Electrocoalescence of paired droplets encapsulated in double-emulsion drops

Lab on a Chip ◽  
2016 ◽  
Vol 16 (22) ◽  
pp. 4313-4318 ◽  
Author(s):  
Yankan Jia ◽  
Yukun Ren ◽  
Weiyu Liu ◽  
Likai Hou ◽  
Ye Tao ◽  
...  

We utilize an ac electric field to trigger the on-demand fusion of two aqueous cores inside water-in-oil-in-water (W/O/W) double-emulsion drops.

Soft Matter ◽  
2019 ◽  
Vol 15 (6) ◽  
pp. 1388-1395 ◽  
Author(s):  
Julie Perrotton ◽  
Rubén Ahijado-Guzmán ◽  
Lara H. Moleiro ◽  
Berta Tinao ◽  
Andrés Guerrero-Martinez ◽  
...  

Water-in-oil-in-water double emulsion drops, fabricated using capillary microfluidics, enable the formation of vesicles with hybrid lipid/nanoparticle membranes.


2017 ◽  
Vol 9 (17) ◽  
pp. 2511-2516 ◽  
Author(s):  
Likai Hou ◽  
Yukun Ren ◽  
Yankai Jia ◽  
Xiaokang Deng ◽  
Zheng Tang ◽  
...  

This work reports a simple microfluidic method for one-step encapsulation of two reagents with varying concentrations in water-in-oil-in-water (W/O/W) double-emulsion drops.


Lab on a Chip ◽  
2018 ◽  
Vol 18 (7) ◽  
pp. 1121-1129 ◽  
Author(s):  
Yankai Jia ◽  
Yukun Ren ◽  
Likai Hou ◽  
Weiyu Liu ◽  
Tianyi Jiang ◽  
...  

We utilize electric fields to trigger the on-demand release of different cargos that are encapsulated in water-in-oil-in-water (W/O/W) double-emulsion droplets.


Micromachines ◽  
2020 ◽  
Vol 11 (4) ◽  
pp. 444 ◽  
Author(s):  
Jianhua Guo ◽  
Lihua Hou ◽  
Junpeng Hou ◽  
Jiali Yu ◽  
Qingming Hu

Microcapsules are attractive core-shell configurations for studies of controlled release, biomolecular sensing, artificial microbial environments, and spherical film buckling. However, the production of microcapsules with ultra-thin shells remains a challenge. Here we develop a simple and practical osmolarity-controlled swelling method for the mass production of monodisperse microcapsules with ultra-thin shells via water-in-oil-in-water (W/O/W) double-emulsion drops templating. The size and shell thickness of the double-emulsion drops are precisely tuned by changing the osmotic pressure between the inner cores and the suspending medium, indicating the practicability and effectiveness of this swelling method in tuning the shell thickness of double-emulsion drops and the resultant microcapsules. This method enables the production of microcapsules even with an ultra-thin shell less than hundreds of nanometers, which overcomes the difficulty in producing ultra-thin-shell microcapsules using the classic microfluidic emulsion technologies. In addition, the ultra-thin-shell microcapsules can maintain their intact spherical shape for up to 1 year without rupturing in our long-term observation. We believe that the osmolarity-controlled swelling method will be useful in generating ultra-thin-shell polydimethylsiloxane (PDMS) microcapsules for long-term encapsulation, and for thin film folding, buckling and rupturing investigation.


2019 ◽  
Vol 92 (1) ◽  
pp. 1147-1153 ◽  
Author(s):  
Adrian J. T. Teo ◽  
Say Hwa Tan ◽  
Nam-Trung Nguyen

2015 ◽  
Vol 108 ◽  
pp. 183-191 ◽  
Author(s):  
Reza Riahifar ◽  
Babak Raissi ◽  
Cyrus Zamani ◽  
Ehsan Marzbanrad

2014 ◽  
Vol 99 ◽  
pp. 160-163 ◽  
Author(s):  
Hiroshi Kimura ◽  
Mao Ueno ◽  
Shinya Takahashi ◽  
Akira Tsuchida ◽  
Keiichi Kurosaka

Author(s):  
Madhusmita Mishra ◽  
Anil Krishna Koduri ◽  
Aman Chandra ◽  
D. Roy Mahapatra ◽  
G. M. Hegde

This paper reports on the characterization of an integrated micro-fluidic platform for controlled electrical lysis of biological cells and subsequent extraction of intracellular biomolecules. The proposed methodology is capable of high throughput electrical cell lysis facilitated by nano-composite coated electrodes. The nano-composites are synthesized using Carbon Nanotube and ZnO nanorod dispersion in polymer. Bacterial cells are used to demonstrate the lysis performance of these nanocomposite electrodes. Investigation of electrical lysis in the microchannel is carried out under different parameters, one with continuous DC application and the other under DC biased AC electric field. Lysis in DC field is dependent on optimal field strength and governed by the cell type. By introducing the AC electrical field, the electrokinetics is controlled to prevent cell clogging in the micro-channel and ensure uniform cell dispersion and lysis. Lysis mechanism is analyzed with time-resolved fluorescence imaging which reveal the time scale of electrical lysis and explain the dynamic behavior of GFP-expressing E. coli cells under the electric field induced by nanocomposite electrodes. The DNA and protein samples extracted after lysis are compared with those obtained from a conventional chemical lysis method by using a UV–Visible spectroscopy and fluorimetry. The paper also focuses on the mechanistic understanding of the nano-composite coating material and the film thickness on the leakage charge densities which lead to differential lysis efficiency.


Sign in / Sign up

Export Citation Format

Share Document