Diversity Through Empowerment and Advocacy at the University of Wisconsin--Milwaukee

2014 ◽  
Author(s):  
Arpita Ghosh ◽  
Mark Charles
Author(s):  
Hans Ris

The High Voltage Electron Microscope Laboratory at the University of Wisconsin has been in operation a little over one year. I would like to give a progress report about our experience with this new technique. The achievement of good resolution with thick specimens has been mainly exploited so far. A cold stage which will allow us to look at frozen specimens and a hydration stage are now being installed in our microscope. This will soon make it possible to study undehydrated specimens, a particularly exciting application of the high voltage microscope.Some of the problems studied at the Madison facility are: Structure of kinetoplast and flagella in trypanosomes (J. Paulin, U. of Georgia); growth cones of nerve fibers (R. Hannah, U. of Georgia Medical School); spiny dendrites in cerebellum of mouse (Scott and Guillery, Anatomy, U. of Wis.); spindle of baker's yeast (Joan Peterson, Madison) spindle of Haemanthus (A. Bajer, U. of Oregon, Eugene) chromosome structure (Hans Ris, U. of Wisconsin, Madison). Dr. Paulin and Dr. Hanna are reporting their work separately at this meeting and I shall therefore not discuss it here.


Author(s):  
Patricia N. Hackney

Ustilago hordei and Ustilago violacea are yeast-like basidiomycete pathogens ofHordeum vulgare and Silene alba respectively. The mating type system in both species of Ustilago is bipolar, with alleles, A,a, (U.hordei) and a1, a2 (U.violacea) at a single locus. Haploid sporidia maintain the asexual phase by budding, while the sexual phase is initiated by conjugation tube formation between the mating types during budding and conjugation.For observation of budding, sporidia were prepared by culturing the four types on YEG (yeast extract glucose) broth for 24 hours. After centrifugation at 5000g cells were either left unmated or mated in a1/a2,A/a combinations. The sporidia were then mixed 1:1 with 4% agar and the resulting 1mm cubes fixed in 8% gluteraldehyde and post fixed in osmium tetroxide. After dehydration and embedding cubes were thin sectioned with a LKB ultratome and photographed in a Zeiss 9s transmission electron microscope or in an AE1 electron microscope of MK11 1MEV at the High Voltage Electron Microscopy Center of the University of Wisconsin-Madison.


1923 ◽  
Vol 9 (1) ◽  
pp. 81-90
Author(s):  
W. L. Westermann ◽  
A. G. Laird

1987 ◽  
Vol 3 (12) ◽  
pp. 337-348
Author(s):  
Robert Skloot

One of the ways in which Jews and others have sought somehow to assimilate the knowledge of the Nazi Holocaust has been through the theatrical expression of the appalling dilemmas it posed. Implicitly or explicitly, however, the process of ‘shaping’ that this involves forces an attitude to be taken by the dramatist towards the meaning of ‘choice’ in such circumstances, and the ‘acceptable’ price of possible survival. In his anthology The Theatre of the Holocaust (1982), Robert Skloot assembled four plays which exemplified the possible ‘attitudes to survival’, and here he relates them to the ideas of Bruno Bettelheim, Terrence Des Pres, and other writers on the subject, in an attempt to assess how fully and honestly theatre is able to reflect the issues involved. Robert Skloot is Professor of Theatre and Drama at the University of Wisconsin, Madison, and was Fulbright Lecturer in Israel in 1980–81. He has also edited a collection of essays, ‘The Darkness We Carry’: the Drama of the Holocaust, due for publication in the spring of 1988.


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