High-Flexibility Control of Structured Light with Combined Adaptive Optical Systems

Combining the specific advantages of high-resolution liquid-crystal-on-silicon spatial light modulators (LCoS-SLMs) and reflective or refractive micro-electro-mechanical systems (MEMS) presents new prospects for the generation of structured light fields. In particular, adaptive self-apodization schemes can significantly reduce diffraction by low-loss spatial filtering. The concept enables one to realize low-dispersion shaping of nondiffracting femtosecond wavepackets and to temporally switch, modulate or deflect spatially structured beams. Adaptive diffraction management by structured illumination is demonstrated for piezo-based and thermally actuated axicons, spiral phase plates (SPPs) and Fresnel bi-mirrors. Improved non-collinear autocorrelation with angular-tunable Fresnel-bi-mirrors via self-apodized illumination and phase contrast of an SLM is proposed. An extension of the recently introduced nondiffractive Talbot effect to a tunable configuration by combining an SLM and a fluid lens is reported. Experimental results for hexagonal as well as orthogonal array beams are presented.

Recent Progress in the Correlative Structured Illumination Microscopy

The super-resolution imaging technique of structured illumination microscopy (SIM) enables the mixing of high-frequency information into the optical transmission domain via light-source modulation, thus breaking the optical diffraction limit. Correlative SIM, which combines other techniques with SIM, offers more versatility or higher imaging resolution than traditional SIM. In this review, we first briefly introduce the imaging mechanism and development trends of conventional SIM. Then, the principles and recent developments of correlative SIM techniques are reviewed. Finally, the future development directions of SIM and its correlative microscopies are presented.

Miniature Structured Illumination Microscope for in vivo 3D Imaging of Brain Structures with Optical Sectioning

We present a high-resolution miniature, light-weight fluorescence microscope with electrowetting lens and onboard CMOS for high resolution volumetric imaging and structured illumination for rejection of out-of-focus and scattered light. The miniature microscope (SIMscope3D) delivers structured light using a coherent fiber bundle to obtain optical sectioning with an axial resolution of 18 μm. Volumetric imaging of eGFP labeled cells in fixed mouse brain tissue at depths up to 220 μm is demonstrated. The functionality of SIMscope3D to provide background free 3D imaging is shown by recording time series of microglia dynamics in awake mice at depths up to 120 μm in the brain.