scholarly journals Genetic diversity of the intimin gene (eae) in non-O157 Shiga toxin-producing Escherichia coli strains in China

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Xi Yang ◽  
Hui Sun ◽  
Ruyue Fan ◽  
Shanshan Fu ◽  
Ji Zhang ◽  
...  
Keyword(s):  
Public Health ◽  
Escherichia Coli ◽  
Genetic Diversity ◽  
Shiga Toxin ◽  
Great Risk ◽  
Critical Role ◽  
Foodborne Pathogen ◽  
Different Sources

AbstractShiga toxin-producing Escherichia coli (STEC) is an important foodborne pathogen. The increasing incidence of non-O157 STEC has posed a great risk to public health. Besides the Shiga toxin (Stx), the adherence factor, intimin, coded by eae gene plays a critical role in STEC pathogenesis. In this study, we investigated the prevalence and polymorphisms of eae gene in non-O157 STEC strains isolated from different sources in China. Among 735 non-O157 STEC strains, eae was present in 70 (9.5%) strains. Eighteen different eae genotypes were identified in 62 eae-positive STEC strains with the nucleotide identities ranging from 86.01% to 99.97%. Among which, seven genotypes were newly identified in this study. The eighteen eae genotypes can be categorized into five eae subtypes, namely β1, γ1, ε1, ζ3 and θ. Associations between eae subtypes/genotypes and serotypes as well as origins of strains were observed in this study. Strains belonging to serotypes O26:H11, O103:H2, O111:H8 are associated with particular eae subtypes, i.e., β1, ε1, θ, respectively. Most strains from diarrheal patients (7/9, 77.8%) carried eae-β1 subtype, while most isolates from cattle (23/26, 88.5%) carried eae-ζ3 subtype. This study demonstrated a genetic diversity of eae gene in non-O157 STEC strains from different sources in China.

scholarly journals Draft Genome Sequences of 12 Shiga Toxin-Producing Escherichia coli Strains Isolated from Dairy Cattle in Portugal

2020 ◽  
Vol 9 (38) ◽  
Author(s):  
Gregor Fiedler ◽  
Andressa Ballem ◽  
Erik Brinks ◽  
Carina Almeida ◽  
Charles M. A. P. Franz ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Genetic Diversity ◽  
Dairy Cattle ◽  
Shiga Toxin ◽  
Draft Genome ◽  
Foodborne Pathogen ◽  
Genome Sequences ◽  
Content Type ◽  
The North ◽  
Contaminated Food

ABSTRACT Shiga toxin-producing Escherichia coli (STEC) is a foodborne pathogen transmitted from animals to humans through contaminated food. Cattle are the main reservoir of STEC, but their genetic diversity is still poorly characterized, especially regarding strains isolated in Portugal. We therefore present the draft genomic sequences of 12 STEC strains isolated from cattle in the north of Portugal.

Mexican unpasteurised fresh cheeses are contaminated with Salmonella spp., non-O157 Shiga toxin producing Escherichia coli and potential uropathogenic E. coli strains: A public health risk

2016 ◽  
Vol 237 ◽  
pp. 10-16 ◽  
Author(s):  
Rosa Guzman-Hernandez ◽  
Araceli Contreras-Rodriguez ◽  
Rosa Hernandez-Velez ◽  
Iza Perez-Martinez ◽  
Ahide Lopez-Merino ◽  
...  
Keyword(s):  
Public Health ◽  
Escherichia Coli ◽  
Health Risk ◽  
Shiga Toxin ◽  
Salmonella Spp ◽  
Public Health Risk ◽  
E Coli

scholarly journals Genetic diversity of the enterohaemolysin gene (ehxA) in non-O157 Shiga toxin-producing Escherichia coli strains in China

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Shanshan Fu ◽  
Xiangning Bai ◽  
Ruyue Fan ◽  
Hui Sun ◽  
Yanmei Xu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Genetic Diversity ◽  
Shiga Toxin

scholarly journals Whole genome sequencing to characterize shiga toxin-producing Escherichia coli O26 in a public health setting

2019 ◽  
Vol 12 (6) ◽  
pp. 884-889 ◽  
Author(s):  
Baha Abdalhamid ◽  
Emily L. Mccutchen ◽  
Alyssa C. Bouska ◽  
Zhang Weiwei ◽  
Brianna Loeck ◽  
...  
Keyword(s):  
Public Health ◽  
Escherichia Coli ◽  
Whole Genome Sequencing ◽  
Genome Sequencing ◽  
Shiga Toxin ◽  
Whole Genome ◽  
Public Health Setting

scholarly journals High-Quality Whole-Genome Sequences for 77 Shiga Toxin-Producing Escherichia coli Strains Generated with PacBio Sequencing

2018 ◽  
Vol 6 (19) ◽  
Author(s):  
Pooja N. Patel ◽  
Rebecca L. Lindsey ◽  
Lisley Garcia-Toledo ◽  
Lori A. Rowe ◽  
Dhwani Batra ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Shiga Toxin ◽  
Foodborne Pathogen ◽  
Severe Illness ◽  
Whole Genome ◽  
Genome Sequences ◽  
Pacbio Sequencing ◽  
High Quality ◽  
Content Type ◽  
Sequencing Platform

ABSTRACT Shiga toxin-producing Escherichia coli (STEC) is an enteric foodborne pathogen that can cause mild to severe illness. Here, we report the availability of high-quality whole-genome sequences for 77 STEC strains generated using the PacBio sequencing platform.

Public Health Microbiology of Shiga Toxin-Producing Escherichia coli

2014 ◽  
Vol 2 (6) ◽  
Author(s):  
Alfredo Caprioli ◽  
Gaia Scavia ◽  
Stefano Morabito
Keyword(s):  
Public Health ◽  
Escherichia Coli ◽  
Shiga Toxin

scholarly journals Molecular Analysis as an Aid To Assess the Public Health Risk of Non-O157 Shiga Toxin-Producing Escherichia coli Strains

2008 ◽  
Vol 74 (7) ◽  
pp. 2153-2160 ◽  
Author(s):  
Brian K. Coombes ◽  
Mark E. Wickham ◽  
Mariola Mascarenhas ◽  
Samantha Gruenheid ◽  
B. Brett Finlay ◽  
...  
Keyword(s):  
Public Health ◽  
Escherichia Coli ◽  
Shiga Toxin ◽  
Genomic Islands ◽  
Epidemic Disease ◽  
The Public ◽  
Virulence Potential ◽  
Uremic Syndrome ◽  
Human Illness ◽  
Globally Distributed

ABSTRACT Shiga toxin-producing Escherichia coli (STEC) strains are commensal bacteria in cattle with high potential for environmental and zoonotic transmission to humans. Although O157:H7 is the most common STEC serotype, there is growing concern over the emergence of more than 200 highly virulent non-O157 STEC serotypes that are globally distributed, several of which are associated with outbreaks and/or severe human illness such as hemolytic-uremic syndrome (HUS) and hemorrhagic colitis. At present, the underlying genetic basis of virulence potential in non-O157 STEC is unknown, although horizontal gene transfer and the acquisition of new pathogenicity islands are an expected origin. We used seropathotype classification as a framework to identify genetic elements that distinguish non-O157 STEC strains posing a serious risk to humans from STEC strains that are not associated with severe and epidemic disease. We report the identification of three genomic islands encoding non-LEE effector (nle) genes and 14 individual nle genes in non-O157 STEC strains that correlate independently with outbreak and HUS potential in humans. The implications for transmissible zoonotic spread and public health are discussed. These results and methods offer a molecular risk assessment strategy to rapidly recognize and respond to non-O157 STEC strains from environmental and animal sources that might pose serious public health risks to humans.

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