scholarly journals Proteome-wide and matrisome-specific alterations during human pancreas development and maturation

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Zihui Li ◽  
Daniel M. Tremmel ◽  
Fengfei Ma ◽  
Qinying Yu ◽  
Min Ma ◽  
...  
Keyword(s):  
Quantitative Proteomics ◽  
Developmental Stages ◽  
Age Groups ◽  
Immunofluorescent Staining ◽  
Pancreas Development ◽  
Human Pancreas ◽  
Proteomics Analysis ◽  
Ecm Proteins ◽  
Isobaric Tags ◽  
And Function

AbstractThe extracellular matrix (ECM) is unique to each tissue and capable of guiding cell differentiation, migration, morphology, and function. The ECM proteome of different developmental stages has not been systematically studied in the human pancreas. In this study, we apply mass spectrometry-based quantitative proteomics strategies using N,N-dimethyl leucine isobaric tags to delineate proteome-wide and ECM-specific alterations in four age groups: fetal (18-20 weeks gestation), juvenile (5-16 years old), young adults (21-29 years old) and older adults (50-61 years old). We identify 3,523 proteins including 185 ECM proteins and quantify 117 of them. We detect previously unknown proteome and matrisome features during pancreas development and maturation. We also visualize specific ECM proteins of interest using immunofluorescent staining and investigate changes in ECM localization within islet or acinar compartments. This comprehensive proteomics analysis contributes to an improved understanding of the critical roles that ECM plays throughout human pancreas development and maturation.

Children's individual approaches to the organization of narrative

1997 ◽  
Vol 24 (2) ◽  
pp. 279-309 ◽  
Author(s):  
GILLIAN WIGGLESWORTH
Keyword(s):  
Developmental Stages ◽  
Picture Book ◽  
Age Groups ◽  
The Subject ◽  
Similarities And Differences ◽  
Pass Through ◽  
And Function ◽  
The Way

This paper investigates the similarities and differences observed in individual approaches to the linguistic organization of narrative. Twenty subjects in each of five age groups (four, six, eight, ten years and adult) were asked to relate a narrative elicited from a picture book. All references to the animate characters in the book were coded for form (nominal/pronominal), and function (switch versus maintenance). Individual analyses of the narratives indicated that a variety of strategies were used across all age groups. Strategies identified included thematic subject, nominal and anaphoric. When the narrative was divided into segments based on the referential load of each segment, it was found that there was an interaction between the strategy adopted in the first segment, the age of the subject and the referential load of subsequent segments. A variety of strategies was adopted by all age groups although there were preferential trends observable within each group. The ability to maintain a strategy across the varying referential load of the narrative increased with age. Five developmental stages were identified from the analysis which enabled certain tentative predictions to be made about the way children approach a complex narrative task, suggesting that children pass through a number of stages which reflect their ability to organize the referential content of the narrative at differing speech levels.

scholarly journals Time-Resolved Extracellular Matrix Atlas of the Developing Human Skin Dermis

2021 ◽  
Vol 9 ◽  
Author(s):  
Mansheng Li ◽  
Xiao Li ◽  
Binghui Liu ◽  
Luye Lv ◽  
Wenjuan Wang ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Stem Cell ◽  
Age Groups ◽  
Skin Aging ◽  
Epidermal Stem Cell ◽  
Dermal Matrix ◽  
Time Resolved ◽  
Skin Development ◽  
Ecm Proteins ◽  
And Function

Skin aging is a physiological issue that is still relatively poorly understood. Studies have demonstrated that the dermal extracellular matrix (ECM) plays important roles in skin aging. However, the roles of the changes in ECM characteristics and the molecules that are secreted to the extracellular space and are involved in the formation of the dermal matrix from birth to old age remain unclear. To explore the way in which the ECM microenvironment supports the functions of skin development across different age groups is also poorly understood, we used a decellularization method and matrisome analysis to compare the composition, expression, and function of the dermal ECM in toddler, teenager, adult, and elderly skin. We found that the collagens, glycoproteins, proteoglycans, and regulatory factors that support skin development and interact with these core ECM proteins were differentially expressed at different ages. ECM expression markers occurring during the process of skin development were identified. In addition, our results elucidated the characteristics of ECM synthesis, response to skin development, and the features of the ECM that support epidermal stem cell growth via the basement membrane during skin aging.

scholarly journals Chronic marijuana usage by human pancreas donors is associated with impaired islet function

PLoS ONE ◽  
2021 ◽  
Vol 16 (10) ◽  
pp. e0258434
Author(s):  
Meirigeng Qi ◽  
John S. Kaddis ◽  
Kuan-Tsen Chen ◽  
Jeffrey Rawson ◽  
Keiko Omori ◽  
...  
Keyword(s):  
High Glucose ◽  
Cannabinoid Receptor ◽  
Marijuana Use ◽  
Human Islets ◽  
Immunofluorescent Staining ◽  
Human Pancreas ◽  
And Function

We investigated the effect of chronic marijuana use, defined as 4 times weekly for more than 3 years, on human pancreatic islets. Pancreata from deceased donors who chronically used marijuana were compared to those from age, sex and ethnicity matched non-users. The islets from marijuana-users displayed reduced insulin secretion as compared to islets from non-users upon stimulation with high glucose (AUC, 3.41 ± 0.62 versus 5.14 ±0.47, p<0.05) and high glucose plus KCl (AUC, 4.48 ± 0.41 versus 7.69 ± 0.58, p<0.001). When human islets from chronic marijuana-users were transplanted into diabetic mice, the mean reversal rate of diabetes was 35% versus 77% in animals receiving islets from non-users (p<0.01). Immunofluorescent staining for cannabinoid receptor type 1 (CB1R) was shown to be colocalized with insulin and enhanced significantly in beta cells from marijuana-users vs. non-users (CB1R intensity/islet area, 14.95 ± 2.71 vs. 3.23 ± 0.87, p<0.001). In contrast, CB1R expression was not co-localized with glucagon or somatostatin. Furthermore, isolated islets from chronic marijuana-users appeared hypertrophic. In conclusion, excessive marijuana use affects islet endocrine phenotype and function in vitro and in vivo. Given the increasing use of marijuana, our results underline the importance of including lifestyle when evaluating human islets for transplantation or research.

Quantitative Proteomics Analysis Reveals Unique But Overlapping Protein Signatures in HIV Infection

2019 ◽  
Author(s):  
Maha Al-Mozaini ◽  
Ibtihag S. Alsharif ◽  
Al-Hussain J. Alzahrani ◽  
Zakia Shinwari ◽  
Magid Halim ◽  
...  
Keyword(s):  
Hiv Infection ◽  
Quantitative Proteomics ◽  
Proteomics Analysis ◽  
Protein Signatures

scholarly journals Quantitative Proteomics Analysis Reveals Unique but Overlapping Protein Signatures in HIV Infections

2021 ◽  
Author(s):  
Maha Al-Mozaini ◽  
Ibtihag Alsharif ◽  
Alhusain Alzahrani ◽  
Zakia Shinwari ◽  
Magid Halim ◽  
...  
Keyword(s):  
Quantitative Proteomics ◽  
Hiv Infections ◽  
Proteomics Analysis ◽  
Protein Signatures

scholarly journals Mitochondrial Processes during Early Development of Dictyostelium discoideum: From Bioenergetic to Proteomic Studies

Genes ◽  
2021 ◽  
Vol 12 (5) ◽  
pp. 638
Author(s):  
Monika Mazur ◽  
Daria Wojciechowska ◽  
Ewa Sitkiewicz ◽  
Agata Malinowska ◽  
Bianka Świderska ◽  
...  
Keyword(s):  
Life Cycle ◽  
Developmental Stages ◽  
Coupling Efficiency ◽  
Slime Mold ◽  
Intact Cells ◽  
Life Cycle Stages ◽  
Early Developmental Stages ◽  
Proteomic Study ◽  
And Function ◽  
Early Developmental

The slime mold Dictyostelium discoideum’s life cycle includes different unicellular and multicellular stages that provide a convenient model for research concerning intracellular and intercellular mechanisms influencing mitochondria’s structure and function. We aim to determine the differences between the mitochondria isolated from the slime mold regarding its early developmental stages induced by starvation, namely the unicellular (U), aggregation (A) and streams (S) stages, at the bioenergetic and proteome levels. We measured the oxygen consumption of intact cells using the Clarke electrode and observed a distinct decrease in mitochondrial coupling capacity for stage S cells and a decrease in mitochondrial coupling efficiency for stage A and S cells. We also found changes in spare respiratory capacity. We performed a wide comparative proteomic study. During the transition from the unicellular stage to the multicellular stage, important proteomic differences occurred in stages A and S relating to the proteins of the main mitochondrial functional groups, showing characteristic tendencies that could be associated with their ongoing adaptation to starvation following cell reprogramming during the switch to gluconeogenesis. We suggest that the main mitochondrial processes are downregulated during the early developmental stages, although this needs to be verified by extending analogous studies to the next slime mold life cycle stages.

scholarly journals MFng Is Dispensable for Mouse Pancreas Development and Function

2009 ◽  
Vol 29 (8) ◽  
pp. 2129-2138 ◽  
Author(s):  
Per Svensson ◽  
Ingela Bergqvist ◽  
Stefan Norlin ◽  
Helena Edlund
Keyword(s):  
Cell Differentiation ◽  
Notch Signaling ◽  
Pancreas Development ◽  
Loss Of Function ◽  
Pancreatic Cell ◽  
Targeted Deletion ◽  
Mouse Pancreas ◽  
Pancreatic Progenitor ◽  
Pancreatic Progenitor Cells ◽  
And Function

ABSTRACT Notch signaling regulates pancreatic cell differentiation, and mutations of various Notch signaling components result in perturbed pancreas development. Members of the Fringe family of β1,3-N-acetylglucosaminyltransferases, Manic Fringe (MFng), Lunatic Fringe (LFng), and Radical Fringe (RFng), modulate Notch signaling, and MFng has been suggested to regulate pancreatic endocrine cell differentiation. We have characterized the expression of the three mouse Fringe genes in the developing mouse pancreas between embryonic days 9 and 14 and show that the expression of MFng colocalized with the proendocrine transcription factor Ngn3. In contrast, the expression of LFng colocalized with the exocrine marker Ptf1a, whereas RFng was not expressed. Moreover, we show that expression of MFng is lost in Ngn3 mutant mice, providing evidence that MFng is genetically downstream of Ngn3. Gain- and loss-of-function analyses of MFng by the generation of mice that overexpress MFng in early pancreatic progenitor cells and mice with a targeted deletion of MFng provide, however, evidence that MFng is dispensable for pancreas development and function, since no pancreatic defects in these mice were observed.

A Gel-Free Quantitative Proteomics Analysis of Factors Released From Hypoxic-Conditioned Placentae

2009 ◽  
Vol 17 (3) ◽  
pp. 247-257 ◽  
Author(s):  
Richard T. Blankley ◽  
Nicola J. Robinson ◽  
John D. Aplin ◽  
Ian P. Crocker ◽  
Simon J. Gaskell ◽  
...  
Keyword(s):  
Quantitative Proteomics ◽  
Proteomics Analysis

scholarly journals Quantitative proteomics analysis reveals similar release profiles following specific PAR-1 or PAR-4 stimulation of platelets

2014 ◽  
Vol 103 (1) ◽  
pp. 140-146 ◽  
Author(s):  
Thijs C. van Holten ◽  
Onno B. Bleijerveld ◽  
Patrick Wijten ◽  
Philip G. de Groot ◽  
Albert J.R. Heck ◽  
...  
Keyword(s):  
Quantitative Proteomics ◽  
Proteomics Analysis ◽  
Stimulation Of ◽  
Release Profiles

scholarly journals Phylogenetic analysis of the caspase family in bivalves: implications for programmed cell death, immune response and development

BMC Genomics ◽  
2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Susanne Vogeler ◽  
Stefano Carboni ◽  
Xiaoxu Li ◽  
Alyssa Joyce
Keyword(s):  
Immune Response ◽  
Caspase 3 ◽  
Developmental Stages ◽  
Phylogenetic Analyses ◽  
Apoptotic Pathway ◽  
Caspase Family ◽  
Executioner Caspases ◽  
And Function ◽  
Relationship Of ◽  
Inflammatory Caspases

Abstract Background Apoptosis is an important process for an organism’s innate immune system to respond to pathogens, while also allowing for cell differentiation and other essential life functions. Caspases are one of the key protease enzymes involved in the apoptotic process, however there is currently a very limited understanding of bivalve caspase diversity and function. Results In this work, we investigated the presence of caspase homologues using a combination of bioinformatics and phylogenetic analyses. We blasted the Crassostrea gigas genome for caspase homologues and identified 35 potential homologues in the addition to the already cloned 23 bivalve caspases. As such, we present information about the phylogenetic relationship of all identified bivalve caspases in relation to their homology to well-established vertebrate and invertebrate caspases. Our results reveal unexpected novelty and complexity in the bivalve caspase family. Notably, we were unable to identify direct homologues to the initiator caspase-9, a key-caspase in the vertebrate apoptotic pathway, inflammatory caspases (caspase-1, − 4 or − 5) or executioner caspases-3, − 6, − 7. We also explored the fact that bivalves appear to possess several unique homologues to the initiator caspase groups − 2 and − 8. Large expansions of caspase-3 like homologues (caspase-3A-C), caspase-3/7 group and caspase-3/7-like homologues were also identified, suggesting unusual roles of caspases with direct implications for our understanding of immune response in relation to common bivalve diseases. Furthermore, we assessed the gene expression of two initiator (Cg2A, Cg8B) and four executioner caspases (Cg3A, Cg3B, Cg3C, Cg3/7) in C. gigas late-larval development and during metamorphosis, indicating that caspase expression varies across the different developmental stages. Conclusion Our analysis provides the first overview of caspases across different bivalve species with essential new insights into caspase diversity, knowledge that can be used for further investigations into immune response to pathogens or regulation of developmental processes.

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