Attempts at in vitro fertilization and culture of in vitro matured oocytes in sei (Balaenoptera borealis) and Bryde's (B. edeni) whales

Zygote ◽  
2009 ◽  
Vol 17 (1) ◽  
pp. 19-28 ◽  
Author(s):  
M. M. U. Bhuiyan ◽  
Y. Suzuki ◽  
H. Watanabe ◽  
K. Matsuoka ◽  
Y. Fujise ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Bovine Serum Albumin ◽  
Embryo Culture ◽  
Culture Medium ◽  
Cumulus Cells ◽  
Protein Supplementation ◽  
Vitro Fertilization ◽  
Embryo Culture Medium ◽  
Morula Stage

SummaryThe cumulus–oocyte–complexes (COCs) recovery rates with respect to reproductive status per sei (Balaenoptera borealis) and Bryde's (B. edeni) whales were determined in Experiment 1. The number of COCs recovered ranged from 16.0 to 30.6 and from 6.7 to 26.8 per sei and Bryde's whales, respectively. The effects of COCs grades and protein supplementation in embryo culture medium on development of in vitro fertilized (IVF) embryos were evaluated in sei and Bryde's whales in Experiment 2. The COCs were classified into either Grade A (COCs with five or more layers of compact cumulus cells) or Grade B (COCs with less than five layers of compact or expanded cumulus cells) before being cultured for IVM. The cleavage (12.0 to 19.5%), 4-cell (8.0 to 12.0%) and 8-cell (4.0 to 8.0%) formation rates in sei whales did not vary significantly between embryos derived from either grade A or B oocytes and between embryos cultured in either fetal whale serum (FWS)- or bovine serum albumin (BSA)-supplemented medium. The cleavage (4.0 to 14.8%), 4-cell (0.0 to 7.5%) and 8-cell (0.0 to 2.6%) formation rates in Bryde's whales did not vary significantly between embryos derived from either grade A or B oocytes and between embryos cultured in either FWS- or BSA-supplemented medium. The grade B oocytes cultured in FWS-supplemented medium developed to morula stage (1.1%) in sei whales. In conclusion, the present study indicates that IVF in sei whales is possible to achieve cleaved embryos developing to morula stage. This is the first in vitro embryo production attempt in sei and Bryde's whales.

Isolation of a histamine releasing factor from human embryo culture medium after in-vitro fertilization

1987 ◽  
Vol 2 (4) ◽  
pp. 341-344 ◽  
Author(s):  
Roberta Cocchiara ◽  
Giovana Di Trapani ◽  
Antonina Azzolina ◽  
Giuseppe Albeggiani ◽  
Rosanna Ciriminna ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Embryo Culture ◽  
Human Embryo ◽  
Culture Medium ◽  
Vitro Fertilization ◽  
Embryo Culture Medium ◽  
Human Embryo Culture

scholarly journals Does Embryo Culture Medium Influence the Health and Development of Children Born after In Vitro Fertilization?

PLoS ONE ◽  
2016 ◽  
Vol 11 (3) ◽  
pp. e0150857 ◽  
Author(s):  
Céline Bouillon ◽  
Roger Léandri ◽  
Laurent Desch ◽  
Alexandra Ernst ◽  
Céline Bruno ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Embryo Culture ◽  
Culture Medium ◽  
Vitro Fertilization ◽  
Embryo Culture Medium ◽  
Health And Development ◽  
Medium Influence

scholarly journals Influence of embryo culture medium on incidence of ectopic pregnancy in in vitro fertilization

2015 ◽  
Vol 104 (6) ◽  
pp. 1442-1445 ◽  
Author(s):  
Shengli Lin ◽  
Rong Li ◽  
Xiaoying Zheng ◽  
Hongbin Chi ◽  
Xiulian Ren ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Ectopic Pregnancy ◽  
Embryo Culture ◽  
Culture Medium ◽  
Vitro Fertilization ◽  
Embryo Culture Medium

scholarly journals LIF level is a predictive marker for clinical pregnancy following IVF-ET of patients with fallopian tube obstruction

2020 ◽  
Author(s):  
Zewu Li ◽  
Ruimei Li ◽  
Huiying Dai ◽  
Xiaoyun Li ◽  
Xiao Han ◽  
...  
Keyword(s):  
Pregnancy Outcome ◽  
Embryo Culture ◽  
Culture Medium ◽  
Predictive Marker ◽  
Clinical Pregnancy ◽  
Vitro Fertilization ◽  
Embryo Culture Medium ◽  
Infertile Patients ◽  
Ivf Et

Abstract Background: Leukemia inhibitory factor (LIF) has played a vital role in a series of reproductive events, including follicle growth, embryo growth and differentiation. However, it is unclear whether the level of LIF in embryo culture medium can be used as a marker for clinical pregnancy. In this study, we aimed to investigate whether LIF level in embryo culture medium can act as a predictive marker for pregnancy outcome of in vitro fertilization-embryo transfer (IVF-ET) in infertile patients due to tubal problems.Methods: A total of 104 infertile patients due to tubal problems underwent IVF-ET treatment. The patients were divided into two groups according to whether they were clinically pregnant. The level of LIF in the embryo culture medium was measured, and the correlation between LIF level and embryo quality and clinical pregnancy outcome was analyzed. The embryo culture medium was collected on the day of blastocyst transplantation.Results: Compared to non-pregnant group, LIF level in the embryo culture medium on the day of blastocyst transplantation was significantly higher in the pregnant group.Conclusions: LIF level in the embryo culture medium may be used as a non-invasive auxiliary biomarker for predictive clinical pregnancy in infertile patients with tubal problems that using single blastocyst transfer method.

scholarly journals PS48 can replace bovine serum albumin in pig embryo culture medium, and improve in vitro embryo development by phosphorylating AKT

2015 ◽  
Vol 82 (4) ◽  
pp. 315-320 ◽  
Author(s):  
Lee D. Spate ◽  
Alana Brown ◽  
Bethany K. Redel ◽  
Kristin M. Whitworth ◽  
Randall S. Prather
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Embryo Development ◽  
Embryo Culture ◽  
Bovine Serum ◽  
Culture Medium ◽  
Embryo Culture Medium

scholarly journals LIF level is a predictive marker for clinical pregnancy following IVF-ET of patients with fallopian tube obstruction

2020 ◽  
Author(s):  
Zewu Li ◽  
Ruimei Li ◽  
Huiying Dai ◽  
Xiaoyun Li ◽  
Xiao Han ◽  
...  
Keyword(s):  
Pregnancy Outcome ◽  
Embryo Culture ◽  
Culture Medium ◽  
Predictive Marker ◽  
Clinical Pregnancy ◽  
Vitro Fertilization ◽  
Embryo Culture Medium ◽  
Infertile Patients ◽  
Ivf Et

Abstract Background: Leukemia inhibitory factor (LIF) has played a vital role in a series of reproductive events, including follicle growth, embryo growth and differentiation. However, it is unclear whether the level of LIF in embryo culture medium can be used as a marker for clinical pregnancy. In this study, we aimed to investigate whether LIF level in embryo culture medium can act as a predictive marker for pregnancy outcome of in vitro fertilization-embryo transfer (IVF-ET) in infertile patients due to tubal problems.Methods: A total of 104 infertile patients due to tubal problems underwent IVF-ET treatment. The patients were divided into two groups according to whether they were clinically pregnant. The level of LIF in the embryo culture medium was measured, and the correlation between LIF level and embryo quality and clinical pregnancy outcome was analyzed. The embryo culture medium was collected on the day of blastocyst transplantation.Results: Compared to non-pregnant group, LIF level in the embryo culture medium on the day of blastocyst transplantation was significantly higher in the pregnant group.Conclusions: LIF level in the embryo culture medium may be used as a non-invasive auxiliary biomarker for predictive clinical pregnancy in infertile patients with tubal problems that using single blastocyst transfer method.

scholarly journals The effects of supplemented sericin on in vitro maturation and preimplantation development of mouse embryos: An experimental study

2021 ◽  
pp. 921-928
Author(s):  
Omid Banafshi ◽  
Sherko Nasseri ◽  
Leila Farhadi ◽  
Masoud Alasvand ◽  
Mohammad Bagher Khadem-Erfan ◽  
...  
Keyword(s):  
Embryo Culture ◽  
In Vitro Maturation ◽  
Culture Medium ◽  
Control Group ◽  
Vitro Fertilization ◽  
In Vitro Development ◽  
Embryo Culture Medium ◽  
Vitro Development

Background: Mouse embryo culture condition is an essential part of transgenic, reproductive and developmental biology laboratories. Mouse embryonic culture media may have a high risk of serum contamination with pathogens.  Objective: To investigate the effect of sericin as an embryo culture medium supplement on in vitro maturation (IVM), in vitro fertilization (IVF), and development of the preimplantation embryo in mice. Materials and Methods: The effects of sericin at three concentrations (subgroups) of 0.1%, 0.5%, and 1% as a medium supplement on IVM, IVF, and in vitro development of mouse embryos were separately investigated and compared with a sericin-free (control) group. The cumulative effect of the three concentrations was evaluated for IVM + in vitro development and IVF + in vitro development as follow-up groups. Results: In the IVM group, compared to the control group, the number of oocysts reaching the MII stage was significantly higher when 1% sericin was used (161/208 = 77.4%). No significant results were observed in the IVF and in vitro development groups with different concentrations of sericin compared to the control group. Among the follow-up groups, in the IVM + in vitro development group, the number of oocytes was higher after passing the IVM and IVF and reaching the blastocysts stage when 1% sericin was used, compared with other sericin subgroups. A significant difference was also noted when compared with the control group (p = 0.048). The IVF + in vitro development study group, on the other hand, did not show any significant relationship. Conclusion: It can be concluded that 1% sericin can be used as a supplement in mouse embryo cultures to improve the IVM rate. Also, based on the findings, sericin appears to be an effective supplement which can have a positive effect on the development of embryos derived from IVM. Key words: Sericin, In vitro maturation, In vitro fertilization, Preimplantation embryo, Culture medium, Mice.

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