Aptamer-mediated rolling circle amplification for label-free and sensitive detection of histone acetyltransferase activity

2021 ◽  
Vol 57 (16) ◽  
pp. 2041-2044
Author(s):  
Su Jiang ◽  
Meng Liu ◽  
Wei Tantai ◽  
Qinfeng Xu ◽  
Xiaoran Zou ◽  
...  
Keyword(s):  
Histone Modification ◽  
Histone Acetyltransferase ◽  
Rolling Circle Amplification ◽  
Sensitive Detection ◽  
Label Free ◽  
Site Specific ◽  
Rolling Circle ◽  
Acetyltransferase Activity ◽  
Label Free Detection ◽  
Histone Acetyltransferase Activity

An aptamer-mediated histone modification site-specific rolling circle amplification is developed for label-free detection of histone-modifying enzyme at the femtomolar level.

Unique electrocatalytic activity of a nucleic acid-mimicking coordination polymer for the sensitive detection of coenzyme A and histone acetyltransferase activity

2015 ◽  
Vol 51 (99) ◽  
pp. 17611-17614 ◽  
Author(s):  
Yufang Hu ◽  
Siyu Chen ◽  
Yitao Han ◽  
Hongjun Chen ◽  
Qin Wang ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Coordination Polymer ◽  
Electrocatalytic Activity ◽  
Histone Acetyltransferase ◽  
Coenzyme A ◽  
Sensitive Detection ◽  
Acetyltransferase Activity ◽  
Histone Acetyltransferase Activity

Unique electrocatalytic activity of a nucleic acid-mimicking CoA–Ag(i) CP was discovered and used for the sensitive detection of CoA and HAT activity.

Carbon nanotube enhanced label-free detection of microRNAs based on hairpin probe triggered solid-phase rolling-circle amplification

Nanoscale ◽  
2015 ◽  
Vol 7 (3) ◽  
pp. 987-993 ◽  
Author(s):  
Qianqian Tian ◽  
Ying Wang ◽  
Ruijie Deng ◽  
Lei Lin ◽  
Yang Liu ◽  
...  
Keyword(s):  
Carbon Nanotube ◽  
Solid Phase ◽  
Rolling Circle Amplification ◽  
Label Free ◽  
Rolling Circle ◽  
Hairpin Probe ◽  
Label Free Detection

Label-free and sensitive detection of RNA demethylase FTO with primer generation rolling circle amplification

2022 ◽  
Author(s):  
Xiaoxia Han ◽  
Yue-Ying Li ◽  
Zi-Yue Wang ◽  
Ling-Zhi Liu ◽  
Bing-Jie Liu ◽  
...  
Keyword(s):  
Fat Mass ◽  
Rolling Circle Amplification ◽  
Sensitive Detection ◽  
Label Free ◽  
Rolling Circle ◽  
Fluorescent Method ◽  
First Time

We develop for the first time a label-free fluorescent method for sensitive detection of fat mass and obesity-associated protein (FTO) activity using MazF-mediated primer generation rolling circle amplification. This method...

Label-free and sensitive detection of uracil-DNA glycosylase using exponential real-time rolling circle amplification

2018 ◽  
Vol 10 (20) ◽  
pp. 2405-2410 ◽  
Author(s):  
Yuan Xu ◽  
Yun-Xi Cui ◽  
Qiu-Ge Zhao ◽  
An-Na Tang ◽  
De-Ming Kong
Keyword(s):  
Real Time ◽  
Rolling Circle Amplification ◽  
Sensitive Detection ◽  
Dna Glycosylase ◽  
Label Free ◽  
Uracil Dna Glycosylase ◽  
Schematic Representation ◽  
Rolling Circle ◽  
Sensing Platform ◽  
Activity Sensing

Schematic representation of an exponential rolling circle amplification-based uracil-DNA glycosylase activity-sensing platform.

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