scholarly journals Interaction between Presenilin 1 and Ubiquilin 1 as Detected by Fluorescence Lifetime Imaging Microscopy and a High-throughput Fluorescent Plate Reader

2006 ◽  
Vol 281 (36) ◽  
pp. 26400-26407 ◽  
Author(s):  
Anne V. Thomas ◽  
Lauren Herl ◽  
Robert Spoelgen ◽  
Mikko Hiltunen ◽  
Phill B. Jones ◽  
...  
Keyword(s):  
High Throughput ◽  
Fluorescence Lifetime ◽  
Presenilin 1 ◽  
Fluorescence Lifetime Imaging Microscopy ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging ◽  
Plate Reader

scholarly journals A Review of New High-Throughput Methods Designed for Fluorescence Lifetime Sensing From Cells and Tissues

2021 ◽  
Vol 9 ◽  
Author(s):  
Aric Bitton ◽  
Jesus Sambrano ◽  
Samantha Valentino ◽  
Jessica P. Houston
Keyword(s):  
Flow Cytometry ◽  
High Throughput ◽  
Fluorescence Lifetime ◽  
Fluorescence Lifetime Imaging Microscopy ◽  
Fluorescence Lifetime Imaging ◽  
Intrinsic Properties ◽  
Time Resolved ◽  
Lifetime Imaging ◽  
The Past ◽  
Biological Studies

Though much of the interest in fluorescence in the past has been on measuring spectral qualities such as wavelength and intensity, there are two other highly useful intrinsic properties of fluorescence: lifetime (or decay) and anisotropy (or polarization). Each has its own set of unique advantages, limitations, and challenges in detection when it comes to use in biological studies. This review will focus on the property of fluorescence lifetime, providing a brief background on instrumentation and theory, and examine the recent advancements and applications of measuring lifetime in the fields of high-throughput fluorescence lifetime imaging microscopy (HT-FLIM) and time-resolved flow cytometry (TRFC). In addition, the crossover of these two methods and their outlooks will be discussed.

scholarly journals Direct organelle thermometry with fluorescence lifetime imaging microscopy in single myotubes

2016 ◽  
Vol 52 (24) ◽  
pp. 4458-4461 ◽  
Author(s):  
Hideki Itoh ◽  
Satoshi Arai ◽  
Thankiah Sudhaharan ◽  
Sung-Chan Lee ◽  
Young-Tae Chang ◽  
...  
Keyword(s):  
Fluorescence Lifetime ◽  
Heat Production ◽  
Fluorescence Lifetime Imaging Microscopy ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging ◽  
C2c12 Myotube

FLIM of ER thermo yellow and non-targeted mCherry reveals the Ca2+-dependent heat production localized to SR in C2C12 myotube.

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