Gradient elution in reversed-phase HPLC-separation of macromolecules

1983 ◽  
Vol 55 (14) ◽  
pp. 1412A-1430A ◽  
Author(s):  
L. R. Snyder ◽  
M. A. Stadalius ◽  
M. A. Quarry
Keyword(s):  
Gradient Elution ◽  
Reversed Phase ◽  
Reversed Phase Hplc ◽  
Hplc Separation

Reversed-Phase HPLC Separation of Human Serum Employing a Novel Saw-Tooth Gradient:  Toward Multidimensional Proteome Analysis

2004 ◽  
Vol 3 (6) ◽  
pp. 1149-1154 ◽  
Author(s):  
Daniel L. Morris, ◽  
Jennifer N. Sutton ◽  
Robert G. Harper ◽  
Aaron T. Timperman
Keyword(s):  
Human Serum ◽  
Proteome Analysis ◽  
Reversed Phase ◽  
Reversed Phase Hplc ◽  
Hplc Separation

scholarly journals Quantification of glycosaminoglycans by reversed-phase HPLC separation of fluorescent isoindole derivatives

Glycobiology ◽  
2005 ◽  
Vol 16 (1) ◽  
pp. 65-72 ◽  
Author(s):  
Daniel R. Studelska ◽  
Kari Giljum ◽  
Lynda M. McDowell ◽  
Lijuan Zhang
Keyword(s):  
Reversed Phase ◽  
Reversed Phase Hplc ◽  
Hplc Separation

Allantoin as a marker of oxidative stress in human erythrocytes

2008 ◽  
Vol 46 (9) ◽  
Author(s):  
Roman Kand'ár ◽  
Pavla Žáková
Keyword(s):  
Oxidative Stress ◽  
Renal Failure ◽  
Uric Acid ◽  
Chronic Renal Failure ◽  
Gradient Elution ◽  
Reversed Phase ◽  
Control Group ◽  
Uric Acid Concentration ◽  
Reversed Phase Hplc

Abstract: Uric acid is the final product of purine metabolism in humans. It was determined that this compound has important antioxidative properties and it may be oxidized to allantoin by various reactive oxygen species. Therefore, the measurement of allantoin may be useful for the determination of oxidative stress in humans.: We measured allantoin and uric acid in human plasma and erythrocytes obtained from patients with chronic renal failure before hemodialysis (n=30) and blood donors (n=30). We used a method based on selective isolation of allantoin from deproteinized plasma and erythrocyte lysate samples on AG 1-X8 resin and its derivatization to glyoxylate-2, 4-dinitrophenylhydrazone. Separation of glyoxylate-2, 4-dinitrophenylhydrazone from interfering substances was achieved on reversed phase HPLC with gradient elution and UV/VIS detection at 360 nm. Uric acid was determined by reversed phase HPLC with UV/VIS detection at 292 nm.: We found significant differences in allantoin and uric acid concentration between the patients with chronic renal failure and the control group both in plasma (20.5±6.5 μmol/L and 323.9±62.9 μmol/L vs. 2.1±1.1 μmol/L and 270.1±62.3 μmol/L, p<0.05) and erythrocytes [82.8±39.1 nmol/g hemoglobin (Hb) and 110.7±28.8 nmol/g Hb vs. 20.1±6.1 nmol/g Hb and 82.1±23.7 nmol/g Hb, p<0.05].: Significant higher levels of allantoin in both plasma and erythrocytes of patients with chronic renal failure indicate that allantoin may be used as a good marker of oxidative stress.Clin Chem Lab Med 2008;46:1270–4.

Reversed phase HPLC separation of fossil chlorins

1983 ◽  
Vol 6 (10) ◽  
pp. 573-574 ◽  
Author(s):  
G. Liebezeit ◽  
J. Bartel
Keyword(s):  
Reversed Phase ◽  
Reversed Phase Hplc ◽  
Hplc Separation
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