scholarly journals The deubiquitinating enzyme CYLD controls apical docking of basal bodies in ciliated epithelial cells

2014 ◽  
Vol 5 (1) ◽  
Author(s):  
Thibaut Eguether ◽  
Maria A. Ermolaeva ◽  
Yongge Zhao ◽  
Marion C. Bonnet ◽  
Ashish Jain ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Deubiquitinating Enzyme ◽  
Basal Bodies ◽  
Ciliated Epithelial Cells

scholarly journals Dishevelled controls apical docking and planar polarization of basal bodies in ciliated epithelial cells

2009 ◽  
Vol 331 (2) ◽  
pp. 486
Author(s):  
Philip B. Abitua ◽  
Tae Joo Park ◽  
Brian J. Mitchell ◽  
Chris Kintner ◽  
John B. Wallingford
Keyword(s):  
Epithelial Cells ◽  
Basal Bodies ◽  
Ciliated Epithelial Cells

scholarly journals Dishevelled controls apical docking and planar polarization of basal bodies in ciliated epithelial cells

2008 ◽  
Vol 40 (7) ◽  
pp. 871-879 ◽  
Author(s):  
Tae Joo Park ◽  
Brian J Mitchell ◽  
Philip B Abitua ◽  
Chris Kintner ◽  
John B Wallingford
Keyword(s):  
Epithelial Cells ◽  
Basal Bodies ◽  
Ciliated Epithelial Cells

scholarly journals Molecular characterization of centriole assembly in ciliated epithelial cells

2007 ◽  
Vol 178 (1) ◽  
pp. 31-42 ◽  
Author(s):  
Eszter K. Vladar ◽  
Tim Stearns
Keyword(s):  
Epithelial Cells ◽  
Mammalian Cells ◽  
Intraflagellar Transport ◽  
Proximal Region ◽  
Basal Bodies ◽  
Tracheal Epithelial Cells ◽  
Centriole Duplication ◽  
Centriole Assembly ◽  
Ciliated Epithelial Cells ◽  
Centrosomal Proteins

Ciliated epithelial cells have the unique ability to generate hundreds of centrioles during differentiation. We used centrosomal proteins as molecular markers in cultured mouse tracheal epithelial cells to understand this process. Most centrosomal proteins were up-regulated early in ciliogenesis, initially appearing in cytoplasmic foci and then incorporated into centrioles. Three candidate proteins were further characterized. The centrosomal component SAS-6 localized to basal bodies and the proximal region of the ciliary axoneme, and depletion of SAS-6 prevented centriole assembly. The intraflagellar transport component polaris localized to nascent centrioles before incorporation into cilia, and depletion of polaris blocked axoneme formation. The centriolar satellite component PCM-1 colocalized with centrosomal components in cytoplasmic granules surrounding nascent centrioles. Interfering with PCM-1 reduced the amount of centrosomal proteins at basal bodies but did not prevent centriole assembly. This system will help determine the mechanism of centriole formation in mammalian cells and how the limitation on centriole duplication is overcome in ciliated epithelial cells.

Shedding of Infected Ciliated Epithelial Cells in Rhinovirus Colds

1982 ◽  
Vol 145 (6) ◽  
pp. 849-853 ◽  
Author(s):  
R. B. Turner ◽  
J. O. Hendley ◽  
J. M. Gwaltney
Keyword(s):  
Epithelial Cells ◽  
Ciliated Epithelial Cells

scholarly journals Development of microtubule capping structures in ciliated epithelial cells

1987 ◽  
Vol 87 (1) ◽  
pp. 85-94
Author(s):  
R.W. Portman ◽  
E.L. LeCluyse ◽  
W.L. Dentler
Keyword(s):  
Epithelial Cells ◽  
Microtubule Assembly ◽  
Ciliated Epithelial Cells

Although capping structures are present at the tips of microtubules in both growing cilia and mature cilia, previous work has not determined the time of cap formation. The results reported here reveal that the large caps of mature palate cilia appear in cilia with lengths as short as 1.75 micron. In the growing palate cilium, a disk-shaped plate is formed at the tip during the first micron of growth. As the cilium elongates to 1.5-2.0 microns, a small plate forms underneath the disk-shaped plate that gives an asymmetrical appearance to the whole cap structure. The structure of the cap is complete in cilia longer than 2.0 microns. The hair-like structures that form the extraciliary crown appear on the membrane at the ciliary tip at the same time as the mature cap is forming. The formation of a cap structure is discussed in relation to microtubule assembly during ciliogenesis.

scholarly journals HNF-3/Forkhead Homologue-4 (HFH-4) Is Expressed in Ciliated Epithelial Cells in the Developing Mouse Lung

1999 ◽  
Vol 47 (6) ◽  
pp. 823-831 ◽  
Author(s):  
Jay W. Tichelaar ◽  
Susan E. Wert ◽  
Robert H. Costa ◽  
Shioko Kimura ◽  
Jeffrey A. Whitsett
Keyword(s):  
Epithelial Cells ◽  
Mouse Lung ◽  
Ciliated Epithelial Cells

scholarly journals STUDIES ON INFLUENZA INFECTION IN FERRETS BY MEANS OF FLUORESCEIN-LABELLED ANTIBODY

1955 ◽  
Vol 101 (6) ◽  
pp. 665-676 ◽  
Author(s):  
Ch'ien Liu
Keyword(s):  
Epithelial Cells ◽  
Influenza Infection ◽  
Bronchial Epithelium ◽  
Mediastinal Lymph Nodes ◽  
Viral Antigens ◽  
Antibody Staining ◽  
Hematoxylin And Eosin ◽  
Hematoxylin And Eosin Staining ◽  
Ciliated Epithelial Cells ◽  
Infectivity Titer

By means of fluorescein-labelled antibody staining, specific influenza viral antigens were seen in both the cytoplasm and the nucleus of infected ciliated epithelial cells covering the nasal turbinates of infected ferrets. Initially, only a small portion of the nasal epithelium showed fluorescence, and no appreciable abnormality of the cells could be detected by hematoxylin and eosin staining. The fluorescence soon spread to involve the entire epithelium, followed by desquamation coinciding with the onset of manifest illness. Pneumonia was seen in some of the infected ferrets, and in them, viral antigens were found in the bronchial epithelium and in the mediastinal lymph nodes. A rise of viral infectivity titer paralleled the observed spread of viral antigens. Many desquamated nasal epithelial cells and macrophages containing antigen were present in nasal smears. The finding would seem to offer a method for the rapid specific diagnosis of influenza infection.

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