scholarly journals Optimization of heterotrophic cultivation of Chlorella sp. HS2 using screening, statistical assessment, and validation

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Hee Su Kim ◽  
Won-Kun Park ◽  
Bongsoo Lee ◽  
Gyeongho Seon ◽  
William I. Suh ◽  
...  
Keyword(s):  
Biomass Concentration ◽  
Biomass Productivity ◽  
Nitrogen Sources ◽  
Full Potential ◽  
Chlorella Sp ◽  
Bg11 Medium ◽  
Heterotrophic Cultivation ◽  
Dipotassium Phosphate ◽  
Burman Design ◽  
Major Nutrients

AbstractThe heterotrophic cultivation of microalgae has a number of notable advantages, which include allowing high culture density levels as well as enabling the production of biomass in consistent and predictable quantities. In this study, the full potential of Chlorella sp. HS2 is explored through optimization of the parameters for its heterotrophic cultivation. First, carbon and nitrogen sources were screened in PhotobioBox. Initial screening using the Plackett-Burman design (PBD) was then adopted and the concentrations of the major nutrients (glucose, sodium nitrate, and dipotassium phosphate) were optimized via response surface methodology (RSM) with a central composite design (CCD). Upon validation of the model via flask-scale cultivation, the optimized BG11 medium was found to result in a three-fold improvement in biomass amounts, from 5.85 to 18.13 g/L, in comparison to a non-optimized BG11 medium containing 72 g/L glucose. Scaling up the cultivation to a 5-L fermenter resulted in a greatly improved biomass concentration of 35.3 g/L owing to more efficient oxygenation of the culture. In addition, phosphorus feeding fermentation was employed in an effort to address early depletion of phosphate, and a maximum biomass concentration of 42.95 g/L was achieved, with biomass productivity of 5.37 g/L/D.

Effects of Temperature on the Growth and Product Accumulation of Chlorella sp.

2013 ◽  
Vol 712-715 ◽  
pp. 428-432 ◽  
Author(s):  
Bi Lin Lu ◽  
Liang Qi ◽  
Ming Xi Li
Keyword(s):  
Energy Security ◽  
Ghg Emissions ◽  
Transport Sector ◽  
Accumulation Process ◽  
Chlorella Sp ◽  
Bg11 Medium ◽  
Potential Source ◽  
Effects Of Temperature ◽  
Product Accumulation ◽  
Global Growth

In recent years, the use of liquid biofuels in the transport sector has shown rapid global growth, driven mostly by policies focused on achievement of energy security, and mitigation of GHG emissions. Microalgae, as biomass, are a potential source of renewable energy, and they can be converted into energy such as biofuel oil and gas.In this paper, the microalgae strain of Chlorella sp. was supplied in BG11 medium, and Cultivation optimization were experimented. The results were showed that temperature was an important factor which could influence growth and accumulation of secondary metabolite in the cell from accumulation process of biological products, temperature which was suitable for the growth of chlorella was 25°C.

Optimization of the decolorization conditions of Rose Bengal by using Aspergillus niger TF05 and a decolorization mechanism

Microbiology ◽  
2022 ◽  
Vol 168 (1) ◽  
Author(s):  
Minghui Zhou ◽  
Yan Zhang ◽  
Yajun Chen ◽  
Fangyan Zhang ◽  
Daihu Yang
Keyword(s):  
Aspergillus Niger ◽  
Ammonium Chloride ◽  
Rose Bengal ◽  
Metal Ion ◽  
Degradation Products ◽  
Uniform Design ◽  
Nitrogen Sources ◽  
Spore Concentration ◽  
Rose Bengal Dye ◽  
Burman Design

Aspergillus niger TF05 was applied to decolorize Rose Bengal dye. The effects of carbon source, nitrogen source, metal ion and spore concentration on Rose Bengal treatment with A. niger TF05 were studied. A Plackett–Burman design (PBD) and a uniform design (UD) were used to optimize the decolorization conditions of A. niger TF05 and enhance its decolorization effect. The mechanism of Rose Bengal decolorization by A. niger TF05 was examined by analysing degradation products via UV–visible light spectroscopy, IR spectroscopy and GC-MS. The best decolorization effect was achieved in the single factor test with glucose and ammonium chloride as carbon and nitrogen sources, respectively. Mg2+ was an essential ion that could improve the mould ball state and adsorption efficiency if the spore concentration was maintained at 106 spores ml–1. The optimal decolorization conditions obtained using the PBD and UD methods were 11.5 g l−1 glucose, 6.5 g l−1 ammonium chloride, 0.4 g l−1 magnesium sulphate, pH 5.8, 28 °C, 140 r.p.m. rotational speed, 0.18 g l−1 dye concentration, 0.5 ml of inocula and 120 h decolorization time. Under these conditions, the maximum decolorization rate was 106%. Spectral analysis suggested that the absorption peak of the product changed clearly after decolorization; GC-MS analysis revealed that the intermediate product tetrachlorophthalic anhydride formed after decolorization. The combined use of the PBD and UD methods can optimize multi-factor experiments. A. niger TF05 decolorized Rose Bengal during intracellular enzymatic degradation after adsorption.

scholarly journals The effect of the nitrogen source type on the growth and consumption of crude glycerol by Streptomyces hygroscopicus CH-7

2021 ◽  
Vol 10 (1) ◽  
pp. 41-45
Author(s):  
Slavica Ilić ◽  
Jovan Ćirić ◽  
Gordana Gojgić-Cvijović
Keyword(s):  
Amino Acids ◽  
Sunflower Oil ◽  
Crude Glycerol ◽  
Biomass Concentration ◽  
Nitrogen Sources ◽  
Biodiesel Production ◽  
Streptomyces Hygroscopicus ◽  
Basic Medium ◽  
Source Type ◽  
Glycerol Consumption

In this paper we studied the effect of different amino acids (arginine, tryptophan, tyrosine, and phenylalanine) as nitrogen sources on the growth of actinomycete Streptomyces hygroscopicus CH-7 and the consumption of crude glycerol, obtained as a by-product in the biodiesel production from sunflower oil. The highest biomass concentration (9.5 g/L) was achieved using the basic medium and the medium with tryptophan (9.2 g/L), while the crude glycerol consumption was the highest in the basic medium (5.9 mg/mL) and the medium with phenylalanine (3.3 mg/mL).

scholarly journals Influence of Light Intensity and Temperature on Cultivation of Microalgae Desmodesmus Communis in Flasks and Laboratory-Scale Stirred Tank Photobioreactor

2015 ◽  
Vol 52 (2) ◽  
pp. 59-70 ◽  
Author(s):  
J. Vanags ◽  
L. Kunga ◽  
K. Dubencovs ◽  
V. Galvanauskas ◽  
O. Grīgs
Keyword(s):  
Light Intensity ◽  
Shake Flask ◽  
Scale Up ◽  
Biomass Concentration ◽  
Biomass Productivity ◽  
Pilot Scale ◽  
Laboratory Scale ◽  
Stirred Tank ◽  
Light Limitation ◽  
Maximum Biomass

Abstract Optimization of the microalgae cultivation process and of the bioprocess in general traditionally starts with cultivation experiments in flasks. Then the scale-up follows, when the process from flasks is transferred into a laboratory-scale bioreactor, in which further experiments are performed before developing the process in a pilot-scale reactor. This research was done in order to scale-up the process from a 0.4 1 shake flask to a 4.0 1 laboratory-scale stirred-tank photobioreactor for the cultivation of Desmodesmus (D.) communis microalgae. First, the effect of variation in temperature (21-29 ºC) and in light intensity (200-600 μmol m-2s-1) was studied in the shake-flask experiments. It was shown that the best results (the maximum biomass concentration of 2.72 g 1-1 with a specific growth rate of 0.65 g g-1d-1) can be achieved at the cultivation temperature and light intensity being 25 °C and 300 μmol m2s-1, respectively. At the same time, D. communis cultivation under the same conditions in stirred-tank photobioreactor resulted in average volumetric productivities of biomass due to the light limitation even when the light intensity was increased during the experiment (the maximum biomass productivity 0.25 g 1-1d-1; the maximum biomass concentration 1.78 g 1-1).

Effects of different nitrogen sources and light paths of flat plate photobioreactors on the growth and lipid accumulation of Chlorella sp. GN1 outdoors

2020 ◽  
Vol 301 ◽  
pp. 122762 ◽  
Author(s):  
Pingzhong Feng ◽  
Zhongbin Xu ◽  
Lei Qin ◽  
Md Asraful Alam ◽  
Zhongming Wang ◽  
...  
Keyword(s):  
Flat Plate ◽  
Lipid Accumulation ◽  
Nitrogen Sources ◽  
Chlorella Sp

scholarly journals Medium Optimization for Spore Production of a Straw-Cellulose Degrading Actinomyces Strain under Solid-State Fermentation Using Response Surface Method

2020 ◽  
Vol 12 (21) ◽  
pp. 8893
Author(s):  
Huanran Liu ◽  
Dan Zhang ◽  
Xia Zhang ◽  
Chuanzhi Zhou ◽  
Pei Zhou ◽  
...  
Keyword(s):  
Solid State ◽  
Solid State Fermentation ◽  
Inorganic Nitrogen ◽  
Nitrogen Sources ◽  
Spore Production ◽  
Steepest Ascent ◽  
Medium Components ◽  
Burman Design ◽  
Plackett Burman Design ◽  
Central Composite

The strains capable of degrading cellulose have attracted much interest because of their applications in straw resource utilization in solid-state fermentation (SSF). However, achieving high spore production in SSF is rarely reported. The production of spores from Streptomyces griseorubens JSD-1 was investigated in shaker-flask cultivation in this study. The optimal carbon, organic nitrogen and inorganic nitrogen sources were sucrose, yeast extract and urea, respectively. Plackett–Burman design (PBD) was adopted to determine the key medium components, and the concentration levels of three components (urea, NaCl, MgSO4·7H2O) were optimized with the steepest ascent path and central composite design (CCD), achieving 1.72 × 109 CFU/g of spore production. Under the optimal conditions (urea 2.718% w/v, NaCl 0.0697% w/v, MgSO4·7H2O 0.06956% w/v), the practical value of spore production was 1.69 × 109 CFU/g. The determination coefficient (R2) was 0.9498, which ensures an adequate credibility of the model.

scholarly journals Successive Nonstatistical and Statistical Approaches for the Improved Antibiotic Activity of Rare ActinomyceteNonomuraeasp. JAJ18

2014 ◽  
Vol 2014 ◽  
pp. 1-11 ◽  
Author(s):  
Polpass Arul Jose ◽  
Solomon Robinson David Jebakumar
Keyword(s):  
Response Surface ◽  
Large Scale ◽  
Polynomial Regression ◽  
Antibiotic Production ◽  
Nitrogen Sources ◽  
Optimization Method ◽  
Antibiotic Activity ◽  
Media Optimization ◽  
Burman Design ◽  
Nutritional Constituents

The selection and optimization of nutritional constituents as well as their levels for the improved production of antibiotic byNonomuraeasp. JAJ18 were carried out using combination of both nonstatistical one-factor-at-a-time (OFAT) method and statistical response surface methodology (RSM). Using OFAT method, starch and (NH4)2SO4were identified as suitable carbon and nitrogen sources, respectively. Subsequently, starch, NaCl, and MgSO4·7H2O were recognized as the most significant media components with confidence level of above 95% using the Plackett-Burman design. The levels of the three media components were further optimized using RSM employed with Box-Behnken design. Accordingly, a second-order polynomial regression model was fitted into the experimental data. By analyzing the response surface plots as well as using numerical optimization method, the optimal levels for starch, NaCl, and MgSO4·7H2O were determined as 15.6 g/L, 0.8 g/L, and 1.98 g/L, respectively. With the optimized medium, 15.5% increase was observed in antibiotic activity of JAJ18. Results further support the use of RSM for media optimization. To the best of our knowledge, this is the first report of statistical media optimization for antibiotic production in rare actinomyceteNonomuraeaspecies, which will be useful for the development ofNonomuraeacultivation process for efficient antibiotic production on a large scale.

Production and Rheological Properties of Welan Gum Produced by Sphingomonas sp. ATCC 31555 with Different Nitrogen Sources

2017 ◽  
Vol 27 (1) ◽  
pp. 55-63 ◽  
Author(s):  
Xiaopeng Xu ◽  
Zuoming Nie ◽  
Zhiyong Zheng ◽  
Li Zhu ◽  
Xiaobei Zhan
Keyword(s):  
Molecular Weight ◽  
Rheological Properties ◽  
Biomass Concentration ◽  
Bacterial Biomass ◽  
Nitrogen Sources ◽  
Monosaccharide Composition ◽  
Solution Concentration ◽  
Molar Ratio ◽  
Welan Gum ◽  
Beef Extract

This study aimed to investigate the effect of nitrogen sources on the production and rheological properties of welan gum produced by <i>Sphingomonas</i> sp. ATCC 31555. Six different nitrogen sources were used for ATCC 31555 fermentation, and 2 of these were further analyzed due to their more positive influence on welan gum production and bacterial biomass. Bacterial biomass, welan gum yield, welan viscosity, molecular weight, monosaccharide composition, acyl content, and welan structure were analyzed. Welan gum production and the biomass concentration of ATCC 31555 were higher in media containing NaNO<sub>3</sub> and beef extract. Welan viscosity decreased at higher temperatures of 30-90°C, and it increased with a higher welan concentration. In the media containing NaNO<sub>3</sub> (3 g·L<sup>-1</sup>), welan viscosity was higher at 30-70°C and a welan solution concentration of 6-10 g·L<sup>-1</sup>. With a reduced NaNO<sub>3</sub> concentration, the molecular weight of welan gum and the molar ratio of mannose decreased, but the molar ratio of glucuronic acid increased. With different nitrogen sources, the acetyl content of welan gum differed but its structure was similar. NaNO<sub>3</sub> and beef extract facilitated welan production. A reduced NaNO<sub>3</sub> concentration promoted welan viscosity.

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