Four-Channel Photothermal Plate Reader for High-Throughput Nanoparticle-Amplified Immunoassay

2020 ◽  
Vol 92 (24) ◽  
pp. 15705-15710
Author(s):  
Wenwen Liu ◽  
Xiaoying Wang ◽  
Chuanqi Tai ◽  
Wenyuan Yan ◽  
Ruichuang Yu ◽  
...  
Keyword(s):  
High Throughput ◽  
Plate Reader

scholarly journals High-Throughput Chemometric Quality Assessment of Extra Virgin Olive Oils Using a Microtiter Plate Reader

Sensors ◽  
2019 ◽  
Vol 19 (19) ◽  
pp. 4169
Author(s):  
Huihui He ◽  
Weiying Lu
Keyword(s):  
Quality Assessment ◽  
High Throughput ◽  
Latent Variables ◽  
Scatter Correction ◽  
Principal Component ◽  
Microtiter Plate ◽  
Food Ingredients ◽  
Virgin Olive Oils ◽  
Olive Oils ◽  
Plate Reader

A commercially available microtiter plate reader was applied as a high-throughput counterpart of ultraviolet-visible (UV–Vis) spectrophotometer to identify the producing location of extra virgin olive oils (EVOOs). Multiplicative scatter correction and the first derivative was used to denoise the UV–Vis spectra and eliminate the effects of background drift. The spectra were analyzed using chemometrics methods including the principal component analysis (PCA) and the partial least squares-discriminant analysis (PLS-DA). The PLS-DA model on full spectra using 5 latent variables showed a classification accuracy of 97.92% by cross-validation. The overall results demonstrated that the use of a UV–Vis spectrophotometer based on the microtiter plate reader combined with chemometrics can be applied to the quality assessment of EVOOs. It is demonstrated that the microtiter plate reader can be a high-throughput tool in the quality assessment of food ingredients.

A High-Throughput Screening Campaign for Detection of Ca2+–Activated K+ Channel Activators and Inhibitors Using a Fluorometric Imaging Plate Reader–Based Tl+-Influx Assay

2013 ◽  
Vol 11 (3) ◽  
pp. 163-172 ◽  
Author(s):  
Susanne Jørgensen ◽  
Tino Dyhring ◽  
David T. Brown ◽  
Dorte Strøbæk ◽  
Palle Christophersen ◽  
...  
Keyword(s):  
High Throughput ◽  
High Throughput Screening ◽  
K Channel ◽  
Imaging Plate ◽  
Plate Reader

scholarly journals An Effective Tool for Homogeneous Assays Using a Simple Robot Arm with Stackers—TRISTAN

2002 ◽  
Vol 7 (1) ◽  
pp. 89-94
Author(s):  
Kazuyoshi Yajima ◽  
Takanori Ohgaru ◽  
Yoshifumi Hashimoto ◽  
Naoki Suto ◽  
Toru Okuda
Keyword(s):  
Cost Effectiveness ◽  
High Throughput ◽  
High Throughput Screening ◽  
Integrated System ◽  
Robotic Systems ◽  
Robot Arm ◽  
Parameter Setting ◽  
Plate Reader ◽  
Homogeneous Assay ◽  
Homogeneous Assays

The Twister &! RapidPlate Integrated System by TANabe, or TRISTAN, consists of a 96-channel dispenser (Rapid-Plate 96), a plate reader (V-MAX), and a simple robot arm (Twister). We developed TRISTAN for effectively conducting a homogeneous assay. Although this system accommodates fewer than 20 microplates, it has several advantages over conventional robotic systems for high-throughput screening in the following aspects: parameter setting, running time, hardware errors, manpower, and cost-effectiveness. The system proved to be effective and efficient for homogeneous assays.

A Thallium Transport FLIPR-Based Assay for the Identification of KCC2-Positive Modulators

2010 ◽  
Vol 15 (2) ◽  
pp. 177-184 ◽  
Author(s):  
Di Zhang ◽  
Sujatha M. Gopalakrishnan ◽  
Gail Freiberg ◽  
Carol S. Surowy
Keyword(s):  
High Throughput ◽  
High Throughput Screening ◽  
Screening Method ◽  
Inhibitory Neurons ◽  
Imaging Plate ◽  
Neuronal Excitation ◽  
Inhibitory Mechanisms ◽  
Pharmacological Studies ◽  
Plate Reader ◽  
Transport Assay

KCC2, potassium chloride cotransporter 2, is expressed exclusively in the CNS (on inhibitory neurons) and plays a major role in maintaining appropriately low intracellular chloride levels that ensure inhibitory actions of GABAA and glycine receptors. As such, it plays a pivotal role in inhibitory mechanisms that control neuronal excitation in the CNS. KCC2 downregulation has been implicated in various excitatory disorders, such as epilepsy and neuropathic pain. Positive modulators of KCC2 expression or activity may thus provide effective therapy for these disorders. However, the identification of such agents is hindered by the lack of a high-throughput screening method. Here the authors report the development of a fluorescence-based thallium (Tl+) transport assay using a Fluorometric Imaging Plate Reader (FLIPR), in which KCC2 activity is assessed by measuring the initial rate of KCC2-mediated Tl+ transport/influx. The authors demonstrate Tl+/Cl− cotransport by KCC2, which exhibits a high apparent affinity for Tl+ and dependency on the presence of the Cl− ion. Pharmacological studies revealed anticipated effects and potencies of known KCC-positive (NEM, staurosporine) and KCC-negative (DIOA, furosemide) modulators. The authors demonstrate that the assay is robust and reproducible and can be employed in high-throughput screening for positive modulators of KCC2 as potential therapeutic agents.

An optimized high-throughput fluorescence plate reader-based RSV neutralization assay

2018 ◽  
Vol 260 ◽  
pp. 34-40 ◽  
Author(s):  
Yong-Peng Sun ◽  
Wei Zhang ◽  
Qin-Jian Zhao ◽  
Jian-Li Cao ◽  
Lu-Jing Zhang ◽  
...  
Keyword(s):  
High Throughput ◽  
Neutralization Assay ◽  
Plate Reader ◽  
Fluorescence Plate Reader

scholarly journals New Device for High-Throughput Viability Screening of Flow Biofilms

2010 ◽  
Vol 76 (13) ◽  
pp. 4136-4142 ◽  
Author(s):  
Michael R. Benoit ◽  
Carolyn G. Conant ◽  
Cristian Ionescu-Zanetti ◽  
Michael Schwartz ◽  
A. Matin
Keyword(s):  
Fluid Flow ◽  
High Throughput ◽  
Fluorescent Protein ◽  
Accurate Determination ◽  
Microtiter Plate ◽  
Rapid Screening ◽  
Microfluidic Channels ◽  
Screening Methods ◽  
New Device ◽  
Plate Reader

ABSTRACT Control of biofilms requires rapid methods to identify compounds effective against them and to isolate resistance-compromised mutants for identifying genes involved in enhanced biofilm resistance. While rapid screening methods for microtiter plate well (“static”) biofilms are available, there are no methods for such screening of continuous flow biofilms (“flow biofilms”). Since the latter biofilms more closely approximate natural biofilms, development of a high-throughput (HTP) method for screening them is desirable. We describe here a new method using a device comprised of microfluidic channels and a distributed pneumatic pump (BioFlux) that provides fluid flow to 96 individual biofilms. This device allows fine control of continuous or intermittent fluid flow over a broad range of flow rates, and the use of a standard well plate format provides compatibility with plate readers. We show that use of green fluorescent protein (GFP)-expressing bacteria, staining with propidium iodide, and measurement of fluorescence with a plate reader permit rapid and accurate determination of biofilm viability. The biofilm viability measured with the plate reader agreed with that determined using plate counts, as well as with the results of fluorescence microscope image analysis. Using BioFlux and the plate reader, we were able to rapidly screen the effects of several antimicrobials on the viability of Pseudomonas aeruginosa PAO1 flow biofilms.

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