scholarly journals Surface-Modified P(HEMA-co-MAA) Nanogel Carriers for Oral Vaccine Delivery: Design, Characterization, and In Vitro Targeting Evaluation

2014 ◽  
Vol 15 (7) ◽  
pp. 2725-2734 ◽  
Author(s):  
Matilde Durán-Lobato ◽  
Brenda Carrillo-Conde ◽  
Yasmine Khairandish ◽  
Nicholas A. Peppas
Keyword(s):  
Oral Vaccine ◽  
Vaccine Delivery ◽  
Surface Modified

scholarly journals Comparison and process optimization of PLGA, chitosan and silica nanoparticles for potential oral vaccine delivery

2019 ◽  
Vol 10 (8) ◽  
pp. 493-514 ◽  
Author(s):  
Muhammad K Amin ◽  
Joshua S Boateng
Keyword(s):  
Silica Nanoparticles ◽  
Process Parameters ◽  
Functional Performance ◽  
Oral Vaccine ◽  
Polymer Concentration ◽  
Vaccine Delivery ◽  
Inorganic Materials ◽  
Protein Encapsulation ◽  
Scanning Transmission

Aim: The study compared performance of nanoparticles prepared from synthetic organic, natural organic and inorganic materials as vaccine delivery platforms. Materials & methods: Various formulation (concentration, polymer/silica:surfactant ratio, solvent) and process parameters (homogenization speed and time, ultrasonication) affecting functional performance characteristics of poly(lactic- co-glycolic acid) (PLGA), chitosan and silica-based nanoparticles containing bovine serum albumin were investigated. Nanoparticles were characterized using dynamic light scattering, x-ray diffraction, scanning/transmission electron microscopy, Fourier transform infrared spectroscopy and in vitro protein release. Results: Critical formulation parameters were surfactant concentration (PLGA, silica) and polymer concentration (chitosan). Optimized nanoparticles were spherical in shape with narrow size distribution and size ranges of 100–300 nm (blank) and 150–400 nm (protein loaded). Protein encapsulation efficiency was 26–75% and released within 48 h in a sustained manner. Conclusion: Critical formulation and process parameters affected size of PLGA, chitosan and silica nanoparticles and protein encapsulation, while silica produced the smallest and most stable nanoparticles.

In Vitro Mineralization and Cell Adhesion on Surface Modified Poly (2 - hydroxy ethyl methacrylate-co-methyl methacrylate

2006 ◽  
Vol 309-311 ◽  
pp. 493-496 ◽  
Author(s):  
G.S. Sailaja ◽  
T.V. Kumari ◽  
Yoshiyuki Yokogawa ◽  
H.K. Varma
Keyword(s):  
Cell Adhesion ◽  
Calcium Phosphate ◽  
Methyl Methacrylate ◽  
Radical Copolymerization ◽  
Sem Analysis ◽  
Free Radical Copolymerization ◽  
In Vitro Mineralization ◽  
Atr Spectroscopy ◽  
Surface Modified

Poly(2-hydroxyethyl methacrylate-co- methyl methacrylate) HM, was synthesized by free radical copolymerization, cross-linked with ethylene glycol dimethacrylate and phosphorylated. The phosphate coupling was ensured by ATR spectroscopy. The in vitro mineralization ability of the phosphorylated HM (designated as PHM) was investigated by studying the nucleation and growth of calcium phosphate on its surface by immersing in simulated body fluid (SBF) solution. The coating morphology was studied by SEM and the Ca/P ratio of the coating by EDX analysis. The cell adhesion behaviour of PHM was studied by seeding Human osteosarcoma (HOS) cells for one week followed by SEM analysis along with HM as control. It was observed that HOS cells exhibited biomineralization of calcium phosphate on the surface of HM as well as on PHM with a significantly higher amount on the surface of PHM as observed by von kossa staining method. The results show that PHM is capable of in vitro mineralization under simulated physiological condition, promotes cell adhesion by providing an excellent cell friendly surface and it exhibits biomineralization of calcium phosphate in presence of HOS cells.

Bilosomes: the answer to oral vaccine delivery?

2001 ◽  
Vol 6 (20) ◽  
pp. 1031-1032 ◽  
Author(s):  
Kathryn Senior
Keyword(s):  
Oral Vaccine ◽  
Vaccine Delivery

scholarly journals Retraction: In vitro osteoinduction of human mesenchymal stem cells in biomimetic surface modified titanium alloy implants

2014 ◽  
Vol 33 (1) ◽  
pp. 148-148
Author(s):  
Sonia SANTANDER ◽  
Clara ALCAINE ◽  
Jaber LYAHYAI ◽  
Maria Angeles PÉREZ ◽  
Clementina RODELLAR ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Titanium Alloy ◽  
Human Mesenchymal Stem Cells ◽  
Biomimetic Surface ◽  
Surface Modified

Surface Modification Of Polymers With Grafting And Coating Of Silane Hybrids And Their Bioactivity

1999 ◽  
Vol 576 ◽  
Author(s):  
Masaaki Kubo ◽  
Seisuke Takashima ◽  
Kanji Tsuru ◽  
Satoshi Hayakawa ◽  
Akiyoshi Osaka ◽  
...  
Keyword(s):  
Contact Angle ◽  
High Density Polyethylene ◽  
Body Fluid ◽  
Chemical Species ◽  
Hydrated Silica ◽  
Bioactive Materials ◽  
Poly Vinyl Chloride ◽  
Essential Chemical ◽  
Surface Modified

ABSTRACTHydrated silica rich Si-OH and Si-0- groups serve in a body environment as sites for nucleation of apatite, and are known as an essential chemical species for bioactive materials. Organic polymers having surface modified with the hydrated silica will show bioactivity: bone tissues grow toward the apatite layer and bond to materials. Thus MOPS-M (3-methacryloxypropyltrimethoxysilane) was grafted under emulsion polymerization procedure to high density polyethylene (HDPE), poly (vinyl chloride) (PVC) and polyamide (PA) substrates to examine in vitro deposition of apatite (bioactivity) after soaking in a simulated body fluid (Kokubo solution). Bioactivity was confirmed for the grafted PVC and PA substrates and discussed in terms of contact angle and relative amount of grafted silane molecules.

Surface Modification of PEOT/PBT Membrane with Silk Fibroin Anchoring and its Potential Application in Artificial Salivary Gland Construct

2012 ◽  
Vol 538-541 ◽  
pp. 52-59
Author(s):  
Jie Zhu ◽  
Ming Shi Li ◽  
Li Qun Wang ◽  
Xiao Lin Zhu
Keyword(s):  
Salivary Gland ◽  
Silk Fibroin ◽  
Potential Application ◽  
Contact Angles ◽  
Water Contact ◽  
Polar Groups ◽  
Poly Ethylene Oxide ◽  
Poly Ethylene ◽  
Surface Modified

We reported the preparation of surface modified poly (ethylene oxide terephthalate) - poly (butylene terephthalate) membrane by the method of silk fibroin anchoring, namely SF/(PEOT/PBT). Its surface properties were characterized by contact angles and XPS and the biocompatibility of the composite membrane was further evaluated by human salivary epithelial cells (HSG cells) growth in vitro. Results revealed that SF/(PEOT/PBT) possessed the low water contact angle (48.0±3.0°) and immobilized a great amount of fibroin (fibroin surface coverage: 26.39 wt%), which attributed to the formation of polar groups such as hydrosulfide group, sulfonic group, carboxyl and carbonyl ones in the process of SO2 plasma treatment. HSG cells growth in vitro indicated that the silk fibroin anchoring could significantly enhance the biocompatibility of PEOT/PBT membrane, which suggested the potential application of fibroin anchoring PEOT/PBT for clinical HSG cells transplantation in the artificial salivary gland construct.

M-cell Targeting Acid-resistant Oral Vaccine Delivery for Immunization Against Hepatitis B Infection Using Cationic Solid Lipid Nanoparticles

2021 ◽  
Author(s):  
Surendra Saraf ◽  
Rudra Narayan Sahoo ◽  
Shailesh Jain ◽  
Subrata Mallick
Keyword(s):  
Hepatitis B ◽  
Solid Lipid Nanoparticles ◽  
Oral Vaccine ◽  
Vaccine Delivery ◽  
Lipid Nanoparticles ◽  
Hepatitis B Infection ◽  
Cell Targeting ◽  
M Cell ◽  
Promising Alternative ◽  
Solid Lipid

Abstract Background: Viral infection caused by Hepatitis B is transmitted by permucosal or parenteral exposure and also one of the prime causes of hepatocellular carcinoma and liver cirrhosis. Objectives: M-cell targeting acid-resistant oral vaccine delivery have been formulated for immunization against Hepatitis B infection. Methods: Cationic solid lipid nanoparticles (cSLNs) were prepared utilizing solvent injection technique. Hepatitis B surface antigen (HBsAg) loaded alginate coated cSLNs were anchored with lipopolysaccharide (LPS). SDS-PAGE was performed to evaluate acid degradation protection of prepared formulation. Induction of immunity produced by prepared nanoparticle for Hepatitis B was determined on female Balb/c mice followed by ELISA assays for assessing anti-HBsAg IgG/IgA antibodies in mucosal fluids. Results: Sustained release of HBsAg (60.66 %) has been exhibited from alginate coated cSLNs in comparison to cSLNs without alginate coating (97.72 %) after 48 h. The production of anti-HBs titer in intestinal, salivary and vaginal secretions was 3.41 IU/ml, 3.1 IU/ml and 2.51 IU/ml respectively in comparison to the control group. Integrity of the M-cells has been maintained after binding with SLN, and oral administration delivered the antigen to the desired site of gut. Conclusion: It was found effective in producing antibodies in mucosal immunization against Hepatitis B virus. So, this formulation could be used as a promising alternative preexisting vaccine to prevent Hepatitis B infection.

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