scholarly journals Some properties of cellobiose oxidase from the white-rot fungus Sporotrichum pulverulentum

1985 ◽  
Vol 228 (3) ◽  
pp. 557-564 ◽  
Author(s):  
F F Morpeth
Keyword(s):  
Superoxide Anion ◽  
Preliminary Investigation ◽  
Substrate Inhibition ◽  
White Rot ◽  
Sedimentation Equilibrium ◽  
White Rot Fungus ◽  
Optical Fluorescence ◽  
Sporotrichum Pulverulentum ◽  
High Concentrations ◽  
Prosthetic Groups

Cellobiose oxidase from the white-rot fungus Sporotrichum pulverulentum has been purified to homogeneity by a new procedure. The carbohydrate and amino acid compositions of the enzyme have been determined. Cellobiose oxidase contains FAD and cytochrome b prosthetic groups. Mr of the enzyme has been estimated at 74400 by sedimentation equilibrium. The enzyme is a monomer. Optical, fluorescence and e.p.r. spectra of oxidized and reduced cellobiose oxidase have been determined. A preliminary investigation of the substrate specificity of cellobiose oxidase reveals that disaccharides and even some insoluble polysaccharides are substrates, but not monosaccharides. Strong substrate inhibition is seen at high concentrations of cellobiose. This effect is particularly marked when oxygen is the electron acceptor. Cellobiose oxidase is unusual among flavoproteins, since it stabilizes the red anionic flavin semiquinone and forms a sulphite adduct, yet appears to produce the superoxide anion as its primary reduced oxygen product.

scholarly journals Resolution, purification and some properties of the multiple forms of cellobiose quinone dehydrogenase from the white-rot fungus Sporotrichum pulverulentum

1986 ◽  
Vol 236 (1) ◽  
pp. 221-226 ◽  
Author(s):  
F F Morpeth ◽  
G D Jones
Keyword(s):  
Superoxide Anion ◽  
Sugar Content ◽  
White Rot ◽  
Sedimentation Equilibrium ◽  
White Rot Fungus ◽  
Neutral Sugar ◽  
Apparent Difference ◽  
Multiple Forms ◽  
Sporotrichum Pulverulentum ◽  
Similar Kinetic

Four forms of cellobiose quinone dehydrogenase have been purified from the white-rot fungus Sporotrichum pulverulentum. The Mr of the enzyme has been estimated by sedimentation equilibrium to be 57,800 and by SDS/polyacrylamide-gel to be 60,000. These enzymes are clearly monomers. Cellobiose quinone dehydrogenases contain FAD and variable amounts of a green chromophore which we suggest is 6-hydroxy-FAD. The superoxide anion and H2O2 are the products of its reaction with oxygen. All of the isoenzymes from any one preparation display similar kinetic parameters. However, these vary between preparations. The only apparent difference between the four separable isoenzymes is their neutral-sugar content.

Metabolism of trans-ferulic acid by the white-rot fungus sporotrichum pulverulentum

1981 ◽  
Vol 128 (4) ◽  
pp. 349-354 ◽  
Author(s):  
Jugal K. Gupta ◽  
Sven G. Hamp ◽  
John A. Buswell ◽  
Karl-Erik Eriksson
Keyword(s):  
Ferulic Acid ◽  
White Rot ◽  
White Rot Fungus ◽  
Sporotrichum Pulverulentum

Vanillic acid metabolism by the white-rot fungus Sporotrichum pulverulentum

1980 ◽  
Vol 125 (3) ◽  
pp. 189-202 ◽  
Author(s):  
Paul Ander ◽  
Annele Hatakka ◽  
Karl-Erik Eriksson
Keyword(s):  
Vanillic Acid ◽  
Acid Metabolism ◽  
White Rot ◽  
White Rot Fungus ◽  
Sporotrichum Pulverulentum

scholarly journals Chemical composition and natural durability of juvenile and mature heartwood of Robinia pseudoacacia L.

2011 ◽  
Vol 83 (3) ◽  
pp. 1059-1068 ◽  
Author(s):  
João V.F Latorraca ◽  
Oliver Dünisch ◽  
Gerald Koch
Keyword(s):  
Cell Walls ◽  
Robinia Pseudoacacia ◽  
Brown Rot ◽  
White Rot ◽  
White Rot Fungus ◽  
Natural Durability ◽  
Forest Sites ◽  
High Concentrations ◽  
Robinia Pseudoacacia L ◽  
Heartwood Extractives

The aim of this study was to characterize the properties of juvenile and mature heartwood of Robinia pseudoacacia L. (black locust). The content, the composition, and subcellular localization of heartwood extractives were studied in 14 old-grown trees from forest sites in Germany and Hungary, as well as in 16 younger trees of four clone types. Heartwood extractives (methanol and acetone extraction) were analysed by HPLC-chromatography. UV microspectrophotometry was used to localize the extractives in the wood cell walls. The natural durability of juvenile and mature heartwood was analysed according to the European standard EN 350-1. Growth analyses, as well as the chemical analyses, showed that in Robinia the formation of juvenile wood is restricted to the first 10-15 years of cambial growth. In the heartwood high contents of phenolic compounds and flavonoids were present, which were in high concentrations in the cell walls of the axial parenchyma and of the vessels. In the juvenile heartwood, the content of these extractives is significantly lower than in the mature heartwood. In agree, the juvenile heartwood had a lower resistance to decay by Coniophora puteana (brown rot fungus) and Coriolus versicolor (white rot fungus) compared to the mature.

scholarly journals Kinetics of inter-domain electron transfer in flavocytochrome cellobiose dehydrogenase from the white-rot fungus Phanerochaete chrysosporium

2002 ◽  
Vol 365 (2) ◽  
pp. 521-526 ◽  
Author(s):  
Kiyohiko IGARASHI ◽  
Ikuo MOMOHARA ◽  
Takeshi NISHINO ◽  
Masahiro SAMEJIMA
Keyword(s):  
Electron Transfer ◽  
Phanerochaete Chrysosporium ◽  
Substrate Concentration ◽  
Substrate Inhibition ◽  
Cellobiose Dehydrogenase ◽  
White Rot ◽  
White Rot Fungus ◽  
Isosbestic Point ◽  
Second Phase ◽  
Kinetics Of

The pre-steady-state kinetics of inter-domain electron transfer in the extracellular flavocytochrome cellobiose dehydrogenase from Phanerochaete chrysosporium was studied using various values of pH and substrate concentration. Monitoring at the isosbestic point of each prosthetic group indicated that the reductive half-reactions of flavin and haem were biphasic and monophasic respectively. When the observed rates of the flavin and haem reactions were plotted against substrate concentration, the behaviour of the second phase of the flavin reduction was almost identical with that of haem reduction at all substrate concentrations and pH values tested, suggesting that the formation of flavin semiquinone and haem reduction involve the same electron transfer reaction. Although flavin reduction by cellobiose was observed in the range of pH3.0–7.0, the velocity of the next electron transfer step decreased with increase of pH and was almost zero above pH6.0. The second phase of flavin reduction and the haem reduction were inhibited similarly by high concentrations of the substrate, whereas the first phase of flavin reduction showed a hyperbolic relation to the cellobiose concentration. Increase in pH enhanced the substrate inhibition of haem reduction but not the initial flavin reduction. Moreover, the dissociation constant Kd of flavin reduction and the substrate inhibition constant Ki of haem reduction decreased similarly with an increase of pH. From these results, it is evident that binding of cellobiose to the active site inhibits electron transfer from flavin to haem.

scholarly journals Statistical Optimization of Conditions for Decolorization of Synthetic Dyes byCordyceps militarisMTCC 3936 Using RSM

2015 ◽  
Vol 2015 ◽  
pp. 1-17 ◽  
Author(s):  
Baljinder Kaur ◽  
Balvir Kumar ◽  
Neena Garg ◽  
Navneet Kaur
Keyword(s):  
Incubation Time ◽  
Liquid Culture ◽  
Ph Value ◽  
Preliminary Investigation ◽  
White Rot ◽  
White Rot Fungus ◽  
Synthetic Dyes ◽  
Culture Systems ◽  
Reactive Green 19 ◽  
Optimized Conditions

In the present study, the biobleaching potential of white rot fungusCordyceps militarisMTCC3936 was investigated. For preliminary screening, decolorization properties ofC. militariswere comparatively studied using whole cells in agar-based and liquid culture systems. Preliminary investigation in liquid culture systems revealed 100% decolorization achieved within 3 days of incubation for reactive yellow 18, 6 days for reactive red 31, 7 days for reactive black 8, and 11 days for reactive green 19 and reactive red 74. RSM was further used to study the effect of three independent variables such as pH, incubation time, and concentration of dye on decolorization properties of cell free supernatant ofC. militaris. RSM based statistical analysis revealed that dye decolorization by cell free supernatants ofC. militarisis more efficient than whole cell based system. The optimized conditions for decolorization of synthetic dyes were identified as dye concentration of 300 ppm, incubation time of 48 h, and optimal pH value as 5.5, except for reactive red 31 (for which the model was nonsignificant). The maximum dye decolorizations achieved under optimized conditions for reactive yellow 18, reactive green 19, reactive red 74, and reactive black 8 were 73.07, 65.36, 55.37, and 68.59%, respectively.

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