Wide-Field Multispectral Super-Resolution Imaging Using Spin-Dependent Fluorescence in Nanodiamonds

Nano Letters ◽  
2013 ◽  
Vol 13 (5) ◽  
pp. 2073-2077 ◽  
Author(s):  
Edward H. Chen ◽  
Ophir Gaathon ◽  
Matthew E. Trusheim ◽  
Dirk Englund
Keyword(s):  
Super Resolution ◽  
Wide Field ◽  
Resolution Imaging

scholarly journals Quantitative comparison of camera technologies for cost-effective Super-resolution Optical Fluctuation Imaging (SOFI)

2018 ◽  
Author(s):  
Robin Van den Eynde ◽  
Alice Sandmeyer ◽  
Wim Vandenberg ◽  
Sam Duwé ◽  
Wolfgang Hübner ◽  
...  
Keyword(s):  
Live Cell Imaging ◽  
Imaging System ◽  
Fluorescent Proteins ◽  
Imaging Techniques ◽  
Super Resolution ◽  
Cost Effective ◽  
Wide Field ◽  
Cmos Camera ◽  
Resolution Imaging ◽  
Cost Efficient

AbstractSuper-Resolution (SR) fluorescence microscopy is typically carried out on high-end research microscopes. Super-resolution Optical Fluctuation Imaging (SOFI) is a fast SR technique capable of live-cell imaging, that is compatible with many wide-field microscope systems. However, especially when employing fluorescent proteins, a key part of the imaging system is a very sensitive and well calibrated camera sensor. The substantial costs of such systems preclude many research groups from employing super-resolution imaging techniques.Here, we examine to what extent SOFI can be performed using a range of imaging hardware comprising different technologies and costs. In particular, we quantitatively compare the performance of an industry-grade CMOS camera to both state-of-the-art emCCD and sCMOS detectors, with SOFI-specific metrics. We show that SOFI data can be obtained using a cost-efficient industry-grade sensor, both on commercial and home-built microscope systems, though our analysis also readily exposes the merits of the per-pixel corrections performed in scientific cameras.

scholarly journals Autophagy-Related Proteins GABARAP and LC3B Label Structures of Similar Size but Different Shape in Super-Resolution Imaging

Molecules ◽  
2019 ◽  
Vol 24 (9) ◽  
pp. 1833 ◽  
Author(s):  
Iman Abdollahzadeh ◽  
Johnny Hendriks ◽  
Julia L. Sanwald ◽  
Indra M. Simons ◽  
Silke Hoffmann ◽  
...  
Keyword(s):  
Single Molecule ◽  
Super Resolution ◽  
Vesicular Trafficking ◽  
Hek293 Cells ◽  
Wide Field ◽  
Size Distributions ◽  
Similar Shape ◽  
Resolution Imaging ◽  
Localisation Microscopy ◽  
Related Proteins

Subcellular structures containing autophagy-related proteins of the Atg8 protein family have been investigated with conventional wide-field fluorescence and single molecule localisation microscopy. Fusion proteins of GABARAP and LC3B, respectively, with EYFP were overexpressed in HEK293 cells. While size distributions of structures labelled by the two proteins were found to be similar, shape distributions appeared quite disparate, with EYFP-GABARAP favouring circular structures and elliptical structures being dominant for EYFP-LC3B. The latter also featured a nearly doubled fraction of U-shape structures. The experimental results point towards highly differential localisation of the two proteins, which appear to label structures representing distinct stages or even specific channels of vesicular trafficking pathways. Our data also demonstrate that the application of super-resolution techniques expands the possibilities of fluorescence-based methods in autophagy studies and in some cases can rectify conclusions obtained from conventional fluorescence microscopy with diffraction-limited resolution.

Localized plasmon assisted structured illumination microscopy for wide-field high-speed dispersion-independent super resolution imaging

Nanoscale ◽  
2014 ◽  
Vol 6 (11) ◽  
pp. 5807-5812 ◽  
Author(s):  
Joseph Louis Ponsetto ◽  
Feifei Wei ◽  
Zhaowei Liu
Keyword(s):  
Antenna Array ◽  
High Speed ◽  
Super Resolution ◽  
Fluorescent Imaging ◽  
Wide Field ◽  
Structured Illumination ◽  
Structured Illumination Microscopy ◽  
Resolution Imaging ◽  
Localized Plasmon ◽  
Imaging Resolution

Fluorescent imaging resolution down to 51 nm is shown by generating tunable localized plasmon excitations on a nano-antenna array.

scholarly journals Wide-field multispectral super-resolution imaging using spin-dependent fluorescence in nanodiamonds

CLEO: 2013 ◽  
2013 ◽  
Author(s):  
Edward H. Chen ◽  
Ophir Gaathon ◽  
Matthew E. Trusheim ◽  
Dirk R. Englund
Keyword(s):  
Super Resolution ◽  
Wide Field ◽  
Resolution Imaging

scholarly journals Quantitative profiling of CD13 on single acute myeloid leukemia cells by super-resolution imaging and its implication in targeted drug susceptibility assessment

Nanoscale ◽  
2019 ◽  
Vol 11 (4) ◽  
pp. 1737-1744 ◽  
Author(s):  
Yan Xi ◽  
Dianbing Wang ◽  
Tingting Wang ◽  
Lin Huang ◽  
Xian-En Zhang
Keyword(s):  
Myeloid Leukemia ◽  
Super Resolution ◽  
Drug Susceptibility ◽  
Leukemia Cells ◽  
Wide Field ◽  
Susceptibility Assessment ◽  
Acute Myeloid Leukemia Cells ◽  
Resolution Imaging ◽  
Targeted Drug ◽  
Quantitative Profiling

Single particle protein on the AML cell membrane. Wide field image (left); SIM original image (middle); SIM 3D-reconstuction image (right).

scholarly journals Disordered Nanocomposite Islands for Nanospeckle Illumination Microscopy in Wide‐Field Super‐Resolution Imaging (Advanced Optical Materials 15/2021)

2021 ◽  
Vol 9 (15) ◽  
pp. 2170058
Author(s):  
Hajun Yoo ◽  
Hongki Lee ◽  
Woo Joong Rhee ◽  
Gwiyeong Moon ◽  
Changhun Lee ◽  
...  
Keyword(s):  
Optical Materials ◽  
Super Resolution ◽  
Wide Field ◽  
Resolution Imaging

scholarly journals Wide field of view 3D label-free super-resolution imaging

2018 ◽  
Author(s):  
Göran Maconi ◽  
Ivo Laidmäe ◽  
Ivan Kassamakov ◽  
Anton Nolvi ◽  
Jyrki Heinämäki ◽  
...  
Keyword(s):  
Super Resolution ◽  
Field Of View ◽  
Wide Field ◽  
Label Free ◽  
Wide Field Of View ◽  
Resolution Imaging

scholarly journals Gradient Permittivity Meta-Structure model for Wide-field Super-resolution imaging with a sub-45 nm resolution

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Shun Cao ◽  
Taisheng Wang ◽  
Wenbin Xu ◽  
Hua Liu ◽  
Hongxin Zhang ◽  
...  
Keyword(s):  
Super Resolution ◽  
Wide Field ◽  
Structure Model ◽  
Resolution Imaging
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