Label-free and sensitive detection of uracil-DNA glycosylase using exponential real-time rolling circle amplification

2018 ◽  
Vol 10 (20) ◽  
pp. 2405-2410 ◽  
Author(s):  
Yuan Xu ◽  
Yun-Xi Cui ◽  
Qiu-Ge Zhao ◽  
An-Na Tang ◽  
De-Ming Kong
Keyword(s):  
Real Time ◽  
Rolling Circle Amplification ◽  
Sensitive Detection ◽  
Dna Glycosylase ◽  
Label Free ◽  
Uracil Dna Glycosylase ◽  
Schematic Representation ◽  
Rolling Circle ◽  
Sensing Platform ◽  
Activity Sensing

Schematic representation of an exponential rolling circle amplification-based uracil-DNA glycosylase activity-sensing platform.

A label-free and sensitive fluorescent method for the detection of uracil-DNA glycosylase activity

2015 ◽  
Vol 51 (5) ◽  
pp. 929-932 ◽  
Author(s):  
Jing Tao ◽  
Panshu Song ◽  
Yusuke Sato ◽  
Seiichi Nishizawa ◽  
Norio Teramae ◽  
...  
Keyword(s):  
Sensitive Detection ◽  
Dna Glycosylase ◽  
Label Free ◽  
Uracil Dna Glycosylase ◽  
Fluorescent Method

A label-free fluorescent method has been developed for sensitive detection of uracil-DNA glycosylase activity as well as UDG inhibitors.

Aptamer-mediated rolling circle amplification for label-free and sensitive detection of histone acetyltransferase activity

2021 ◽  
Vol 57 (16) ◽  
pp. 2041-2044
Author(s):  
Su Jiang ◽  
Meng Liu ◽  
Wei Tantai ◽  
Qinfeng Xu ◽  
Xiaoran Zou ◽  
...  
Keyword(s):  
Histone Modification ◽  
Histone Acetyltransferase ◽  
Rolling Circle Amplification ◽  
Sensitive Detection ◽  
Label Free ◽  
Site Specific ◽  
Rolling Circle ◽  
Acetyltransferase Activity ◽  
Label Free Detection ◽  
Histone Acetyltransferase Activity

An aptamer-mediated histone modification site-specific rolling circle amplification is developed for label-free detection of histone-modifying enzyme at the femtomolar level.

Label-free and sensitive detection of RNA demethylase FTO with primer generation rolling circle amplification

2022 ◽  
Author(s):  
Xiaoxia Han ◽  
Yue-Ying Li ◽  
Zi-Yue Wang ◽  
Ling-Zhi Liu ◽  
Bing-Jie Liu ◽  
...  
Keyword(s):  
Fat Mass ◽  
Rolling Circle Amplification ◽  
Sensitive Detection ◽  
Label Free ◽  
Rolling Circle ◽  
Fluorescent Method ◽  
First Time

We develop for the first time a label-free fluorescent method for sensitive detection of fat mass and obesity-associated protein (FTO) activity using MazF-mediated primer generation rolling circle amplification. This method...

Sensitive detection of formamidopyrimidine-DNA glycosylase activity based on target-induced self-primed rolling circle amplification and magnetic nanoprobes

The Analyst ◽  
2018 ◽  
Vol 143 (7) ◽  
pp. 1593-1598 ◽  
Author(s):  
Juan Song ◽  
Fei Yin ◽  
Xia Li ◽  
Na Dong ◽  
Yingjie Zhu ◽  
...  
Keyword(s):  
Rolling Circle Amplification ◽  
Sensitive Detection ◽  
Dna Glycosylase ◽  
Rolling Circle ◽  
Novel Approach

We developed a novel approach to sensitive detection of formamidopyrimidine-DNA glycosylase activity based on target-induced selfprimed rolling circle amplification and magnetic nanoprobes.

Label-free and high-throughput bioluminescence detection of uracil-DNA glycosylase in cancer cells through tricyclic cascade signal amplification

2018 ◽  
Vol 54 (51) ◽  
pp. 6991-6994 ◽  
Author(s):  
Yan Zhang ◽  
Qing-nan Li ◽  
Chen-chen Li ◽  
Chun-yang Zhang
Keyword(s):  
Cancer Cells ◽  
High Throughput ◽  
Signal Amplification ◽  
Sensitive Detection ◽  
Dna Glycosylase ◽  
Label Free ◽  
Uracil Dna Glycosylase ◽  
Bioluminescence Method

We develop a label-free and high-throughput bioluminescence method for the sensitive detection of uracil DNA glycosylase through tricyclic cascade signal amplification.

scholarly journals MiRNA Detection Using a Rolling Circle Amplification and RNA-Cutting Allosteric Deoxyribozyme Dual Signal Amplification Strategy

Biosensors ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 222
Author(s):  
Chenxin Fang ◽  
Ping Ouyang ◽  
Yuxing Yang ◽  
Yang Qing ◽  
Jialun Han ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Rolling Circle Amplification ◽  
Padlock Probe ◽  
Rolling Circle ◽  
Sensing Platform ◽  
Mirna Detection ◽  
Substrate Cleavage ◽  
Amplification Strategy ◽  
Circular Template ◽  
Dual Signal Amplification

A microRNA (miRNA) detection platform composed of a rolling circle amplification (RCA) system and an allosteric deoxyribozyme system is proposed, which can detect miRNA-21 rapidly and efficiently. Padlock probe hybridization with the target miRNA is achieved through complementary base pairing and the padlock probe forms a closed circular template under the action of ligase; this circular template results in RCA. In the presence of DNA polymerase, RCA proceeds and a long chain with numerous repeating units is formed. In the presence of single-stranded DNA (H1 and H2), multi-component nucleic acid enzymes (MNAzymes) are formed that have the ability to cleave substrates. Finally, substrates containing fluorescent and quenching groups and magnesium ions are added to the system to activate the MNAzyme and the substrate cleavage reaction, thus achieving fluorescence intensity amplification. The RCA–MNAzyme system has dual signal amplification and presents a sensing platform that demonstrates broad prospects in the analysis and detection of nucleic acids.

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