) ◽  
pp. 235-244 ◽  
Author(s):  
R. F. Cody ◽  
J. J. Murphy ◽  
D. J. Morgan

ABSTRACTFour concentrate supplements differing in crude protein (CP) and undegradable protein (UDP) content were offered to 16 lactating Friesian cows together with grass silage (dry matter (DM) 196 g/kg, pH 4·38, CP 160 g/kg DM, in vitro DM digestibility 0·68)ad libitumin a Latin-square trial with 3-week periods. The supplement treatments were: (1) barley 122 g CP per kg DM, degradability (dg) 0·77; (2) barley/soya-bean meal 210 g CP per kg DM, dg 0·69; (3) barley/soya-bean meal/fish meal 190 g CP per kg DM, dg 0·61; (4) barley/soya-bean meal/fish meal 219 g CP per kg DM, dg 0·59. Supplements were given at 8 kg/day. Total daily intakes of silage (kg DM), CP and UDP (g) on treatments 1 to 4 were 7·77, 2087, 375; 8·35, 2804, 655; 8·29, 2676, 717; 8·70, 2917, 826, respectively. Milk yield (kg/day) and yields of fat, protein and lactose (g/day) on the four treatments were 21·3, 791, 617, 984; 23·0, 816, 688, 1055; 23·0, 818, 696, 1050; 23·6, 813, 735, 1071 for treatments 1 to 4 respectively. Yield and concentration of protein and lactose were significantly lower on treatment 1 than on the other treatments, while the of blood metabolites indicated treatment effects on blood glucose, non-esterified fatty acids, plasma protein and urea. Digestibility of organic matter and non-ammonia nitrogen (NAN) flow to the abomasum (g/day), measured in sheep given a fixed silage/supplement ratio at maintenance, were 0·81 and 18·4, 0·81 and 20·8, 0·82 and 21·4, 0·82 and 22·4 for treatments 1 to 4 respectively. The NAN flow was significantly greater on treatment 4 than on treatment 1.


1989 ◽  
Vol 61 (1) ◽  
pp. 113-121 ◽  
Author(s):  
J. A. Rooke ◽  
D. G. Armstrong

1. In a 4x4 Latin square design experiment, four cattle were given grass silage in two meals per d to satisfy maintenance energy requirements. In addition, sucrose (170 g/kg silage dry matter (DM)) was infused intraruminally at a constant rate with no nitrogen supplementation; with the infusion intraruminally of either casein (23 g/kg silage DM) or urea (8 g/kg silage DM); or with soya-bean meal (64 g/kg silage DM) fed in two equal portions.2. Samples of duodenal digesta representative of a 24 h period were obtained using chromium-EDTA and ytterbium acetate for flow estimation and35S as a marker of microbial N entering the small intestine. Samples of rumen fluid were also taken for estimation of rumen pH and concentrations of ammonia-N and volatile fatty acids. Estimates of apparent organic matter (OM) and N digestibility and of the rates of silage DM and N disappearance from porous synthetic-fibre bags incubated in the rumen were also made.3. The N supplements had no significant effects on rumen pH, concentrations of volatile fatty acids, their molar proportions or the disappearance of DM or N from porous synthetic-fibre bags. N supplementation increased rumen ammonia-N concentrations (urea,P< 0.05; casein, soya-bean meal, not significant).4. N supplementation had no significant effects on the digestion of OM, acid-detergent fibre or soluble carbohydrate.5. Infusion of casein increased the quantities of total non-ammonia-N (not significant) and microbial N (P< 0.05) entering the small intestine daily and the efficiency of rumen microbial N synthesis (not significant). Giving soya-bean meal twice daily resulted in marginal increases in the quantities of non-ammonia-N and microbial N entering the small intestine, while infusing urea intraruminally had no effect.


1992 ◽  
Vol 54 (3) ◽  
pp. 333-339 ◽  
Author(s):  
R. W. J. Steen

AbstractTwo experiments were carried out to evaluate extracted soya-bean meal, fish meal and maize gluten feed as protein supplements for calves offered grass silage ad libitum. The five treatments used in experiment 1 consisted of 1·4 kg per head daily of concentrate dry matter (DM) consisting of (g/kg) 50 molasses and 25 minerals and vitamins plus (1) 925 barley (B), (2) 700 B and 225 extracted soya-bean meal (SBM), (3) 785 B and 140 fish meal (FM), (4) 925 maize gluten feed (MGF) and (5) 2·25 kg DM per head daily of concentrate (1). Those used in experiment 2 consisted of 1·4 kg per head daily of concentrate DM consisting of (g/kg) (1) 1000 B, (2) 800 B and 200 SBM, (3) 860 B and 140 FM, (4) 1000 MGF and (5) 2·25 kg DM per head daily of B. In each experiment the diets were offered to 70 calves which were initially 131 and 130 kg live weight, for periods of 14 and 13 weeks in experiments 1 and 2 respectively. The silages contained 155 and 154 g crude protein (CP) per kg DM; 45 and 77 g ammonia-nitrogen per kg total nitrogen and 698 and 726 g digestible organic matter per kg DM in experiments 1 and 2. Concentrates 1 and 5 contained 104 to 110 g CP per kg DM and concentrates 2, 3 and 4 contained 195 to 204 g CP per kg DM in both experiments. For diets 1 to 5 respectively silage DM intakes were 2·31,2·50,2·53,2·59 and 1 -61 (s.e. 0·097) kg/day; live-weight gains were 0·84,0·98,1 -01, 0·88 and 0·95 (s.e. 0·032) kg/day in experiment 1 and 2·38, 2·53, 2·54, 2·82 and 1·77 (s.e. 0·045) kg/day and 0·90,1·04,1·03,1·05 and 0·99 (s.e. 0·038) kg/day for experiment 2. It is concluded that the use of soya-bean meal rather than fish meal did not affect silage intake or animal performance. Replacing mixtures of barley and soya-bean meal or barley and fish meal with an equal quantity of isonitrogenous maize gluten feed did not affect animal performance in one experiment but significantly reduced it in the other experiment.


1986 ◽  
Vol 43 (2) ◽  
pp. 201-209 ◽  
Author(s):  
J. P. Alawa ◽  
G. Fishwick ◽  
J. J. Parkins ◽  
R. G. Hemingway ◽  
T. C. Aitchison

ABSTRACTThree experiments, designed as three simultaneous 4 × 4 Latin squares (21-day feeding periods), were conducted using pregnant beef cows. Diets were based on barley straw given ad libitum together with 1·7 kg dry matter (DM) of either molassed sugar-beet pulp (MSBP) (experiment 1), unmolassed sugarbeet pulp (USBP) (experiment 2), or rolled barley (BARLEY) (experiment 3). In the respective experiments, MSBP, USBP and BARLEY were given either alone, to provide about 200 g crude protein (CP) per day (CONTROL) or were supplemented with urea (U), untreated soya-bean meal (SBM) or formaldehyde-treated soya-bean meal (FT-SBM) to provide a further 200 g CP per day. The contrasting dietary treatments in the three experiments provided intakes of rumen-degradable protein (RDP) ranging from 16 to 388 g/day.All protein supplements improved overall straw DM intakes, the improvement being significant for U only (P < 0·01). A linear relationship between the daily intake of RDP (g) and straw DM (kg) was obtained. The regression equation was:DM intake = 5·03 + 0·0035 × RDP (R2 = 0·703; residual s.d. 0·561; d.f. 35; P < 0·001).All protein supplements also improved the apparent digestibility of straw organic matter (OM) (P < 0·05) and the metabolizable energy (ME) obtained from straw (P < 0·01). Blood urea was increased due to supplementation with urea (P < 0·001), SBM (P < 0·01) and FT-SBM (P < 0·05). Supplementation with BARLEY appeared to improve straw DM intake over MSBP and USBP but this was not reflected in improved ME intake from straw.


1968 ◽  
Vol 10 (2) ◽  
pp. 193-199 ◽  
Author(s):  
H. Swan ◽  
G. E. Lamming

Thirty-two yearling Friesian steers were used in a 2 × 2 factorial experiment. The treatment variables were source of supplementary nitrogen and physical form of the diet. The diets contained 30% ground barley straw, ground maize, a nitrogen supplement, and minerals and vitamins. Urea was compared with soya bean meal as the nitrogen supplement and both diets were given as cubes or meal. There was no significant difference between treatments in daily live-weight gain, feed intake or commercial carcass acceptability.Digestibility trials with wethers showed the apparent digestibility of nitrogen and dry matter of the diet containing urea to be significantly lower than that of the diet containing soya bean meal (nitrogen 72·8 ν. 76·3, P < 0·001; dry matter 70·2 ν. 75·3, P < 0·01). The results are discussed in relation to the digestibility of the diets used, the source of supplementary nitrogen and the physical form of the diet.


1983 ◽  
Vol 49 (1) ◽  
pp. 119-127 ◽  
Author(s):  
A. B. Mcallan ◽  
R. H. Smith

1. Steers fitted with simple rumen, abomasal and sometimes duodenal cannulas were given isonitrogenous and isoenergetic diets consisting of pellets containing equal amounts of rolled barley and chopped straw together with tapioca alone (B), or with some tapioca replaced by coarse soya-bean meal (M) or finely ground soya-bean flour (F). Diet B had an estimated rumen degradable nitrogen (RDN): metabolizable energy (ME) value of approximately 0·8 g/MJ. The corresponding value for each of the supplemented diets was 1·3–1·4. Diets were given at two levels of intake designed respectively to provide ME intakes to support weight gains of 0·5 kg/d (L) and 1·0 kg/d (H). Chromic oxide and polyethylene glycol were given as indigestible markers. Values were calculated for flows (g/24 h) at the abomasum of organic matter (OM), non-ammonia-N (NAN) and microbial (bacterial)-N (MN). Estimates of MN were based on RNA and α-ε;-diaminopimelic acid (DAP) measurements. The steers had low rumen protozoal counts throughout the whole experiment with fewer than 8000 ciliate protozoa/ml of rumen fluid.2. Proportions of OM apparently digested between the mouth and abomasum were significantly lower for H than for L intakes but showed no other significant differences. Mean estimated efficiency of microbial protein synthesis was approximately 17 g N/kg OM apparently digested in the rumen for the LB diet, but was significantly greater at approximately 26 g N/kg OM apparently digested for the HB diet. The protein-supplemented diets gave values of 31–32 which were significantly greater than the value for the LB but not the HB diet.3. Amounts of feed nitrogenous constituents surviving between the mouth and the abomasum were estimated from NAN minus MN corrected for endogenous N. Values for the proportions of feed N degraded, derived from these estimates, were 0·71, 0·66, 0·72, 0·69, 0·67 and 0·70 for diets LB, HB, LM, HM, LF and HF respectively. There were no significant differences due to level of feeding, supplementary protein or the physical form of that protein.4. Estimates of MN flows at the abomasum based on RNA determinations in fresh samples did not differ significantly from estimates based on DAP determinations. However, storage of aqueous suspensions of mixed rumen bacteria by deep-freezing or freeze-drying resulted in significant losses of RNA. No such losses were observed for abomasal digesta. This meant that if samples were stored by these means before analysis, the use of RNA as a microbial marker was vitiated.5. In some experiments digesta samples were taken from the duodenum as well as the abomasum. Estimates of total N flows did not differ significantly between these sites.


2003 ◽  
Vol 77 (1) ◽  
pp. 73-78 ◽  
Author(s):  
J. L. Hartke ◽  
G. A. Apgar ◽  
K. E. Griswold ◽  
B. N. Jacobson ◽  
T. L. Rosenthal ◽  
...  

AbstractA total of 276 crossbred pigs were used in three trials to determine if feeding spray-dried animal plasma (SDAP) in simple phase 1 piglet diets with differing soya-bean meal (SBM) levels can influence performance or immunoglobulin G (IgG) levels specific to the antigenic soya proteins, glycinin and β-conglycinin. Pigs were blocked according to initial body weight and equalized across treatments according to gender and ancestry. Blocks of pigs were then randomly assigned to one of three treatments in a completely randomized block design. The dietary treatments were as follows: (1) 10% +: 100 g/kg SBM with 75 g/kg SDAP; (2) 20% +: 200 g/kg SBM with 75 g/ kg SDAP; (3) 20%-: 200 g/kg SBM without SDAP. Pigs were given treatment diets for 14 days, followed by a common diet for the duration of the trials (35 days). Average daily gain (ADG) and gain to food (G: F) ratios were not affected by dietary treatments. Average daily food intake (ADFI), however, was greater for pigs given 10% + as compared with 20% + and 20%- (P < 0.05) during days 0 to 14 post weaning. Addition of SDAP increased ADFI (P < 0.01) and tended to increase ADFI when added to diets containing 200 g/kg of soya-bean meal (P = 0.06). No differences were detected in soya-specific IgG levels during any collection period. These data suggest that SDAP addition to simple phase 1 piglet diets increased ADFI during the first 2 weeks, but did not alter ADG or food efficiency. There was no indication that SDAP addition altered IgG titres against the soya-bean proteins, glycinin and β-conglycinin.


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