scholarly journals Metabolomics for early detection of stress in freshwater alga Poterioochromonas malhamensis exposed to silver nanoparticles

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Wei Liu ◽  
Sanghamitra Majumdar ◽  
Weiwei Li ◽  
Arturo A. Keller ◽  
Vera I. Slaveykova
Keyword(s):  
Silver Nanoparticles ◽  
Early Detection ◽  
Tca Cycle ◽  
Environmental Implications ◽  
Major Toxicity ◽  
Food Vacuoles ◽  
Global Biogeochemical Cycles ◽  
Detection Of Stress ◽  
Poterioochromonas Malhamensis ◽  
And Oxidative Stress

AbstractSilver nanoparticles (AgNPs) are one of the most used engineered nanomaterials. Despite progress in assessing their environmental implications, knowledge gaps exist concerning the metabolic perturbations induced by AgNPs on phytoplankton, essential organisms in global biogeochemical cycles and food-web dynamics. We combine targeted metabolomics, biouptake and physiological response studies to elucidate metabolic perturbations in alga Poterioochromonas malhamensis induced by AgNPs and dissolved Ag. We show time-dependent perturbation of the metabolism of amino acids, nucleotides, fatty acids, tricarboxylic acids, photosynthesis and photorespiration by both Ag-treatments. The results suggest that dissolved Ag ions released by AgNPs are the major toxicity driver; however, AgNPs internalized in food vacuoles contributed to the perturbation of amino acid metabolism, TCA cycle and oxidative stress. The metabolic perturbations corroborate the observed physiological responses. We highlight the potential of metabolomics as a tool for understanding the molecular basis for these metabolic and physiological changes, and for early detection of stress.

scholarly journals Chenopodium murale essential oil alleviates the genotoxicity and oxidative stress of silver nanoparticles in the rat kidney

2019 ◽  
Vol 0 (0) ◽  
pp. 0-0
Author(s):  
Mossad Abdel-Wahhab ◽  
Helmy Ahmed ◽  
Aziza El-Nekeety ◽  
Sekena Abdel-Aziem ◽  
Hafiza Shara ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Silver Nanoparticles ◽  
Essential Oil ◽  
Rat Kidney ◽  
Chenopodium Murale ◽  
And Oxidative Stress

scholarly journals Hepatic Mitochondrial Oxidative Metabolism and Lipogenesis Synergistically Adapt to Mediate Healthy Embryonic-to-Neonatal Transition in Chicken

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Chaitra Surugihalli ◽  
Tom E. Porter ◽  
Angela Chan ◽  
Linda S. Farley ◽  
Meghan Maguire ◽  
...  
Keyword(s):  
Lipid Oxidation ◽  
Metabolic Networks ◽  
Tca Cycle ◽  
Neonatal Development ◽  
Alcoholic Fatty Liver ◽  
Species Production ◽  
Mitochondrial Oxidative Metabolism ◽  
And Oxidative Stress ◽  
Alcoholic Fatty Liver Disease ◽  
Neonatal Transition

AbstractDuring the normal embryonic-to-neonatal development, the chicken liver is subjected to intense lipid burden from high rates of yolk-lipid oxidation and also from the accumulation of the yolk-derived and newly synthesized lipids from carbohydrates. High rates of hepatic lipid oxidation and lipogenesis are also central features of non-alcoholic fatty liver disease (NAFLD) in both rodents and humans, but is associated with impaired insulin signaling, dysfunctional mitochondrial energetics and oxidative stress. However, these adverse effects are not apparent in the liver of embryonic and neonatal chicken, despite lipid burden. Utilizing comprehensive metabolic profiling, we identify that steady induction of hepatic mitochondrial tricarboxylic acid (TCA) cycle and lipogenesis are central features of embryonic-to-neonatal transition. More importantly, the induction of TCA cycle and lipogenesis occurred together with the downregulation of hepatic β-oxidation and ketogenesis in the neonatal chicken. This synergistic remodeling of hepatic metabolic networks blunted inflammatory onset, prevented accumulation of lipotoxic intermediates (ceramides and diacylglycerols) and reduced reactive oxygen species production during embryonic-to-neonatal development. This dynamic remodeling of hepatic mitochondrial oxidative flux and lipogenesis aids in the healthy embryonic-to-neonatal transition in chicken. This natural physiological system could help identify mechanisms regulating mitochondrial function and lipogenesis, with potential implications towards treatment of NAFLD.

scholarly journals Silver nanoparticles cause osmoregulatory impairment and oxidative stress in Caspian kutum (Rutilus kutum, Kamensky 1901)

2017 ◽  
Vol 189 (9) ◽  
Author(s):  
Fatemeh F. Masouleh ◽  
Bagher M. Amiri ◽  
Alireza Mirvaghefi ◽  
Hossein Ghafoori ◽  
Steffen S. Madsen
Keyword(s):  
Oxidative Stress ◽  
Silver Nanoparticles ◽  
And Oxidative Stress

scholarly journals Low iron-induced small RNA BrrF regulates central metabolism and oxidative stress responses in Burkholderia cenocepacia

2019 ◽  
Author(s):  
Andrea Sass ◽  
Tom Coenye
Keyword(s):  
Oxidative Stress ◽  
Small Rna ◽  
Deletion Mutant ◽  
Wild Type Strain ◽  
Tca Cycle ◽  
Burkholderia Cenocepacia ◽  
Iron Starvation ◽  
Iron Depletion ◽  
In The Wild ◽  
And Oxidative Stress

AbstractBrrF is a Fur-regulated small RNA highly upregulated in Burkholderia cenocepacia under conditions of iron depletion. Its computationally predicted targets include iron-containing enzymes of the tricarboxylic acid (TCA) cycle such as aconitase and succinate dehydrogenase, as well as iron-containing enzymes responsible for the oxidative stress response, such as superoxide dismutase and catalase. Phenotypic and gene expression analysis of BrrF deletion and overexpression mutants show that the regulation of these genes is BrrF-dependent. Expression of acnA, fumA, sdhA and sdhC was downregulated during iron depletion in the wild type strain, but not in a BrrF deletion mutant. TCA cycle genes not predicted as target for BrrF were not affected in the same manner by iron depletion. Likewise, expression of sodB and katB was dowregulated during iron depletion in the wild type strain, but not in a BrrF deletion mutant. BrrF overexpression reduced aconitase and superoxide dismutase activities and increased sensitivity to hydrogen peroxide. All phenotypes and gene expression changes of the BrrF deletion mutant could be complemented by overexpressing BrrF in trans. Overall, BrrF acts as a regulator of central metabolism and oxidative stress response, possibly as an iron-sparing measure to maintain iron homeostasis under conditions of iron starvation.ImportanceRegulatory small RNAs play an essential role in maintaining cell homeostasis in bacteria in response to environmental stresses such as iron starvation. Prokaryotes generally encode a large number of RNA regulators, yet their identification and characterisation is still in its infancy for most bacterial species. Burkholderia cenocepacia is an opportunistic pathogen with high innate antimicrobial resistance, which can cause the often fatal cepacia syndrome in individuals with cystic fibrosis. In this study we characterise a small RNA which is involved in the response to iron starvation, a condition that pathogenic bacteria are likely to encounter in the host.

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