scholarly journals Iron-mediated aggregation and toxicity in a novel neuronal cell culture model with inducible alpha-synuclein expression

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Martin Bartels ◽  
Daniel Weckbecker ◽  
Peer-Hendrik Kuhn ◽  
Sergey Ryazanov ◽  
Andrei Leonov ◽  
...  
Keyword(s):  
Cell Culture ◽  
Neuronal Cell ◽  
Alpha Synuclein ◽  
Cell Culture Model ◽  
Neuronal Cell Culture ◽  
Culture Model

scholarly journals Proteomics of the human neuronal cell culture model of Alzheimer's disease

IBRO Reports ◽  
2019 ◽  
Vol 6 ◽  
pp. S109
Author(s):  
Min-Young Song ◽  
Da Kyeong Park ◽  
Chaewon Park ◽  
Dain Kim ◽  
Soo Youn Lee ◽  
...  
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Cell Culture ◽  
Neuronal Cell ◽  
Cell Culture Model ◽  
Neuronal Cell Culture ◽  
Human Neuronal Cell ◽  
Model Of Alzheimer’S Disease ◽  
Culture Model

scholarly journals Alterations in mitochondrial dynamics induced by tebufenpyrad and pyridaben in a dopaminergic neuronal cell culture model

2016 ◽  
Vol 53 ◽  
pp. 302-313 ◽  
Author(s):  
Adhithiya Charli ◽  
Huajun Jin ◽  
Vellareddy Anantharam ◽  
Arthi Kanthasamy ◽  
Anumantha G. Kanthasamy
Keyword(s):  
Cell Culture ◽  
Mitochondrial Dynamics ◽  
Neuronal Cell ◽  
Cell Culture Model ◽  
Neuronal Cell Culture ◽  
Dopaminergic Neuronal Cell ◽  
Culture Model

Cerium Oxide Nanoparticles Prevent Nitrosative Stress in Neuronal Cell Culture Model

2010 ◽  
Vol 49 ◽  
pp. S181 ◽  
Author(s):  
Janet M Dowding ◽  
Sarah Lubitz ◽  
Ajay Karakoti ◽  
Andrew Kim ◽  
Sudipta Seal ◽  
...  
Keyword(s):  
Cell Culture ◽  
Cerium Oxide ◽  
Nitrosative Stress ◽  
Neuronal Cell ◽  
Cerium Oxide Nanoparticles ◽  
Oxide Nanoparticles ◽  
Cell Culture Model ◽  
Neuronal Cell Culture ◽  
Culture Model

Simulated Stroke in a Mouse Neuronal Cell Culture Model

2020 ◽  
Vol 34 (S1) ◽  
pp. 1-1
Author(s):  
Luise J. Meyer ◽  
Josephine Eskaf ◽  
Michele M. Salzman ◽  
Zhu Li ◽  
Matthias L. Riess
Keyword(s):  
Cell Culture ◽  
Neuronal Cell ◽  
Cell Culture Model ◽  
Neuronal Cell Culture ◽  
Culture Model

scholarly journals Contemporary circulating enterovirus D68 strains show differential viral entry and replication in human neuronal cells

2018 ◽  
Author(s):  
David M Brown ◽  
Alison M Hixon ◽  
Lauren M Oldfield ◽  
Yun Zhang ◽  
Mark Novotny ◽  
...  
Keyword(s):  
Cell Culture ◽  
Viral Replication ◽  
Cell Line ◽  
Viral Entry ◽  
Neuronal Cells ◽  
Neuronal Cell ◽  
Cell Culture Model ◽  
Acute Flaccid Myelitis ◽  
Culture Model ◽  
Enterovirus D68

AbstractHistorically, enterovirus D68 (EV-D68) has primarily been associated with respiratory illnesses. However, in the summers of 2014 and 2016 EV-D68 outbreaks coincided with a spike in polio-like acute flaccid myelitis/paralysis (AFM/AFP) cases. This raised concerns that the EV-D68 virus could be the causative agent of AFM during these recent outbreaks. To assess the neurotropic capacity of EV-D68, we explored the use of the neuroblastoma-derived neuronal cell line, SH-SY5Y, as a tissue culture model to determine if differential infection permissibility is observed for different EV-D68 strains. In contrast to HeLa and A549 cells, which support viral infection of all EV-D68 strains tested, SH-SY5Y cells only supported infection by a subset of contemporary EV-D68 strains, including members from the 2014 outbreak. Viral replication and infectivity in SH-SY5Y was assessed using four different assays – infectious virus production, cytopathic effects, cellular ATP release, and VP1 capsid protein production – with similar results. Similar differential neurotropism was also observed in differentiated SH-SY5Y cells, primary human neuron cultures, and a mouse paralysis model. Using the SH-SY5Y cell culture model, we determined that barriers to viral entry was at least partly responsible for the differential infectivity phenotype, since transfection of genomic RNA into SH-SY5Y generated virions for all EV-D68 isolates, but only a single round of replication was observed from strains which could not directly infect SH-SY5Y. In addition to supporting virus replication and other functional studies, this cell culture model may help confirm epidemiological associations between EV-D68 strains and AFM and allow for the rapid identification of emerging neurotropic strains.Author SummarySince the outbreak during the summer of 2014, EV-D68 has been linked to a type of limb paralysis referred to as acute flaccid myelitis (AFM), with evidence mounting for the causal link of EV-D68 to AFM. Among these AFM cases, concurrent EV-D68 infection was confirmed in several independent epidemiological clusters in four continents. In this report, we describe a neuronal cell culture model (SH-SY5Y cells) where only a subset of contemporary 2014 outbreak strains of EV-D68 show infectivity in neuronal cells, or neurotropism, based on four different assays of viral replication and infection. We further confirmed the observed difference in neurotropismin vitrousing primary human neuron cell cultures andin vivowith a mouse paralysis model. Using the SH-SY5Y cell model, we determined that a barrier to viral entry is at least partly responsible for neurotropism. SH-SY5Y cells may be useful in determining if specific EV-D68 genetic determinants are associated with neuropathogenesis, and replication in this cell line could be used as rapid screening tool for identifying neurotropic EV-D68 strains. This may assist with better understanding of pathogenesis and epidemiology, and with the development of potential therapies.

scholarly journals Contemporary Circulating Enterovirus D68 Strains Have Acquired the Capacity for Viral Entry and Replication in Human Neuronal Cells

mBio ◽  
2018 ◽  
Vol 9 (5) ◽  
Author(s):  
David M. Brown ◽  
Alison M. Hixon ◽  
Lauren M. Oldfield ◽  
Yun Zhang ◽  
Mark Novotny ◽  
...  
Keyword(s):  
Cell Culture ◽  
Cell Line ◽  
Viral Entry ◽  
Neuronal Cells ◽  
Neuronal Cell ◽  
Rapid Screening ◽  
Cell Culture Model ◽  
Culture Model ◽  
Enterovirus D68 ◽  
Human Neuron

ABSTRACTEnterovirus D68 (EV-D68) has historically been associated with respiratory illnesses. However, in the summers of 2014 and 2016, EV-D68 outbreaks coincided with a spike in polio-like acute flaccid myelitis/paralysis (AFM/AFP) cases. This raised concerns that EV-D68 could be the causative agent of AFM during these recent outbreaks. To assess the potential neurotropism of EV-D68, we utilized the neuroblastoma-derived neuronal cell line SH-SY5Y as a cell culture model to determine if differential infection is observed for different EV-D68 strains. In contrast to HeLa and A549 cells, which support viral infection of all EV-D68 strains tested, SH-SY5Y cells only supported infection by a subset of contemporary EV-D68 strains, including isolates from the 2014 outbreak. Viral replication and infectivity in SH-SY5Y were assessed using multiple assays: virus production, cytopathic effects, cellular ATP release, and VP1 capsid protein production. Similar differential neurotropism was also observed in differentiated SH-SY5Y cells, primary human neuron cultures, and a mouse paralysis model. Using the SH-SY5Y cell culture model, we determined that barriers to viral binding and entry were at least partly responsible for the differential infectivity phenotype. Transfection of genomic RNA into SH-SY5Y generated virions for all EV-D68 isolates, but only a single round of replication was observed from strains that could not directly infect SH-SY5Y. In addition to supporting virus replication and other functional studies, this cell culture model may help identify the signatures of virulence to confirm epidemiological associations between EV-D68 strains and AFM and allow for the rapid identification and characterization of emerging neurotropic strains.IMPORTANCESince the EV-D68 outbreak during the summer of 2014, evidence of a causal link to a type of limb paralysis (AFM) has been mounting. In this article, we describe a neuronal cell culture model (SH-SY5Y cells) in which a subset of contemporary 2014 outbreak strains of EV-D68 show infectivity in neuronal cells, or neurotropism. We confirmed the difference in neurotropismin vitrousing primary human neuron cell cultures andin vivowith a mouse paralysis model. Using the SH-SY5Y cell model, we determined that a barrier to viral entry is at least partly responsible for neurotropism. SH-SY5Y cells may be useful in determining if specific EV-D68 genetic determinants are associated with neuropathogenesis, and replication in this cell line could be used as rapid screening tool for identification of neurotropic EV-D68 strains. This may assist with better understanding of pathogenesis and epidemiology and with the development of potential therapies.

Protective Effect of Amyloid-β Peptides Against Herpes Simplex Virus-1 Infection in a Neuronal Cell Culture Model

2016 ◽  
Vol 50 (4) ◽  
pp. 1227-1241 ◽  
Author(s):  
Karine Bourgade ◽  
Aurélie Le Page ◽  
Christian Bocti ◽  
Jacek M. Witkowski ◽  
Gilles Dupuis ◽  
...  
Keyword(s):  
Cell Culture ◽  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Protective Effect ◽  
Amyloid Β ◽  
Neuronal Cell ◽  
Cell Culture Model ◽  
Herpes Simplex Virus 1 ◽  
Culture Model ◽  
Simplex Virus

1120: Citrate and Vitamin E Blunt the SWL-Induced Free Radical Surge in an In-Vitro MDCK Cell Culture Model

2004 ◽  
Vol 171 (4S) ◽  
pp. 295-295
Author(s):  
Fernando C. Delvecchio ◽  
Ricardo M. Brizuela ◽  
Karen J. Byer ◽  
W. Patrick Springhart ◽  
Saeed R. Khan ◽  
...  
Keyword(s):  
Cell Culture ◽  
Free Radical ◽  
Vitamin E ◽  
Mdck Cell ◽  
Cell Culture Model ◽  
Culture Model
Sign in / Sign up

Export Citation Format

Share Document