Some observations on the distribution and origin of nitrogen in sheep faeces

1969 ◽  
Vol 73 (1) ◽  
pp. 99-111 ◽  
Author(s):  
V. C. Mason

SUMMARY1. Three methods, based on treatment with neutral detergent or acid detergent, or involving ultrasonic disintegration, are described and compared for the direct estimation of undigested dietary nitrogen in individual samples of sheep faeces. Estimates of the true digestibility of the nitrogen in several sheep diets derived from analyses performed with these methods agreed well with each other, and were in accord with published estimates, derived by extrapolation techniques. Two other methods, based on treatment with phenol–acetic acid–water, and lysozyme–trypsin, respectively, were found to be unsuitable for such estimates.2. The quantitative distribution of nitrogen between undigested dietary residues, bacterial residues, endogenous debris residues and the water soluble fraction was determined chemically. It was concluded that 57–81% of the non-dietary faecal nitrogen was associated with bacterial material.3. Indirect evidence suggested that most of the bacterial nitrogen in faeces originated in the rumen.

Holzforschung ◽  
2002 ◽  
Vol 56 (4) ◽  
pp. 341-347 ◽  
Author(s):  
Y. Uraki ◽  
M. Morito ◽  
T. Kishimoto ◽  
Y. Sano

Summary The water-soluble fraction (WS) from waste liquor of atmospheric acetic acid pulping was analyzed in order to explore its potential utilization as a carbon source for bacterial cellulose (BC) production. The unpurified WS that contained colored compounds had appreciable antibacterial activity and was therefore not used as a carbon source for BC production with the bacteria Acetobacter xylinum ATCC 10245 and 53582. However, the bacteria produced BC from purified WS of both fir and birch after activated carbon treatment. The yields of BC were much lower compared to those using glucose as a typical carbon source in the Hestrin-Schramm (HS) medium. In order to improve the cellulose production, monosaccharides derived from hemicellulose sugars in WS were modified. The WS from softwood was reduced by catalytic reduction, i.e. mannose, the major component of softwood monosaccharides derived from hemicelluloses, was converted to mannitol. In the case of hardwood WS, xylose was isomerized with glucose isomerase to xylulose. The yield of BC was considerably enhanced by both modifications of the WS. The yield of BC using the modified WS from the pulping of fir was superior to that using glucose as carbon source.


2014 ◽  
Vol 91 (5) ◽  
pp. 473-481 ◽  
Author(s):  
Guiai Jiao ◽  
Xiangjin Wei ◽  
Gaoneng Shao ◽  
Lihong Xie ◽  
Zhonghua Sheng ◽  
...  

1957 ◽  
Vol 35 (4) ◽  
pp. 241-250 ◽  
Author(s):  
W. G. Martin ◽  
J. E. Vandegaer ◽  
W. H. Cook

Livetin, the major water-soluble protein of hen egg yolk, was found to contain three major components having mobilities of −6.3, −3.8, and −2.1 cm.2 sec.−1 volt−1 at pH 8, µ 0.1, and these have been designated α-, β-, and γ-livetin respectively. The α- and β-livetins were separated and purified electrophoretically after removal of γ-livetin by precipitation from 37% saturated ammonium sulphate or 20% isopropanol. The α-, β-, and mixed livetins resembled pseudoglobulins in solubility but γ-livetin was unstable and this loss of solubility has, so far, prevented its characterization. Molecular weights determined by light scattering, osmotic pressure, and Archibald sedimentation procedure yielded respectively: 8.7, 7.8, and 6.7 × 104 for α-livetin, and 4.8, 5.0, and4.5 × 104 for β-livetin. Under suitable conditions of sedimentation and electrophoresis, egg yolk has been shown to contain three components having the same behavior as the three livetins of the water-soluble fraction.


Chemosphere ◽  
2010 ◽  
Vol 78 (11) ◽  
pp. 1301-1312 ◽  
Author(s):  
S.M. Rodrigues ◽  
B. Henriques ◽  
J. Coimbra ◽  
E. Ferreira da Silva ◽  
M.E. Pereira ◽  
...  

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