Effect of adding different levels of probiotic on in vitro gas production

2009 ◽  
Vol 2009 ◽  
pp. 187-187
Author(s):  
M Besharati ◽  
A Taghizadeh ◽  
A Ansari
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Nutritive Value ◽  
Cellulose Degradation ◽  
Gas Production ◽  
Nutrient Digestibility ◽  
Production Performance ◽  
Attractive Alternative ◽  
Kinetics Of ◽  
Different Levels

Probiotics present an attractive alternative to the use of chemical and hormonal promoters in the livestock growth production industry. Preparations that contain micro-organisms have been safely used for many years and are generally accepted by both the farmer and the final consumer. Saccharomyces cerevisiae supplementation in ruminant diets can increase DMI, production performance, cellulose degradation, and nutrient digestibility (Callaway and Martin, 1997). The gas measuring technique has been widely used for the evaluation of nutritive value of feeds. Gas measurement provides useful data on digestion kinetics of both soluble and insoluble fractions of feedstuffs (Getachew et al., 1998). In the gas method, kinetics of fermentation can be studied on a single sample and therefore a relatively small amount of sample is required or a larger number of samples can be evaluated at time. The purpose of this study was to study effect of adding different levels of Saccharomyces cerevisiae on in vitro gas production from a dried grape by-product.

Effects of Saccharomyces cerevisiae on the nutritive value of lucerne hay assessed with the in vitro gas production technique

2009 ◽  
Vol 2009 ◽  
pp. 186-186
Author(s):  
A Ansari ◽  
A Taghizadeh ◽  
H Janmohamadi ◽  
G Zarini
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Nutritive Value ◽  
Gas Production ◽  
Nutrient Digestibility ◽  
Feed Additives ◽  
Production Performance ◽  
Yeast Culture ◽  
Fast Method ◽  
Production Technique

Yeast culture (Saccharomyces cerevisiae) has been widely used as a dietary supplement in ruminants. Saccharomyces cerevisiae supplementation in ruminant diets can increase dry matter intake, production performance, cellulose degradation, and nutrient digestibility (Callaway and Martin, 1997). In vitro techniques are routinely used to evaluate the nutritional quality of feeds because of their convenience, adaptability and efficiency. The in vitro gas production technique is an accurate and fast method to evaluate the nutritive value of feeds and it can be a good alternative method instead of in vivo trials to evaluate commercial feed additives. The objective of this study was to evaluate the effects of different levels of inclusion of Saccharomyces cerevisiae on the nutritive value of lucerne hay using the in vitro gas production technique.

scholarly journals Chemical-bromatological composition and in vitro ruminal kinetics of sugar cane silage with maniçoba

2018 ◽  
Vol 40 (1) ◽  
pp. 42569
Author(s):  
Francisco Allan Leandro de Carvalho ◽  
Percivaldo Xavier Resende ◽  
Clístenes Amorim Benicio ◽  
Jackson De Oliveira Siqueira ◽  
Daniel Ribeiro Menezes ◽  
...  
Keyword(s):  
Sugar Cane ◽  
Nutritive Value ◽  
Degradation Kinetics ◽  
Gas Production ◽  
Metabolizable Energy ◽  
Randomized Design ◽  
In Vitro Gas Production ◽  
Inclusion Level ◽  
Kinetics Of

The objective this study was to evaluate the effect of maniçoba supplementation in sugar cane silage with respect to chemical-bromatological composition and the in vitro degradation kinetics of the silage. This experiment was conducted in a completely randomized design with four treatments (maniçoba levels: 0, 20, 30, and 40%) and six repetitions. Silage samples were analyzed for their chemical-bromatological composition, digestible energy, metabolizable energy, total digestible nutrients, in vitro gas production and degradability parameters. The silage with higher inclusion level had better bromatological composition (p < 0.05) than the silage without maniçoba for CP, NDF, ADF and MM (6.49, 56.64, 38.66 and 4.52% versus 2.21, 70.96, 49.95 and 2.78%). Higher ME content (2.35 MJ kg-1 MS versus 1.85 MJ kg-1 MS), DE (2.87 Mcal kg-1 MS versus 2.25 Mcal kg-1 MS) and TDN (65.16% versus 51.11%), respectively. The highest values for gas production were also observed in silage with added maniçoba due to higher NFC content (34.87%). With an increase in the proportion of maniçoba, there was an increase in the soluble a fraction, b fraction, and thus a higher effective degradability of dry matter (46.56%). The addition of maniçoba improves the nutritive value of sugarcane silage.  

Nutritive evaluation of heat-treated cottonseed with in vitro gas production

2003 ◽  
Vol 2003 ◽  
pp. 175-175
Author(s):  
A. R. Foroughi ◽  
A. A. Naserian ◽  
R. Valizadeh ◽  
M. Danesh Mesgaran ◽  
A. Mirhady
Keyword(s):  
Nutritive Value ◽  
Gas Production ◽  
Value Evaluation ◽  
In Vitro Gas Production ◽  
Heat Treated ◽  
Gas Measurements ◽  
Biological Methods ◽  
Digestion Kinetics ◽  
Kinetics Of

Biological methods are more meaningful since microorganisms and enzymes are more sensitive to factors influencing the rate and extent of digestion than are chemical methods (Getachew and et al,1997).Gas measurements provides a useful data on digestion kinetics of both soluble and insoluble fractions of feedstuffs. The objective of this experiment was to determine nutritive value evaluation of heat-treated cottonseed with in vitro gas production.

In vitro gas production of pomegranate peel treated with urea, with and without PVP

2005 ◽  
Vol 2005 ◽  
pp. 223-223
Author(s):  
R. Feizi ◽  
A. Ghodratnama ◽  
M. Zahedifar ◽  
M. Danesh Mesgaran ◽  
M. Raisianzadeh
Keyword(s):  
Nutritive Value ◽  
Bitter Taste ◽  
Gas Production ◽  
Fruit Peel ◽  
Pomegranate Peel ◽  
In Vitro Gas Production ◽  
By Products ◽  
Different Levels

Pomegranate by-products (peel and seed) contain about 40-45 percent of the fruit’s weight. The rind of the fruit (peel),when dried, is brown outside, yellow inside, hard, dry, brittle, in irregular fragments, inodorous, and with a very astringent, somewhat bitter taste. Analysis of pomegranate peel (PP) is shown that it contains 18.8 percent of tannin, 17.1 of mucilage, 10.8 of extractive matter, 30 of lignin, a trace of resin, and 29.9 of moisture. However, little information is available on PP nutritive value for ruminants. It is poor in protein and rich in tannins. Tannins components of the peel prevents its optimal use. The objective of this experiment was to evaluate the effect of different levels of urea (U) on in vitro gas production with and without added polyvinylpolypyrrolidone (PVP) to ensiled pomegranate peel (EPP).

In vitro rumen gas production kinetics, hydrocyanic acid concentration and fermentation characteristics of fresh cassava root and feed block sulfur concentration

2020 ◽  
Vol 60 (5) ◽  
pp. 659 ◽  
Author(s):  
Gamonmas Dagaew ◽  
Anusorn Cherdthong ◽  
Metha Wanapat ◽  
Pin Chanjula
Keyword(s):  
Nitrogen Concentration ◽  
Gas Production ◽  
Nutrient Digestibility ◽  
Hydrocyanic Acid ◽  
In Vitro Digestibility ◽  
High Concentration ◽  
Cassava Root ◽  
Fermentation Liquor ◽  
Kinetics Of

Context Feeding ruminants with fresh cassava roots (FCR) is limited because they have a high concentration of hydrocyanic acid (HCN). Thus, it was hypothesised that receiving a feed block containing high sulfur (FBS) would reduce hydrocyanic acid (HCN) in FCR and improve rumen fermentation and nutrient digestibility in animals. Aims The goal of the present work was to study the influence of the ratio of FCR to rice straw (RS) together with FBS on kinetics of gas production, HCN concentration, fermentation characteristics and nutrient digestibility, using in vitro technique. Methods The experimental design was a 4 × 2 factorial arrangement in a completely randomised design, with three replications per treatment. Factor A was the FCR to RS ratio, which was 100:0, 60:40, 40:60 or 0:100. Factor B was sulfur, which was provided as two concentrations in FBS (2% and 4% DM). Gas production was recorded after incubation, at 0, 0.5, 1, 2, 4, 6, 8, 12, 18, 24, 48, 72 and 96 h. Fermentation liquor was collected and determined for kinetics of gas production, HCN concentration, fermentation characteristics and nutrient digestibility. Key results Cassava root to RS ratio influenced the cumulative gas production after 96 h. Inclusion of sulfur in the FBS at 4% increased the cumulative gas production, when compared with inclusion at 2%. The gas production from degradable fraction (b) and the rate of gas production (c) were significantly different among the treatments with different FCR:RS ratios, whereas there was no difference between using 2% and 4% sulfur in the FBS. The HCN concentration in fermentation liquor increased with an increasing proportion of FCR. Furthermore, inclusion of sulfur in the feed block at 4% reduced HCN concentration by 42.8%, when compared with inclusion at 2% (P &lt; 0.05). Ammonia-nitrogen concentration was significantly different among the FCR:RS-ratio treatments and was reduced when the proportion of FCR was decreased (P &lt; 0.05). In vitro digestibility was significantly increased with an increasing proportion of FCR. Increasing the proportion of FCR with 4% of sulfur in the FBS significantly increased in vitro DM digestibility, compared with 2% sulfur. Increasing the FCR:RS ratio with 4% of sulfur in the FBS increased the proportion of propionic acid (P &lt; 0.05). Conclusions Using a high FCR:RS ratio (100:0 or 60:40) with 4% sulfur in the FBS enhanced kinetics of gas production, propionic molar proportion, nutrient digestibility, and HCN detoxification by rumen microbes in an in vitro trial. Implications An in vivo study should be encouraged to verify the results and obtain more data.

Relationship between in vitro gas production and near infrared reflectance spectra of gliricidia provenances

1996 ◽  
Vol 1996 ◽  
pp. 226-226
Author(s):  
S.J. Lister ◽  
M.S. Dhanoa ◽  
J.L. Stewart ◽  
M. Gill
Keyword(s):  
Nutritive Value ◽  
Near Infrared ◽  
Gas Production ◽  
Infrared Reflectance ◽  
Near Infrared Reflectance ◽  
In Vitro Gas Production ◽  
The Relationship ◽  
Kinetics Of

The chemical composition of tropical forages is generally more complex than that of temperate forages, yet the need for simple methods to predict nutritive value is greater. Near Infrared Reflectance (NIR) spectra could provide a possible solution. NIR has been used to predict both in vitro and in vivo digestibility (Murray, 1993). Direct calibration with in vivo parameters is ideal, but factors such as different types of forages, animals and management conditions have to be taken into consideration. The gas production technique, which may be considered as an in vitro counterpart of the dacron bag technique, provides information relating to the fermentable fractions and kinetics of a forage and these parameters can be related to the NIR spectra. The aim of this study was to investigate the relationship between NIR spectra and cumulative gas production and derived parameters in tree leaves.

scholarly journals The effect of a candidate feed additive derived from the essential oils of Pinus merkusii (jungh. & de vriese) and Melaleuca leucadendra (l.) on the kinetics of gas production and methane emitted during in-vitro ruminal fermentation

2021 ◽  
Vol 33 ◽  
pp. 04009
Author(s):  
Asih Kurniawati ◽  
Muhlisin Muhsin Al Anas
Keyword(s):  
Essential Oils ◽  
Methane Production ◽  
Gas Production ◽  
Nutrient Digestibility ◽  
Feed Additives ◽  
Feed Additive ◽  
Ruminal Fermentation ◽  
Total Potential ◽  
Kinetics Of

The study was designed to determine the effect of a candidate natural feed additive on the kinetics of gas production as a representation of feed degradability and methane produced during rumen fermentation. Three blends of essential oil (BEO) as candidates for feed additives were formulated using pine and eucalyptus essential oils in the following ratios: 75:25, 50:50, and 25:75 for BEO1, BEO2, and BEO3, respectively. Every BEO was added to the batch fermentation system at dosages of 0, 100, and 200 l/l in the medium. Furthermore, an in vitro gas production technique was used to simulate rumen feed fermentation. According to the gas production kinetics, all BEO additives did not affect the total potential gas produced, as well as the potential gas produced from the soluble and insoluble substrate. The rates of gas production were similar among treatments. Furthermore, the addition of BEO did not affect the total volume of gas produced during fermentation. Meanwhile, BEO1 at 200 l/l dose and BEO 3 at 100 l/l dose significantly reduced methane production (P0.05). In conclusion, the BEO1 and BEO 3 at dosages of 200 and 100 l/l, respectively, had the potential as a feed additive to reduce methane production without a negative effect on nutrient digestibility.

scholarly journals Relationship between In vivo, In vitro Parameters and Chemical Composition to Predict the Nutritive Value of Some Legume Forages

2021 ◽  
Author(s):  
A. Aïssa ◽  
C. Ben Mustapha ◽  
M.R. Alvir ◽  
M. Hadj Ayed ◽  
I.E.A. Znaïdi ◽  
...  
Keyword(s):  
Crude Protein ◽  
Nutritive Value ◽  
Chemical Constituents ◽  
Protein Digestibility ◽  
Insoluble Fraction ◽  
Gas Production ◽  
Metabolizable Energy ◽  
Kinetics Of

Background: Many in vivo and laboratory methods have been used to evaluate ruminant feeds. The objective of this experiment was to determine feed intake, digestibility, kinetics of gas production, in vitro organic matter digestibility (OMDv) and metabolizable energy (ME) of Trifolium alexandrinum (T. alexandrinum) and Medicago sativa (M. sativa) at different maturity stages through in vivo and in vitro methods to elaborate predictive equations from chemical constituents. Methods: In vivo assay was carried out with two groups of five rams male kipped in metabolism cage. Samples of forage, refusal and feces were collected and processed for chemical analysis. In vitro gaz production technique was performed on forage samples.Result: The bud and early bloom stage of M. sativa recorded the highest digestibility values of the chemical component (P less than 0.05). The OMDv of M. sativa was 60.65 and 68.26% (P less than 0.01) for early bloom stage and bud stage, respectively. Crude protein digestibility (CPD) was positively correlated with crude protein rate (R2=0.83, P less than 0.05). The gas production from the insoluble fraction “b” fraction showed a positive correlation with acid detergent fiber (ADF) (R2=0.999, P less than 0.001). However, cumulative gas production at 24h and 48 h of incubation were negatively correlated (P less than 0.05) with the ADF rate (R2= -0.98 and -0.97, respectively). 

Relationship between in vitro gas production and near infrared reflectance spectra of gliricidia provenances

1996 ◽  
Vol 1996 ◽  
pp. 226-226
Author(s):  
S.J. Lister ◽  
M.S. Dhanoa ◽  
J.L. Stewart ◽  
M. Gill
Keyword(s):  
Nutritive Value ◽  
Near Infrared ◽  
Gas Production ◽  
Infrared Reflectance ◽  
Near Infrared Reflectance ◽  
In Vitro Gas Production ◽  
The Relationship ◽  
Kinetics Of

The chemical composition of tropical forages is generally more complex than that of temperate forages, yet the need for simple methods to predict nutritive value is greater. Near Infrared Reflectance (NIR) spectra could provide a possible solution. NIR has been used to predict both in vitro and in vivo digestibility (Murray, 1993). Direct calibration with in vivo parameters is ideal, but factors such as different types of forages, animals and management conditions have to be taken into consideration. The gas production technique, which may be considered as an in vitro counterpart of the dacron bag technique, provides information relating to the fermentable fractions and kinetics of a forage and these parameters can be related to the NIR spectra. The aim of this study was to investigate the relationship between NIR spectra and cumulative gas production and derived parameters in tree leaves.

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