The chemical mechanism of sheep liver 6-phosphogluconate dehydrogenase. A Schiff-base intermediate is not involved
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[2-18O]Ribulose 5-phosphate was prepared and shown to be converted enzymically by 6-phosphogluconate dehydrogenase from sheep liver into 6-phosphogluconate with complete retention of the heavy isotope. This finding unequivocally excludes the possibility of a Schiff-base mechanism for the enzyme. The involvement of metal ions has already been excluded, and other possible mechanisms are discussed. The enzyme was purified by an improved large-scale procedure, which is briefly described.
1999 ◽
Vol 32
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pp. 241-284
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1973 ◽
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pp. 623-635
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2007 ◽
Vol 72
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pp. 908-916
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2021 ◽
Vol 1046
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pp. 012006
2021 ◽
Vol 790
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pp. 012031
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2002 ◽
Vol 320
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pp. 237-247
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