scholarly journals Free amino acids of testes. Concentrations of free amino acids in the testes of several species and the precursors of glutamate and glutamine in rat testes in vivo

1973 ◽  
Vol 132 (3) ◽  
pp. 353-359 ◽  
Author(s):  
Isa K. Mushahwar ◽  
Roger E. Koeppe
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Guinea Pig ◽  
Free Amino Acids ◽  
Free Amino ◽  
Ammonium Acetate ◽  
Testicular Tissue ◽  
Species Variation

Determination of the free amino acid and lactate content of testicular tissue in rat, guinea pig, rabbit, cat, gerbil, hamster, chicken and bullfrog indicates a substantial species variation. Insulin hypoglycaemia and ammonium acetate toxicity changes the concentration of several free amino acids of rat testes. 14C radioactivity from labelled acetate and ethanol is rapidly incorporated into some of the free amino acids of rat testes in vivo, whereas incorporation from [14C]glucose is relatively slow. These results have been compared with those obtained from similar studies with rat brain. In contrast to brain, there is no evidence for glutamate compartmentation in testes.

Correlation between osmoregulation and cell volume regulation

1987 ◽  
Vol 252 (4) ◽  
pp. R768-R773
Author(s):  
M. A. Lang
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Osmotic Pressure ◽  
Cell Volume ◽  
Free Amino Acids ◽  
Volume Regulation ◽  
Free Amino ◽  
Cell Volume Regulation

The euryhaline crab, Callinectes sapidus, behaves both as an osmoregulator when equilibrated in salines in the range of 800 mosM and below and an osmoconformer when equilibrated in salines above 800 mosM. There exists a close correlation between osmoregulation seen in the whole animal in vivo and cell volume regulation studied in vitro. Hyperregulation of the hemolymph osmotic pressure and cell volume regulation both occurred in salines at approximately 800 mosM and below. During long-term equilibration of the crabs to a wide range of saline environments, the total concentration of hemolymph amino acids plus taurine remained below 3 mM. During the first 6 h after an acute osmotic stress to the whole animal, the hemolymph osmotic pressure and Na activity gradually decreased, whereas the free amino acids remained below 3 mM. As the hemolymph osmotic pressure decreased below approximately 850 mosM, the amino acid level began to increase to 17-25 mM. This change was primarily due to increases in glycine, proline, taurine, and alanine. The likely source of the increase in hemolymph free amino acids in vivo is the free amino acid loss from muscle cells observed during cell volume regulation in vitro.

scholarly journals Determination of free amino acid and biogenic amine contents of hungarian sparkling wines

2004 ◽  
Vol 22 (SI - Chem. Reactions in Foods V) ◽  
pp. S287-S289 ◽  
Author(s):  
L. Simon-Sarkadi ◽  
E. Szőke ◽  
A. Kerekes
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Biogenic Amines ◽  
Free Amino Acid ◽  
Biogenic Amine ◽  
Free Amino Acids ◽  
Free Amino ◽  
Exchange Chromatography ◽  
Sparkling Wine

Comparative study was conducted on the basis of free amino acids and biogenic amines of Hungarian sparkling wines originated from 3 producers (Törley, Hungária, Balaton Boglár). Determination of amino acids and biogenic amines was accomplished by ion-exchange chromatography using an amino acid analyser. The dominant free amino acids in sparkling wines were proline and arginine and the major biogenic amine was spermidine. Based on results of chemometric analyses, free amino acid and biogenic amine contents seemed to be closely related to quality and the technology of sparkling wine making.

Quantitative determination of free amino acids with automatic amino acid analyser in liver cirrhosis blood plasma

1966 ◽  
Vol 1 (3) ◽  
pp. 75-75
Author(s):  
S. Tanaka ◽  
K. Hattori ◽  
Y. Katoo ◽  
S. Suga ◽  
A. Ishiwara ◽  
...  
Keyword(s):  
Amino Acids ◽  
Liver Cirrhosis ◽  
Amino Acid ◽  
Quantitative Determination ◽  
Blood Plasma ◽  
Free Amino Acids ◽  
Free Amino ◽  
Amino Acid Analyser

Comparison of procedures for extracting free amino acids from polymorphonuclear lekocytes.

1976 ◽  
Vol 22 (10) ◽  
pp. 1618-1622 ◽  
Author(s):  
Y Houpert ◽  
P Tarallo ◽  
G Siest
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Quantitative Determination ◽  
Free Amino Acids ◽  
Free Amino ◽  
Polymorphonuclear Leukocytes ◽  
Extraction Procedure ◽  
Intracellular Pool ◽  
Human Polymorphonuclear Leukocytes

Abstract We studies five methods for extracting amino acids from human polymorphonuclear leukocytes. Both the use of cell lysis and of a deproteinizing agent interfere with quantitative determination of the amino acids, basic amino acids being the most sensitive to the extraction procedure. Among the methods used, disruption of the cells by freezing-thawing is the best method for extracting all the amino acids. Taurine is the only amino acid extracted in the same amount by all the methods studied, and it represents half of the intracellular pool.

Determination of free amino acids and 18 elements in freeze-dried strawberry and blueberry fruit using an Amino Acid Analyzer and ICP-MS with micro-wave digestion

2014 ◽  
Vol 147 ◽  
pp. 189-194 ◽  
Author(s):  
Hua Zhang ◽  
Zhen-Yu Wang ◽  
Xin Yang ◽  
Hai-Tian Zhao ◽  
Ying-Chun Zhang ◽  
...  
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Free Amino Acids ◽  
Free Amino ◽  
Icp Ms ◽  
Freeze Dried ◽  
Amino Acid Analyzer

Determination of Amino Acid Needs of the Young Pig by Nitrogen Balance and Plasma-Free Amino Acids

1968 ◽  
Vol 27 (5) ◽  
pp. 1327 ◽  
Author(s):  
J. R. Mitchell ◽  
D. E. Becker ◽  
A. H. Jensen ◽  
B. G. Harmon ◽  
H. W. Norton
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Nitrogen Balance ◽  
Free Amino Acids ◽  
Free Amino ◽  
Plasma Free Amino Acids

The amino acid requirements of the rediae of Cryptocotyle lingua and Himasthla leptosoma and of the sporocyst of Cercaria emasculans Pelseneer, 1900

Parasitology ◽  
1970 ◽  
Vol 61 (3) ◽  
pp. 491-496 ◽  
Author(s):  
Stuart D. M. Watts
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Free Amino Acids ◽  
Free Amino ◽  
Food Sources ◽  
Amino Acid Requirement ◽  
Molar Ratios ◽  
Amino Acid Requirements ◽  
Free Amino Acid Pools

Few workers have studied the amino acid requirement of larval Digenea in vivo. Cheng (1963) conducted a study of three species of parasites, each from a different host, and suggested that both free and bound host amino acids act as food sources. His assumptions are based on the disappearance of free amino acids from the sera of infected molluscs and the qualitative similarities between parasite and host with respect to both free and bound amino acids. Negus (1968), working on Turritella communis infected with the sporocysts of Cercaria doricha, described an almost identical qualitative composition of both the free amino acid pools and the hydrolysates of host gonad and parasite tissue. He was of the opinion that this was not coincidental. Considerable quantitative similarities between the free amino acids were also evident. Read, Rothman & Simmons (1963), in discussing cestode metabolism and membrane transport, proposed that it is the molar ratios of amino acids in the host which are important to the parasite and that the amino acid requirement may be more subtle than a simple need for certain components. Indeed, they suggest that this may form one basis for physiological host specificity.

Intestinal Absorption of Glycine and Glycyl-L-Proline in the Rat

1977 ◽  
Vol 52 (6) ◽  
pp. 607-614 ◽  
Author(s):  
R. C. Heading ◽  
H. P. Schedl ◽  
L. D. Stegink ◽  
D. L. Miller
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Intestinal Absorption ◽  
Free Amino Acid ◽  
Brush Border ◽  
Free Amino Acids ◽  
Free Amino ◽  
Mucosal Tissue ◽  
Tissue Samples

1. Absorption of [3H]glycine and [14C]-glycyl-l-proline at concentrations between 0·5 and 4 mmol/l was studied by perfusion in vivo of rat jejunal and ileal segments. Absorption was defined as net removal of radioisotope from the perfusate. Radioactivity assays and amino acid analyses were performed on perfusates and on mucosal tissue samples obtained from the perfused segments. 2. At the concentrations studied, absorption rates of glycine and glycylproline were proportional to concentration. Ileal rates were approximately 60% of jejunal rates. Glycylproline absorption was slightly faster than glycine absorption and was associated with the appearance in the perfusate of free glycine and proline in the ratio approximately 3:1. Intraluminal hydrolysis was insufficient to account for the amounts of free amino acids found and glycylproline hydrolase activity at the brush border is known to be minimal. 3. Glycylproline absorption apparently occurred by transport of the intact peptide followed by its intracellular hydrolysis. However, more than one-third of absorbed glycine and one-eighth of absorbed proline returned to the lumen as free amino acid. This reflux of glycine and proline was not proportional to their concentrations in the mucosa.

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