The aerobic inhibition of glycolysis in pea-seed extracts and its possible relationship to the Pasteur effect

Simultaneous and continuous measurements of changes in CO2 and O2 concentrations in glucose-metabolizing yeast suspensions by mass spectrometry enabled a study of the Pasteur effect (aerobic inhibition of glycolysis) in Saccharomyces uvarum and Schizosaccharomyces pombe. A different control mechanism operates in Candida utilis to give a damped oscillation after the anaerobic-aerobic transition. The apparent Km values for respiration of the three yeasts were in the range 1.3-1.8 microM-O2. The apparent Km values for O2 of the Pasteur effect were 5 and 13 microM for catabolite-repressed and derepressed S. uvarum respectively and 7 microM for Sch. pombe. These results are discussed with respect to currently accepted mechanisms for the control of glycolysis.

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Evidence for proliferation of Enterobacter cloacae on carbohydrates in cucumber and pea spermosphere

Canadian Journal of Microbiology ◽

10.1139/m92-185 ◽

1992 ◽

Vol 38 (11) ◽

pp. 1128-1134 ◽

Cited By ~ 27

Author(s):

Daniel P. Roberts ◽

Carol J. Sheets ◽

John S. Hartung

Keyword(s):

Seed Storage ◽

Enterobacter Cloacae ◽

Resistant Mutant ◽

Loamy Sand Soil ◽

Key Words Carbohydrate ◽

Proliferation Assays ◽

Pea Seed ◽

Seed Extracts ◽

Storage Carbohydrates

Enterobacter cloacae EcCT501 grew on a variety of mono- and oligo-saccharides in vitro but did not grow on any of the polysaccharides tested. Carbohydrates found in seed exudates supported growth as did major monosaccharide constituents of seed storage carbohydrates and various seed-associated oligosaccharides. In addition, a rifampicin-resistant mutant of E. cloacae EcCT501, strain 501R3, grew on several of these mono- and oligo-saccharides in a natural gravely loamy sand soil. Glycosidase activities were detected in E. cloacae EcCT501 cultures that have the potential to degrade carbohydrate seed exudates and the oligosaccharide components of seed storage carbohydrates. Aqueous extracts from corn and pea seed induced activities of α-galactosidase 15- and 18-fold, respectively. Activities of α-glucosidase, β-glucosidase, and β-xylosidase were slightly induced by corn and pea seed extracts. Induction of these glycosidase activities by these extracts is consistent with their involvement in spermosphere proliferation. No carbohydrate depolymerase activities were detected. Strain A-11, which was isolated after mutation of strain 501R3 with transposon mini-Tn5 Km, lost the ability to grow on or had a reduced growth rate on several carbohydrates. Strain A-11 was similar to strain 501R3 in other nutritional tests. In cucumber spermosphere proliferation assays populations of strains 501R3 and A-11 were significantly different (P = 0.001) at 20 and 45 h. Strain A-11 did not proliferate in cucumber spermosphere, while strain 501R3 increased in number 21-fold after 45 h. In pea spermosphere, populations of strains A-11 and 501R3 increased 345- and 690-fold, respectively, after 45 h (significantly different at P = 0.1). These data are consistent with the hypothesis that spermosphere mono- and oligo-saccharides have roles as nutrients during spermosphere proliferation by E. cloacae. Key words: carbohydrate nutrition, spermosphere proliferation, glycosidase.

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A simple method of identifying peroxidase isoenzymes from crude pea seed extracts

Food Chemistry ◽

10.1016/0308-8146(88)90042-8 ◽

1988 ◽

Vol 29 (4) ◽

pp. 275-282 ◽

Cited By ~ 4

Author(s):

Helen C. Lee ◽

Barbara P. Klein

Keyword(s):

Peroxidase Isoenzymes ◽

Simple Method ◽

Pea Seed ◽

Seed Extracts

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Pyrophosphate and Fructose 2,6-Bisphosphate Effects on Glycolysis in Pea Seed Extracts

PLANT PHYSIOLOGY ◽

10.1104/pp.76.2.316 ◽

1984 ◽

Vol 76 (2) ◽

pp. 316-320 ◽

Cited By ~ 15

Author(s):

Douglas A. Smyth ◽

Min-Xian Wu ◽

Clanton C. Black

Keyword(s):

Pea Seed ◽

Seed Extracts

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The mechanism of the reversible aerobic inhibition of glycolysis in a pea-seed extract

Biochemical Journal ◽

10.1042/bj0750066 ◽

1960 ◽

Vol 75 (1) ◽

pp. 66-72 ◽

Cited By ~ 4

Author(s):

M. D. Hatch ◽

J. F. Turner

Keyword(s):

Seed Extract ◽

Inhibition Of Glycolysis ◽

Pea Seed

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Pea seed extracts stimulate germination of the terrestrial orchidOphrys apiferaHuds. during a habitat restoration project

Plant Biosystems - An International Journal Dealing with all Aspects of Plant Biology ◽

10.1080/11263504.2013.809814 ◽

2013 ◽

Vol 149 (1) ◽

pp. 54-60 ◽

Cited By ~ 3

Author(s):

Simon Pierce ◽

Valentina Guidi ◽

Andrea Ferrario ◽

Roberta M. Ceriani ◽

Massimo Labra ◽

...

Keyword(s):

Habitat Restoration ◽

Restoration Project ◽

Pea Seed ◽

Seed Extracts

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Exploitation of piscicidal potential of Anamirta cocculus (Linn.) seed extracts for the eradication of predatory wild fish Mystus vittatus (Bloch.)

International Journal of Scientific Research ◽

10.15373/22778179/apr2014/162 ◽

2012 ◽

Vol 3 (4) ◽

pp. 453-454

Author(s):

Qadir Umar ◽

◽

Muzaffer Umar

Keyword(s):

Wild Fish ◽

Mystus Vittatus ◽

Seed Extracts

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PENGARUH LAMA FERMENTASI TERHADAP KADAR FLAVONOID TOTAL PADA EKSTRAK TAPE BIJI NANGKA (Artocarpus heterophylla Lamk.)

Jurnal Kesehatan Kusuma Husada ◽

10.34035/jk.v12i1.552 ◽

2020 ◽

pp. 53-61

Author(s):

Nada Nabila Sari ◽

S Suharyanto

Keyword(s):

Extraction Method ◽

Positive Sample ◽

Total Flavonoid ◽

Test Results ◽

Fermentation Time ◽

Flavonoid Compounds ◽

Community People ◽

Qualitative Test ◽

Seed Extracts

Biji nangka kurang diminati oleh masyarakat sebagai bahan pangan. Biji nangka memiliki kandungan senyawa flavonoid yang dapat digunakan sebagai pengobatan. Tujuan peneliti adalah untuk mengetahui pengaruh lama fermentasi terhadap kadar flavonoid total pada ekstrak tape biji nangka (Artocarpus heterophylla Lamk.) yang dilakukan dengan alat spektrofotometer UV-Vis. Manfaat dari penelitian untuk menambah wawasan di bidang farmasi khususnya tentang ilmu kimia amami mengenai pengaruh lama fermentasi terhadap kadar flavonoid total pada ekstrak tape biji nangka (Artocarpus heterophylla Lamk.). Metode ekstraksi yang digunakan adalah metode maserasi dengan mengunakan pelarut etanol 96%. Identifikasi kualitatif mengunakan uji Shinoda, uji NaOH 10%, uji H2SO4 (pekat). Hasil uji kualitatif menunjukan sampel positif mengandung flavonoid. Kesimpulan pada hasil penelitian penetapan kadar flavonoid total yang terkandung dalam ekstrak tape biji nangka pada hari ke-0 sebesar 2,9146±0,0245 mgQE/gram ekstrak, pada hari ke-1 sebesar 3,3050±0,0240 mgQE/gram ekstrak, pada hari ke-2 sebesar 3,6829±0,0244 mgQE/gram ekstrak, dan pada hari ke-3 sebesar 4,0488±0,0244 mgQE/gram ekstrak. Jackfruit seeds are less interest by the community (people) as food. Jackfruit seeds containing flavonoid compounds where it can be used as a treatment. The aim of the researchers was to determine the effect of fermentation time on total flavonoid concentration in jackfruit fermented seed extracts with a UV-Vis spectrophotometer. The benefits of research to add insight in the field of pharmacy especially about chemistry of amami regarding effect of fermentation time on total flavonoid levels in jackfruit seed “tape” extract (Artocarpus heterophylla Lamk.). Extraction method used is the maceration method using 96% ethanol solvent. Qualitative identification using the Shinoda test, 10% NaOH test, and H2SO4 (concentrated) test. Qualitative test results showed a positive sample containing flavonoids. Conclusions on the results of the experiment are jackfruit seed "tape" extract on day-0 was 2.9146±0.0245 mgQE/gram extract, on day-1 was 3.3050±0.0240 mgQE/gram extract, on day-2 was 3.68293±0.0244 mgQE/gram and on day-3 was 4.0488±0.024 mgQE /gram extract. The longer fermentation, total flavonoid concentration interest.

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