Melibiose permease and .alpha.-galactosidase of Escherichia coli: identification by selective labeling using a T7 RNA polymerase/promoter expression system

Biochemistry ◽  
1990 ◽  
Vol 29 (3) ◽  
pp. 690-696 ◽  
Author(s):  
Thierry Pourcher ◽  
Martine Bassilana ◽  
Hemanta K. Sarkar ◽  
H. Ronald Kaback ◽  
Gerard Leblanc
Keyword(s):  
Escherichia Coli ◽  
Rna Polymerase ◽  
Expression System ◽  
T7 Rna Polymerase ◽  
Selective Labeling ◽  
Alpha Galactosidase ◽  
Rna Polymerase Promoter

A new T7 RNA polymerase-driven expression system induced via thermoamplification of a recombinant plasmid carrying a T7 promoter-Escherichia coli lac operator

Gene ◽  
1994 ◽  
Vol 142 (1) ◽  
pp. 61-66 ◽  
Author(s):  
Marina I. Lebedeva ◽  
Ekaterina V. Rogozhkina ◽  
Nikolay A. Tsyba ◽  
Sergey V. Mashko
Keyword(s):  
Escherichia Coli ◽  
Rna Polymerase ◽  
Recombinant Plasmid ◽  
Expression System ◽  
T7 Rna Polymerase ◽  
T7 Promoter ◽  
Lac Operator

scholarly journals T7Max transcription system

2021 ◽  
Author(s):  
Christopher Deich ◽  
Brock Cash ◽  
Wakana Sato ◽  
Judee Sharon ◽  
Lauren Aufdembrink ◽  
...  
Keyword(s):  
Protein Expression ◽  
Rna Polymerase ◽  
Expression System ◽  
T7 Rna Polymerase ◽  
Dna Templates ◽  
Transcription System ◽  
In Vitro Systems ◽  
In Vitro Protein Expression ◽  
Rna Polymerase Promoter

Efficient cell-free protein expression from linear DNA templates has remained a challenge primarily due to template degradation. Here we present a modified T7 RNA polymerase promoter that acts to significantly increase the yields of both transcription and translation within in vitro systems. The modified promoter, termed T7Max, recruits standard T7 RNA polymerase, so no protein engineering is needed to take advantage of this method. This technique could be used with any T7 RNA polymerase- based in vitro protein expression system. Unlike other methods of limiting linear template degradation, the T7Max promoter increases transcript concentration in a T7 transcription reaction, providing more mRNA for translation.

scholarly journals Overproduction of human α-2b interferon in a soluble form in Escherichia coli cells using the bacteriophage T7 RNA polymerase-base expression system

2003 ◽  
Vol 19 (4) ◽  
pp. 367-373 ◽  
Author(s):  
I. Yu. Slavchenko ◽  
E. V. Boreyko ◽  
N. V. Vorobey ◽  
S. I. Chernykh ◽  
V. A. Kordyum
Keyword(s):  
Escherichia Coli ◽  
Rna Polymerase ◽  
Expression System ◽  
T7 Rna Polymerase ◽  
Soluble Form ◽  
Bacteriophage T7 ◽  
Escherichia Coli Cells

Novel expression system for Corynebacterium acetoacidophilum and Escherichia coli based on the T7 RNA polymerase-dependent promoter

2013 ◽  
Vol 97 (17) ◽  
pp. 7755-7766 ◽  
Author(s):  
Md. Javed Equbal ◽  
Preeti Srivastava ◽  
Gopal Prasad Agarwal ◽  
Jahar Kanti Deb
Keyword(s):  
Escherichia Coli ◽  
Rna Polymerase ◽  
Expression System ◽  
T7 Rna Polymerase

scholarly journals Stringent control in Escherichia coli applies also to transcription by T7 RNA polymerase.

1987 ◽  
Vol 262 (9) ◽  
pp. 3940-3943
Author(s):  
M. Yamagishi ◽  
J.R. Cole ◽  
M. Nomura ◽  
F.W. Studier ◽  
J.J. Dunn
Keyword(s):  
Escherichia Coli ◽  
Rna Polymerase ◽  
T7 Rna Polymerase ◽  
Stringent Control

Tests of a model of specific contacts in T7 RNA polymerase-promoter interactions

Biochemistry ◽  
1995 ◽  
Vol 34 (2) ◽  
pp. 666-672 ◽  
Author(s):  
Charlie Schick ◽  
Craig T. Martin
Keyword(s):  
Rna Polymerase ◽  
T7 Rna Polymerase ◽  
Rna Polymerase Promoter

Continuous protein synthesis system with Escherichia coli S30 extract containing endogenous T7 RNA polymerase

1993 ◽  
Vol 15 (8) ◽  
Author(s):  
Norihiro Nishimura ◽  
Yoshihisa Kitaoka ◽  
Akio Mimura ◽  
Yoshimasa Takahara
Keyword(s):  
Escherichia Coli ◽  
Protein Synthesis ◽  
Rna Polymerase ◽  
T7 Rna Polymerase ◽  
Synthesis System ◽  
S30 Extract ◽  
Protein Synthesis System

High-level transcription of large gene regions: a novel T7 RNA-polymerase-based system for expression of functional hydrogenases in the phototrophic bacterium Rhodobacter capsulatus

2005 ◽  
Vol 33 (1) ◽  
pp. 56-58 ◽  
Author(s):  
T. Drepper ◽  
S. Arvani ◽  
F. Rosenau ◽  
S. Wilhelm ◽  
K.-E. Jaeger
Keyword(s):  
Rna Polymerase ◽  
Rhodobacter Capsulatus ◽  
Expression System ◽  
Active Form ◽  
T7 Rna Polymerase ◽  
High Level Synthesis ◽  
Phototrophic Bacterium ◽  
Large Gene ◽  
High Level ◽  
Complex Enzymes

High-level synthesis of complex enzymes like bacterial [NiFe] hydrogenases, in general, requires an expression system that allows concerted expression of a large number of genes. So far, it has not been possible to overproduce a hydrogenase in a stable and active form by using a customary expression system. Therefore we started to establish a new, T7-based expression system in the phototrophic bacterium Rhodobacter capsulatus. The beneficial properties of this bacterial host in combination with the unique capacity of T7 RNA polymerase to synthesize long transcripts will allow the high-level synthesis and assembly of active hydrogenase as well as other complex enzymes in the near future.

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