scholarly journals Draft genome of the Native American cold hardy grapevine Vitis riparia Michx. ‘Manitoba 37’

2020 ◽  
Vol 7 (1) ◽  
Author(s):  
Sagar Patel ◽  
Michael Robben ◽  
Anne Fennell ◽  
Jason P. Londo ◽  
Dilmini Alahakoon ◽  
...  
2019 ◽  
Author(s):  
Daniela Holtgräwe ◽  
Thomas Rosleff Sörensen ◽  
Ludger Hausmann ◽  
Boas Pucker ◽  
Prisca Viehöver ◽  
...  

AbstractGrapevine breeding becomes highly relevant due to upcoming challenges like climate change, a decrease in the number of available fungicides, increasing public concern about plant protection, and the demand for a sustainable production. Downy mildew caused by Plasmopara viticola is one of the most devastating diseases worldwide of cultivated Vitis vinifera. Therefore, in modern breeding programs genetic marker technologies and genomic data are used to develop new cultivars with defined and stacked resistance loci. Potential sources of resistance are wild species of American or Asian origin. The interspecific hybrid of Vitis riparia Gm 183 x V. cinerea Arnold, available as the rootstock cultivar ‘Börner’, carries several relevant resistance loci. We applied next generation sequencing to enable the reliable identification of simple sequence repeats (SSR) and also generated a draft genome sequence assembly of ‘Börner’ to access genome wide sequence variations in a comprehensive and highly reliable way. These data were used to cover the ‘Börner’ genome with genetic marker positions. A subset of these marker positions was used for targeted mapping of the P. viticola resistance locus, Rpv14, to validate the marker position list. Based on the reference genome sequence PN40024, the position of this resistance locus can be narrowed down to less than 0.5 Mbp on chromosome 5.


Author(s):  
Diane Frome Loeb ◽  
Kathy Redbird

Abstract Purpose: In this article, we describe the existing literacy research with school-age children who are indigenous. The lack of data for this group of children requires speech-language pathologists (SLPs) to use expert opinion from indigenous and non-indigenous people to develop culturally sensitive methods for fostering literacy skills. Method: We describe two available curricula developed by indigenous people that are available, which use authentic materials and embed indigenous stories into the learning environment: The Indian Reading Series and the Northwest Native American Reading Curriculum. We also discuss the importance of using cooperative learning, multisensory instruction, and increased holistic emphasis to create a more culturally sensitive implementation of services. We provide an example of a literacy-based language facilitation that was developed for an indigenous tribe in Kansas. Conclusion: SLPs can provide services to indigenous children that foster literacy skills through storytelling using authentic materials as well as activities and methods that are consistent with the client's values and beliefs.


2002 ◽  
Author(s):  
Golda S. Ginsburg ◽  
John Walkup ◽  
Allison Barlow ◽  
Kristen Speakman

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