Engineering 3D-printed core-shell hydrogel scaffolds reinforced with hybrid hydroxyapatite/polycaprolactone nanoparticles for in vivo bone regeneration

2021 ◽  
Author(s):  
Salma Essam El-Habashy ◽  
Amal ElKamel ◽  
Marwa Essawy ◽  
Elsayeda-Zeinab Abdelfattah ◽  
Hoda M Eltaher
Keyword(s):  
3D Printing ◽  
Bone Regeneration ◽  
Composite Hydrogel ◽  
Core Shell ◽  
Hydrogel Scaffolds ◽  
3D Printed ◽  
Indispensable Tool

The versatility of 3D printing has rendered it an indispensable tool for the fabrication of composite hydrogel scaffolds, offering bone biomimetic features through inorganic and biopolymeric components as promising platforms...

scholarly journals Vascularized Bone Regeneration Accelerated by 3D-Printed Nanosilicate-Functionalized Polycaprolactone Scaffold

2021 ◽  
Author(s):  
Xiongcheng Xu ◽  
Long Xiao ◽  
Yanmei Xu ◽  
Jin Zhuo ◽  
Xue Yang ◽  
...  
Keyword(s):  
3D Printing ◽  
Bone Regeneration ◽  
Bone Repair ◽  
Cytokine Secretion ◽  
Printing Technology ◽  
3D Printing Technology ◽  
3D Printed ◽  
Vascularized Bone

Abstract Critical oral-maxillofacial bone defects, damaged by trauma and tumors, not only affect the physiological functions and mental health of patients but are also highly challenging to reconstruct. Personalized biomaterials customized by 3D printing technology have the potential to match oral-maxillofacial bone repair and regeneration requirements. Laponite nanosilicates have been added to biomaterials to achieve biofunctional modification owing to their excellent biocompatibility and bioactivity. Herein, porous nanosilicate-functionalized polycaprolactone (PCL/LAP) was fabricated by 3D printing technology, and its bioactivities in bone regeneration were investigated in vitro and in vivo. In vitro experiments demonstrated that PCL/LAP exhibited good cytocompatibility and enhanced the viability of BMSCs. PCL/LAP functioned to stimulate osteogenic differentiation of BMSCs at the mRNA and protein levels and elevated angiogenic gene expression and cytokine secretion. Moreover, BMSCs cultured on PCL/LAP promoted the angiogenesis potential of endothelial cells by angiogenic cytokine secretion. Then, PCL/LAP scaffolds were implanted into the calvarial defect model. Toxicological safety of PCL/LAP was confirmed, and significant enhancement of vascularized bone formation was observed. Taken together, 3D-printed PCL/LAP scaffolds with brilliant osteogenesis to enhance bone regeneration could be envisaged as an outstanding bone substitute for a promising change in oral-maxillofacial bone defect reconstruction.

Melatonin decorated 3D-printed beta-tricalcium phosphate scaffolds promoting bone regeneration in a rat calvarial defect model

2019 ◽  
Vol 7 (20) ◽  
pp. 3250-3259 ◽  
Author(s):  
Yali Miao ◽  
Yunhua Chen ◽  
Xiao Liu ◽  
Jingjing Diao ◽  
Naru Zhao ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Tricalcium Phosphate ◽  
Calvarial Defect ◽  
Defect Model ◽  
Beta Tricalcium Phosphate ◽  
3D Printed ◽  
Rat Calvarial Defect

3D-printed β-TCP scaffolds decorated with melatonin via dopamine mussel-inspired chemistry enhance the osteogenesis and in vivo bone regeneration.

Mechanical Testing and Reliability Analysis for 3D Printed Cubic Lattices

2020 ◽  
Author(s):  
Nitin Nagesh Kulkarni ◽  
Stephen Ekwaro-Osire ◽  
Paul F. Egan
Keyword(s):  
3D Printing ◽  
Average Length ◽  
Input Parameter ◽  
Chi Square ◽  
Reliability Method ◽  
Input Parameters ◽  
Chi Square Test ◽  
Length Width ◽  
3D Printed

Abstract 3D printing has enabled new avenues to design and fabricate diverse structures for engineering applications, such as mechanically efficient lattices. Lattices are useful as implants for biological applications for supporting in vivo loads. However, inconsistencies in 3D printing motivates a need to quantify uncertainties contributing to mechanical failure using probabilistic analysis. Here, 50 cubic unit cell lattice samples were printed and tested with designs of 50% porosity, 500-micron beam diameters, and 3.5mm length, width, and height dimensions. The average length, width, and height measurements ranged from 3.47mm to 3.48mm. The precision in printing with a 95% confidence level was greater than 99.8%. Lattice elastic moduli ranged from about 270 MPa to 345 MPa, with a mean of 305 MPa. Probabilistic analyses were conducted with NESSUS software. The distributions of input parameters were determined using a chi-square test. The first-order reliability method was used to calculate the probability of failure and sensitivity of each input parameter. The elastic modulus was the most sensitive among all input parameters, with 57% of the total sensitivity. The study quantified printing inconsistencies and sensitives using empirical evidence and is a significant step forward for designing 3D printed parts for mechanical applications.

Engineered macroporous hydrogel scaffolds via pickering emulsions stabilized by MgO nanoparticles promote bone regeneration

2020 ◽  
Vol 8 (28) ◽  
pp. 6100-6114
Author(s):  
Haotian Pan ◽  
Huichang Gao ◽  
Qingtao Li ◽  
Zefeng Lin ◽  
Qi Feng ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Composite Hydrogel ◽  
Pickering Emulsions ◽  
Mgo Nanoparticles ◽  
Hydrogel Scaffolds

A novel macroporous composite hydrogel scaffolds were developed by using Pickering emulsions stabilized by MgO NPs for application in the field of bone regeneration.

scholarly journals Local controlled release of simvastatin and PDGF from core/shell microspheres promotes bone regeneration in vivo

RSC Advances ◽  
2017 ◽  
Vol 7 (32) ◽  
pp. 19621-19629 ◽  
Author(s):  
Mingming Yan ◽  
Jiangdong Ni ◽  
Hongwei Shen ◽  
Deye Song ◽  
Muliang Ding ◽  
...  
Keyword(s):  
Controlled Release ◽  
Bone Formation ◽  
Bone Regeneration ◽  
Core Shell ◽  
Potent Stimulator

Simvastatin is demonstrated to be a potent stimulator for bone formation.

3D-printed alginate/phenamil composite scaffolds constituted with microsized core–shell struts for hard tissue regeneration

RSC Advances ◽  
2015 ◽  
Vol 5 (37) ◽  
pp. 29335-29345 ◽  
Author(s):  
KyoungHo Lee ◽  
Cho-Rong Seo ◽  
Jin-Mo Ku ◽  
Hyeongjin Lee ◽  
Hyeon Yoon ◽  
...  
Keyword(s):  
3D Printing ◽  
Tissue Regeneration ◽  
Composite Scaffold ◽  
Core Shell ◽  
Coating Process ◽  
Hard Tissue ◽  
Composite Scaffolds ◽  
Combined Process ◽  
3D Printed

A new composite scaffold consisting of poly(ε-caprolactone), alginate, and phenamil was manufactured by a combined process, 3D-printing and coating process, for hard tissue regeneration.

scholarly journals The Surface Characterisation of Fused Filament Fabricated (FFF) 3D Printed PEEK/Hydroxyapatite Composites

Polymers ◽  
2021 ◽  
Vol 13 (18) ◽  
pp. 3117
Author(s):  
Krzysztof Rodzeń ◽  
Mary Josephine McIvor ◽  
Preetam K. Sharma ◽  
Jonathan G. Acheson ◽  
Alistair McIlhagger ◽  
...  
Keyword(s):  
3D Printing ◽  
Photoelectron Spectroscopy ◽  
High Performance ◽  
Secondary Ion Mass Spectrometry ◽  
Fused Filament Fabrication ◽  
Surface Characterisation ◽  
X Ray ◽  
3D Printed

Polyetheretherketone (PEEK) is a high-performance thermoplastic polymer which has found increasing application in orthopaedics and has shown a lot of promise for ‘made-to-measure’ implants via additive manufacturing approaches. However, PEEK is bioinert and needs to undergo surface modification to make it at least osteoconductive to ensure a more rapid, improved, and stable fixation that will last longer in vivo. One approach to solving this issue is to modify PEEK with bioactive agents such as hydroxyapatite (HA). The work reported in this study demonstrates the direct 3D printing of PEEK/HA composites of up to 30 weight percent (wt%) HA using a Fused Filament Fabrication (FFF) approach. The surface characteristics and in vitro properties of the composite materials were investigated. X-ray diffraction revealed the samples to be semi-crystalline in nature, with X-ray Photoelectron Spectroscopy and Time-of-Flight Secondary Ion Mass Spectrometry revealing HA materials were available in the uppermost surface of all the 3D printed samples. In vitro testing of the samples at 7 days demonstrated that the PEEK/HA composite surfaces supported the adherence and growth of viable U-2 OS osteoblast like cells. These results demonstrate that FFF can deliver bioactive HA on the surface of PEEK bio-composites in a one-step 3D printing process.

scholarly journals Enzyme degradable star polymethacrylate/silica hybrid inks for 3D printing of tissue scaffolds

2020 ◽  
Vol 1 (9) ◽  
pp. 3189-3199
Author(s):  
Anna Li Volsi ◽  
Francesca Tallia ◽  
Haffsah Iqbal ◽  
Theoni K. Georgiou ◽  
Julian R. Jones
Keyword(s):  
3D Printing ◽  
Bone Regeneration ◽  
Tissue Scaffolds ◽  
Star Polymer ◽  
Polymer Architecture ◽  
Organic Hybrid ◽  
3D Printed ◽  
Silica Hybrid

We report the first enzyme cleavable inorganic–organic hybrid “inks” that can be 3D printed as scaffolds for bone regeneration and investigate the effect of star polymer architecture on their properties.

Scope and Challenges of 3D Printing in Organ Transplantation

2021 ◽  
Vol 07 ◽  
Author(s):  
Naman Shah ◽  
Sarthak Jain ◽  
Priyal Jain ◽  
Mamta Thakur
Keyword(s):  
3D Printing ◽  
Organ Transplantation ◽  
Organ Transplant ◽  
Review Article ◽  
Current Status ◽  
Technological Advancement ◽  
3D Printed ◽  
Scientists And Engineers ◽  
Insight Into

Background: The influx of 3D printing in organ transplantation is currently a vigorous area of research and its success is still cynical. The review article focuses mainly on the scope and challenges in the applications of 3D printing in organ transplantation. The basic idea of the article is to highlight the current status of 3D printing in the area of organ transplant. Introduction: The review article covers the highlights of 3D printing, major steps incurred in the 3D printing of organs, challenges in the 3D printing and transplantation of organs and future prospects (Scope) in the area with special reference to the problem and failures encountered in organ transplantation of 3D printed organs. Method: The findings from available studies have been consolidated in the review article to have an insight into the scope and challenges in the area of 3D printed organ transplantation. Result: In this review study, it has been found that there are certain limitations of the 3D printed material based on the survival and multiplication in the in-vivo environment, which subsequently leads to the bio incompatibility of the organs. In addition to this, some other limitations which provide further scope of research in this area are also included. Conclusion: It has been concluded that 3D printing is an emerging solution in organ transplantation and prosthetics, but still, more refinement and technological advancement is needed to make it a completely feasible solution. The joint team of doctors, scientists and engineers need to work cross disciplinary to overcome the limitations and to develop this technique further for the betterment of mankind.

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