Ultra-rapid modulation of neurite outgrowth in a gigahertz acoustic streaming system

Lab on a Chip ◽  
2021 ◽  
Author(s):  
Shan He ◽  
Zefang Wang ◽  
Wei Pang ◽  
Chang Liu ◽  
Miaosen Zhang ◽  
...  
Keyword(s):  
Single Cell ◽  
Neurite Outgrowth ◽  
Pc12 Cell ◽  
Cell Structure ◽  
Acoustic Streaming ◽  
Structure Change ◽  
System P ◽  
Rapid Modulation

A gigahertz acoustic streaming effect stimulates PC12 cell structure change within 10 min, and produces a higher ratio of neurite-bearing cells compared to NGF stimulation. This method can be of single-cell modulation due to microminiaturization.

Characterization of a PC12 cell sub-clone (PC12-C41) with enhanced neurite outgrowth capacity: implications for a modulatory role of high molecular weight tau in neuritogenesis

1993 ◽  
Vol 106 (2) ◽  
pp. 611-626 ◽  
Author(s):  
K.K. Teng ◽  
I.S. Georgieff ◽  
J.M. Aletta ◽  
J. Nunez ◽  
M.L. Shelanski ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Neurite Outgrowth ◽  
Pc12 Cell ◽  
Cell Types ◽  
Neuronal Morphology ◽  
Cytoskeletal Proteins ◽  
Parental Line ◽  
Pheochromocytoma Cells ◽  
Tau Expression

To address the means by which diversity of neuronal morphology is generated, we have isolated and characterized naturally occurring variants of rat PC12 pheochromocytoma cells that exhibit altered neurite outgrowth properties in response to nerve growth factor (NGF). We describe here a PC12 cell sub-clone, designated PC12-clone 41 (PC12-C41), that displays significant increases in neurite abundance and stability when compared with the parental line. This difference does not appear to be due to an altered sensitivity or responsiveness to NGF or to a more rapid rate of neurite extension. Because of the role of the cytoskeleton in neuritogenesis, we examined a panel of the major cytoskeletal proteins (MAP 1.2/1B, beta-tubulin, chartins, peripherin, and high and low molecular weight (HMW and LMW) taus) whose levels and/or extent of phosphorylation are regulated by NGF in PC12 cultures. Although most cytoskeletal proteins showed little difference between PC12 and PC12-C41 cells (+/- NGF treatment), there was a significant contrast between the two lines with respect to tau expression. In particular, while NGF increases the total specific levels of tau in both cell types to similar extents (by about twofold), the proportion comprising HMW tau is threefold higher in the PC12-C41 clone than in PC12 cells. A comparable difference was observed under substratum conditions that were non-permissive for neurite outgrowth and so this effect was not merely a consequence of the differential neuritogenic capacities of the two lines. The distinction between the expression of HMW and LMW taus in PC12 and PC12-C41 cells (+/- NGF) was also observed at the level of the messages encoding these proteins. Such findings indicate that initiation of neurite outgrowth in PC12 cultures does not require a massive induction of tau expression and raise the possibility that HMW and LMW taus may have differential capacities for modulating neuronal morphology.

Notch Signaling Inhibits PC12 Cell Neurite Outgrowth via RBP-J-Dependent and -Independent Mechanisms

2002 ◽  
Vol 24 (1) ◽  
pp. 79-88 ◽  
Author(s):  
Oren A. Levy ◽  
James J. Lah ◽  
Allan I. Levey
Keyword(s):  
Notch Signaling ◽  
Neurite Outgrowth ◽  
Pc12 Cell

scholarly journals A Newly Designed Modular ZnBr2 Single Cell Structure

Batteries ◽  
2020 ◽  
Vol 6 (2) ◽  
pp. 27
Author(s):  
Zongqiang Pang ◽  
Yutao Gong ◽  
Ming Yuan ◽  
Xin Li
Keyword(s):  
Energy Density ◽  
Single Cell ◽  
Modular Design ◽  
High Reliability ◽  
Low Cost ◽  
Cell Structure ◽  
Performance Testing ◽  
High Energy ◽  
High Energy Density ◽  
Symmetrical Structure

We describe a ZnBr2 single cell which has a highly modular symmetrical structure. With designed polyethylene shell frames, membrane frame and composite titanium-carbon felt electrodes, it has a higher energy density and is more flexible compared with traditional flow batteries. We repeatedly tested its performance, which showed good tightness, high reliability and a high energy efficiency of 75%. Due to the special symmetrical structure and modular design, it is easy to assemble and disassemble, which makes it suitable as a test platform for electrodes, membranes and electrolyte performance testing. The designed modular flow cell has low cost and high energy density, and can provide good guidance for flow battery research.

Peptide-catalyzed, bioinspired silicification for single-cell encapsulation in the imidazole-buffered system

2015 ◽  
Vol 51 (25) ◽  
pp. 5523-5525 ◽  
Author(s):  
Ji Hun Park ◽  
Insung S. Choi ◽  
Sung Ho Yang
Keyword(s):  
Single Cell ◽  
Cell Encapsulation ◽  
Yeast Cells ◽  
Mild Conditions ◽  
System P ◽  
Glass Sponges ◽  
Silica Encapsulation ◽  
Single Cell Encapsulation

Cytoprotective silica encapsulation of individual yeast cells is achieved by a peptide-catalyzed silicification under mild conditions, inspired by glass sponges.

Spectromicroscopic observation of a live single cell in a biocompatible liquid-enclosing graphene system

Nanoscale ◽  
2018 ◽  
Vol 10 (1) ◽  
pp. 150-157 ◽  
Author(s):  
Seong Uk Yu ◽  
Hwiwon Lee ◽  
Woo Jong Cho ◽  
Chulhyun Kim ◽  
Moon Cheol Kang ◽  
...  
Keyword(s):  
Single Cell ◽  
Live Cells ◽  
X Ray ◽  
Biochemical Responses ◽  
System P ◽  
Scanning Transmission

Scanning transmission X-ray microscopy combined with liquid-enclosing graphene system offering biocompatible wet environment allows on-the-spot visualization of biochemical responses of live cells.

Statistical single-cell analysis of cell cycle-dependent quantum dot cytotoxicity and cellular uptake using a microfluidic system

RSC Advances ◽  
2014 ◽  
Vol 4 (47) ◽  
pp. 24929-24934 ◽  
Author(s):  
Jing Wu ◽  
Haifang Li ◽  
Qiushui Chen ◽  
Xuexia Lin ◽  
Wu Liu ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Quantum Dot ◽  
Single Cell ◽  
Cellular Uptake ◽  
Single Cell Analysis ◽  
Single Cells ◽  
Microfluidic System ◽  
Cell Analysis ◽  
System P ◽  
Cycle Dependent

The response of single cells in different cell cycle phases to QD cytotoxicity studied on a microfluidic device.

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