Development of Fluorescent Ganglioside GD3 and GQ1b Analogs for Elucidation of Raft-Associated Interactions

2020 ◽  
Vol 85 (24) ◽  
pp. 15998-16013
Author(s):  
Miku Konishi ◽  
Naoko Komura ◽  
Yuya Hirose ◽  
Yuki Suganuma ◽  
Hide-Nori Tanaka ◽  
...  
Keyword(s):  
Ganglioside Gd3

scholarly journals The Potential Role of Human NME1 in Neuronal Differentiation of Porcine Mesenchymal Stem Cells: Application of NB-hNME1 as a Human NME1 Suppressor

2021 ◽  
Vol 22 (22) ◽  
pp. 12194
Author(s):  
Jin Hyoung Cho ◽  
Won Seok Ju ◽  
Sang Young Seo ◽  
Bo Hyun Kim ◽  
Ji-Su Kim ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Neuronal Differentiation ◽  
Nucleoside Diphosphate Kinase ◽  
Inhibitory Effect ◽  
Human Macrophage ◽  
Potential Candidate ◽  
Xenograft Rejection ◽  
Ganglioside Gd3

This study aimed to investigate the effects of the human macrophage (MP) secretome in cellular xenograft rejection. The role of human nucleoside diphosphate kinase A (hNME1), from the secretome of MPs involved in the neuronal differentiation of miniature pig adipose tissue-derived mesenchymal stem cells (mp AD-MSCs), was evaluated by proteomic analysis. Herein, we first demonstrate that hNME1 strongly binds to porcine ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 1 (pST8SIA1), which is a ganglioside GD3 synthase. When hNME1 binds with pST8SIA1, it induces degradation of pST8SIA1 in mp AD-MSCs, thereby inhibiting the expression of ganglioside GD3 followed by decreased neuronal differentiation of mp AD-MSCs. Therefore, we produced nanobodies (NBs) named NB-hNME1 that bind to hNME1 specifically, and the inhibitory effect of NB-hNME1 was evaluated for blocking the binding between hNME1 and pST8SIA1. Consequently, NB-hNME1 effectively blocked the binding of hNME1 to pST8SIA1, thereby recovering the expression of ganglioside GD3 and neuronal differentiation of mp AD-MSCs. Our findings suggest that mp AD-MSCs could be a potential candidate for use as an additive, such as an immunosuppressant, in stem cell transplantation.

Ganglioside GD3 shedding by human gliomas

1991 ◽  
Vol 109 (1-2) ◽  
pp. 34-36 ◽  
Author(s):  
O. Nakamura ◽  
M. Iwamori ◽  
M. Matsutani ◽  
K. Takakura
Keyword(s):  
Human Gliomas ◽  
Ganglioside Gd3

Simple Construction of Neu5Ac(α2-8)Neu5Ac and Total Synthesis of Ganglioside GD3

1996 ◽  
Vol 15 (7) ◽  
pp. 857-878 ◽  
Author(s):  
Tadao Kondo ◽  
Toshiyuki Tomoo ◽  
Hiroyuki Abe ◽  
Minoru Isobe ◽  
Toshio Goto
Keyword(s):  
Total Synthesis ◽  
Simple Construction ◽  
Ganglioside Gd3

Development of macroglial cells in rat cerebellum. II. An in situ immunohistochemical study of oligodendroglial lineage from precursor to mature myelinating cell

Development ◽  
1988 ◽  
Vol 102 (2) ◽  
pp. 409-425 ◽  
Author(s):  
R. Reynolds ◽  
G.P. Wilkin
Keyword(s):  
White Matter ◽  
Basic Protein ◽  
Molecular Layer ◽  
Final Destination ◽  
Rat Cerebellum ◽  
Cell Layers ◽  
Cryostat Sections ◽  
Ganglioside Gd3 ◽  
4Th Ventricle

Using immunofluorescence with a panel of antibodies that recognize antigens expressed by oligodendroglia, the myelin-producing cells of the CNS, at different stages of differentiation from precursor to mature cell, we have investigated the development of cells of this lineage in cryostat sections of rat cerebellum. Our results are consistent with the view that glial precursors, identified by their expression of the ganglioside GD3, arise in the subependymal layers of the 4th ventricle and migrate to their final position in the cerebellum via the superior medullary velum, and to some extent the peduncles. As the cells reach their final destination they make the transition to recognizable galactocerebroside (GC)-expressing oligodendroglia, via a GD3+/GC+ intermediate. The myelin-associated protein 2′,3′-cyclic nucleotide 3′-phosphohydrolase (CNP) appears at the same time as GC, whereas myelin basic protein (MBP) is expressed 2–3 days after GC and CNP, immediately prior to myelin formation. A very clear progression of oligodendroglial differentiation was observed from the SMV into the base of the cerebellum, up into the white matter (WM) tracts of the folia, and then away from this central white matter into the granule cell and Purkinje cell layers, and finally the molecular layer. The time delay between the expression of GC, CNP and MBP was the same for oligodendroglia in all of these layers, suggesting the presence of an intrinsic clock controlling the initial expression of these myelin components. The early appearance of CNP in oligodendroglia suggests a role for this protein in the early stages of myelinogenesis.

Ganglioside GD3 shedding by human malignant melanoma cells

1989 ◽  
Vol 44 (1) ◽  
pp. 155-160 ◽  
Author(s):  
Helga Bernhard ◽  
Karl-Hermann Meyer Zum Büschenfelde ◽  
Wolfgang G. Dippold
Keyword(s):  
Malignant Melanoma ◽  
Melanoma Cells ◽  
Human Malignant Melanoma ◽  
Ganglioside Gd3

scholarly journals Ganglioside GD3 synthase (GD3S), a novel cancer drug target

2018 ◽  
Vol 8 (5) ◽  
pp. 713-720 ◽  
Author(s):  
Jinyi Liu ◽  
Xiangjin Zheng ◽  
Xiaocong Pang ◽  
Li Li ◽  
Jinhua Wang ◽  
...  
Keyword(s):  
Drug Target ◽  
Cancer Drug ◽  
Gd3 Synthase ◽  
Ganglioside Gd3

The Ganglioside GD3 as the Greek Goddess Hecate: Several Faces Turned Towards as Many Directions

IUBMB Life ◽  
2005 ◽  
Vol 57 (7) ◽  
pp. 477-482 ◽  
Author(s):  
Florence Malisan ◽  
Roberto Testi
Keyword(s):  
Ganglioside Gd3
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