Label-free and sensitive detection of uracil-DNA glycosylase using exponential real-time rolling circle amplification

2018 ◽  
Vol 10 (20) ◽  
pp. 2405-2410 ◽  
Author(s):  
Yuan Xu ◽  
Yun-Xi Cui ◽  
Qiu-Ge Zhao ◽  
An-Na Tang ◽  
De-Ming Kong

Schematic representation of an exponential rolling circle amplification-based uracil-DNA glycosylase activity-sensing platform.

ACS Omega ◽  
2019 ◽  
Vol 4 (2) ◽  
pp. 3881-3886 ◽  
Author(s):  
Lijuan Dong ◽  
Xuange Zhang ◽  
Yaru Li ◽  
Fangjie E ◽  
Jiangyan Zhang ◽  
...  

2015 ◽  
Vol 51 (5) ◽  
pp. 929-932 ◽  
Author(s):  
Jing Tao ◽  
Panshu Song ◽  
Yusuke Sato ◽  
Seiichi Nishizawa ◽  
Norio Teramae ◽  
...  

A label-free fluorescent method has been developed for sensitive detection of uracil-DNA glycosylase activity as well as UDG inhibitors.


2021 ◽  
Vol 57 (16) ◽  
pp. 2041-2044
Author(s):  
Su Jiang ◽  
Meng Liu ◽  
Wei Tantai ◽  
Qinfeng Xu ◽  
Xiaoran Zou ◽  
...  

An aptamer-mediated histone modification site-specific rolling circle amplification is developed for label-free detection of histone-modifying enzyme at the femtomolar level.


2022 ◽  
Author(s):  
Xiaoxia Han ◽  
Yue-Ying Li ◽  
Zi-Yue Wang ◽  
Ling-Zhi Liu ◽  
Bing-Jie Liu ◽  
...  

We develop for the first time a label-free fluorescent method for sensitive detection of fat mass and obesity-associated protein (FTO) activity using MazF-mediated primer generation rolling circle amplification. This method...


The Analyst ◽  
2018 ◽  
Vol 143 (7) ◽  
pp. 1593-1598 ◽  
Author(s):  
Juan Song ◽  
Fei Yin ◽  
Xia Li ◽  
Na Dong ◽  
Yingjie Zhu ◽  
...  

We developed a novel approach to sensitive detection of formamidopyrimidine-DNA glycosylase activity based on target-induced selfprimed rolling circle amplification and magnetic nanoprobes.


2018 ◽  
Vol 54 (51) ◽  
pp. 6991-6994 ◽  
Author(s):  
Yan Zhang ◽  
Qing-nan Li ◽  
Chen-chen Li ◽  
Chun-yang Zhang

We develop a label-free and high-throughput bioluminescence method for the sensitive detection of uracil DNA glycosylase through tricyclic cascade signal amplification.


Biosensors ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 222
Author(s):  
Chenxin Fang ◽  
Ping Ouyang ◽  
Yuxing Yang ◽  
Yang Qing ◽  
Jialun Han ◽  
...  

A microRNA (miRNA) detection platform composed of a rolling circle amplification (RCA) system and an allosteric deoxyribozyme system is proposed, which can detect miRNA-21 rapidly and efficiently. Padlock probe hybridization with the target miRNA is achieved through complementary base pairing and the padlock probe forms a closed circular template under the action of ligase; this circular template results in RCA. In the presence of DNA polymerase, RCA proceeds and a long chain with numerous repeating units is formed. In the presence of single-stranded DNA (H1 and H2), multi-component nucleic acid enzymes (MNAzymes) are formed that have the ability to cleave substrates. Finally, substrates containing fluorescent and quenching groups and magnesium ions are added to the system to activate the MNAzyme and the substrate cleavage reaction, thus achieving fluorescence intensity amplification. The RCA–MNAzyme system has dual signal amplification and presents a sensing platform that demonstrates broad prospects in the analysis and detection of nucleic acids.


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