Effects of Melandrium firmum methanolic extract on testosterone-induced benign prostatic hyperplasia in Wistar rats

Abstract Background Benign prostatic hyperplasia (BPH) is a common disease which causes various health problems for elderly men such as urinary retention, recurring urinary tract infection and bladder stones. The aim of this study is to evaluate the therapeutic effects of Juniperus communis L. seed extract (JCS) on BPH in male Wistar rats. Methods To this end, 30 rats were divided into 5 groups (N = 6): group 1 (vehicle), group 2 (disease control), group 3 (standard medicine; 10 mg/kg finasteride), and groups 4 and 5 were treated with 300 mg/kg and 600 mg/kg of the hydroalcoholic JCS seed extract, respectively. Groups 2, 3, 4 and 5 received testosterone enanthate to induce prostatic hyperplasia. At the end of experimental period (28 days), prostate glands were cut off under anesthesia. Histopathological examination was done and biochemical parameters such as Malondialdehyde, Glutathione and protein carbonyl were also measured. Their body weights were also observed during the study. At the end of the experiment, prostate weights and prostate specific antigen (PSA) levels were measured. Prostate index, inhibition prostate weight and inhibition prostate index were also calculated. Results Both histopathological examination and biochemical parameter results showed significant improvements in rats treated with finasteride and 600 mg/kg JCS extract (p < 0.01). In addition, PSA levels showed significant decrease in comparison with the disease group. But acute toxicity test indicated that using JCS extract resulted in an increase in liver enzymes (ALP, LDH, SGOT, SGPT). As a result, the extract should be used with caution. Conclusions Oral administration of JCS extract is effective on preventing testosterone-induced benign prostatic hyperplasia.

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Cell-Free DNA Plasma Levels Differ in Age-Specific Pattern in Healthy Rats and Castrates with Testosterone-Induced Benign Prostatic Hyperplasia

International Journal of Genomics ◽

10.1155/2019/8173630 ◽

2019 ◽

Vol 2019 ◽

pp. 1-6 ◽

Cited By ~ 1

Author(s):

I. N. Vasilyeva ◽

V. G. Bespalov ◽

J. D. Von ◽

A. L. Semenov ◽

G. V. Tochilnikov ◽

...

Keyword(s):

Benign Prostatic Hyperplasia ◽

Wistar Rats ◽

Specific Pattern ◽

Prostatic Hyperplasia ◽

The Body ◽

Cell Free Dna ◽

Young Rats ◽

Free Dna ◽

Male Wistar Rats ◽

Old Rats

The purpose of this work was to study changes in the level of cell-free DNA (cfDNA) in the blood of young and old rats in the normal state and with induced benign prostatic hyperplasia (BPH). Male Wistar rats were divided into 4 groups—young (3 months), old (20 months), intact, or with testosterone-induced BPH. Groups with BPH were subjected to surgical castration and administration of testosterone esters at a dose of 25 mg/kg for a total of 7 injections for 20 days. In intact animals, the level of cfDNA in old rats (2.00±0.14 ng/μl) was significantly higher than that in the young (1.02±0.30 ng/μl). The body and the prostate weights of old rats were 1.6 and 1.4 times larger than those of the young, without an increase in the prostate index (PI). The testosterone level in the blood of young rats was 1.6 times higher than that of old (6.20±0.93 nmol/l vs. 3.77±0.55 nmol/l; NS). In animals with BPH, the level of cfDNA in old rats (3.14±0.76 ng/μl) was significantly higher than that in young rats (0.80±0.14 ng/μl). The body and the prostate weights in old rats were 1.8 and 2.3 times larger, than those in young rats, with an increase in the PI. The level of testosterone in the blood of young (15.76±0.51 nmol/l) and old (16.99±1.1 nmol/l) rats was not significantly different. Morphological signs of BPH were observed in the prostate of both young and old rats. During the induction of BPH in the experiment, according to the level of cfDNA, cell death processes have not changed significantly in young rats but significantly increased in old rats. A similar trend was observed in the group of intact animals. The obtained data indicate that apoptosis processes are enhanced during the development of BPH despite the growth of tissues in the prostate itself.

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Histostereological Restorative and Inhibitory Effects of Crude Methanolic Bark Extract of Prunus Africanus on Testosterone-Induced Benign Prostatic Hyperplasia in Wistar Rats

European Journal of Medicinal Plants ◽

10.9734/ejmp/2021/v32i1030424 ◽

2021 ◽

pp. 49-61

Author(s):

Kanyoni J. Mwangi ◽

Kweri J. Kariuki ◽

Mwangi A. Warimu ◽

Bett S. Kipyegon ◽

Sigei C. Chepngeno ◽

...

Keyword(s):

Benign Prostatic Hyperplasia ◽

Wistar Rats ◽

Prostate Volume ◽

Dose Range ◽

Study Groups ◽

Prostatic Hyperplasia ◽

Bark Extract ◽

Inhibitory Effects ◽

Group A ◽

Dose Dependent

Aim: To evaluate the histostereological effects of P. africanus on testosterone induced Benign Prostatic Hyperplasia (BPH) in Wistar rats. Place and Duration of Study: The study was carried out for six weeks in Jomo Kenyatta University of Agriculture and Technology. Methodology: A sample size of sixty Wistar were used as the experimental model and they were divided into two study groups of 30 rats in the restorative group and 30 rats in the inhibitory group. Each group was further categorized into 5 control and 25 experimental rats. The experimental rats were further subdivided into 5 sub-groups based on varying doses of the crude methanolic bark extract of P. africanus (0 mg, 25 mg, 50 mg, 125 mg, and 200 mg). BPH in experimental animal was induced by subcutaneous injection of testosterone propionate (7.5 mg/kg) for 10 days. Results: The findings of the study showed that the restorative group had a statistical significant (P<0.05) ((dose dependent reduction of the prostate volume, the stromal and epithelium volume was observed. While in the inhibitory group a statistical significant (P<0.05) dose-related inhibition, in the increase of the prostate volume, the stromal and epithelium volume was observed. In conclusion, the maximal restorative effect was observed to be up to 85.6% and at a dose of 200 mg, while the optimal inhibitory effects were observed to be between 66.7- 68.4% in the dose range of 100-200 mg/kg/body weight. Conclusion: Crude methanolic bark extract of P. africanus has both restorative.

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α-spinasterol from Melandrium firmum attenuates benign prostatic hyperplasia in a rat model

Molecular Medicine Reports ◽

10.3892/mmr.2014.2081 ◽

2014 ◽

Vol 9 (6) ◽

pp. 2362-2366 ◽

Cited By ~ 6

Author(s):

MEE-YOUNG LEE ◽

IN-SIK SHIN ◽

HWANGBO KYOUNG ◽

CHANG-SEOB SEO ◽

JONG-KEUN SON ◽

...

Keyword(s):

Benign Prostatic Hyperplasia ◽

Rat Model ◽

Prostatic Hyperplasia ◽

Melandrium Firmum

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Effect of Cassia fistula Hydroalcoholic Extract on Testosterone Induced Benign Prostatic Hyperplasia in Wistar Rats

Pharmacology and Toxicology of Natural Medicines (ISSN: 2756-6838) ◽

10.52406/ptnm.v1i1.9 ◽

2021 ◽

Vol 1 (1) ◽

pp. 14-19

Author(s):

Adesegun J. Kashimawo ◽

Kemelayefa O. James ◽

Nnabuike D. Nnamani

Keyword(s):

Free Radical ◽

Benign Prostatic Hyperplasia ◽

Wistar Rats ◽

Radical Scavenging Activity ◽

Prostate Gland ◽

Radical Scavenging ◽

Free Radical Scavenging ◽

Prostatic Hyperplasia ◽

Cassia Fistula ◽

Hydroalcoholic Extract

Background and Purpose: Men of age 40 years and above are at risk of non-cancerous enlargement of the prostate gland also known as benign prostatic hyperplasia (BPH). Adverse drug reactions and treatment relapse limit the effectiveness of orthodox pharmacotherapies. This study evaluated the effect of Cassia fistula hydroalcoholic extract on BPH. Methods: BPH was induced in Wistar rats by subcutaneous injection of 10 mg/kg/day of testosterone propionate (TP) for 7 days. The rats were randomly allotted to five groups: corn oil only; finasteride (FS) 5 mg/kg/day; and C. fistula extract at doses of 100, 200, and 400 mg/kg/day. A sixth group in which BPH was not induced received only the vehicle. At the end of 28 consecutive days of treatment, prostate and testicular weights and indices were evaluated. The in vitro antioxidant capacity of the extract was evaluated using the DPPH free radical scavenging method. Results: The extract showed a very strong free radical scavenging activity with IC50 value of 1.58 µg/mL (IC50 of gallic acid = 0.63 µg/mL) due to the presence of secondary metabolites. The results also showed significant (P?0.0001) reduction in the prostate weight, prostatic index, testes weight, and testes index of C. fistula extract-treated rats when compared with the untreated BPH group. Conclusion: These results suggest that C. fistula extract possesses potentials as a remedy for the treatment of BPH.

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Immunomodulatory Effect of Red Onion (Allium cepaLinn) Scale Extract on Experimentally Induced Atypical Prostatic Hyperplasia in Wistar Rats

Mediators of Inflammation ◽

10.1155/2014/640746 ◽

2014 ◽

Vol 2014 ◽

pp. 1-13 ◽

Cited By ~ 10

Author(s):

Ahmed A. Elberry ◽

Shagufta Mufti ◽

Jaudah Al-Maghrabi ◽

Essam Abdel Sattar ◽

Salah A. Ghareib ◽

...

Keyword(s):

Wistar Rats ◽

Cellular Proliferation ◽

Methanolic Extract ◽

Histopathological Examination ◽

Positive Control ◽

Prostatic Hyperplasia ◽

Saw Palmetto ◽

Hplc Profile ◽

Immune Enhancement ◽

Dose Dependent

Red onion scales (ROS) contain large amounts of flavonoids that are responsible for the reported antioxidant activity, immune enhancement, and anticancer property. Atypical prostatic hyperplasia (APH) was induced in adult castrated Wistar rats by both s.c. injection of testosterone (0.5 mg/rat/day) and by smearing citral on shaved skin once every 3 days for 30 days. Saw palmetto (100 mg/kg) as a positive control and ROS suspension at doses of 75, 150, and 300 mg/kg/day were given orally every day for 30 days. All medications were started 7 days after castration and along with testosterone and citral. The HPLC profile of ROS methanolic extract displayed two major peaks identified as quercetin and quercetin-4′-β-O-D-glucoside. Histopathological examination of APH-induced prostatic rats revealed evidence of hyperplasia and inflammation with cellular proliferation and reduced apoptosis Immunohistochemistry showed increased tissue expressions of IL-6, IL-8, TNF-α, IGF-1, and clusterin, while TGF-β1 was decreased, which correlates with the presence of inflammation. Both saw palmetto and RO scale treatment have ameliorated these changes. These ameliorative effects were more evident in RO scale groups and were dose dependent. In conclusion, methanolic extract of ROS showed a protective effect against APH induced rats that may be attributed to potential anti-inflammatory and immunomodulatory effects.

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