HPLC-MS/MS Quantitative Determination of Gallic acid and Cyanidin-3-Glucoside Content of Bilberry Fruit Extract from Turkey

Planta Medica ◽  
2011 ◽  
Vol 77 (12) ◽  
Author(s):  
N Kırımer ◽  
F Göger ◽  
KHC Başer
Keyword(s):  
Quantitative Determination ◽  
Gallic Acid ◽  
Fruit Extract

Quantitative Determination of Gallic acid and Cyanidin-3-O-Glucoside within Sumac Extracts by HPLC-MS/MS

Planta Medica ◽  
2011 ◽  
Vol 77 (12) ◽  
Author(s):  
M Koşar ◽  
F Göger ◽  
N Kırımer ◽  
KHC Başer
Keyword(s):  
Quantitative Determination ◽  
Gallic Acid

scholarly journals QUANTITATIVE DETERMINATION OF PHENOL CARBOXYLIC ACIDS IN POMEGRANATE FRUIT PULP BY THE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY WITH ULTRAVIOLET DETECTION

2019 ◽  
Vol 12 (1) ◽  
pp. 548
Author(s):  
Ruben Ashotovich Pogosyan ◽  
Olga Vladimirovna Nesterova ◽  
Dmitry Olegovich Bokov ◽  
Irina Alexandrovna Samylina
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Phenolic Compounds ◽  
Quantitative Determination ◽  
Gallic Acid ◽  
Carboxylic Acids ◽  
High Performance ◽  
Fruit Pulp ◽  
Pomegranate Fruit

Objective: Pomegranate (Punica granatum L.) is a broadly used plant possessing a wide range of medicinal properties. In this research, we have mainly focused on the investigation of phenolic compounds of pomegranate fruit pulp (PFP).Methods: Fresh fruits of “Çəhrayı Gülöyşə,” “Kizil-anor,” and pomegranate varietal mixture were used as samples. High-performance liquid chromatography-ultraviolet (HPLC-UV) analysis of phenol carboxylic acids was performed with metal column Kromasil® C18 (4.6×250 mm, particle size 5 μm) and the acetonitrile-water-concentrated acid phosphoric system (400:600:5) under isocratic elution conditions (flow rate of 0.5 ml/min). Detection was carried out using a UV detector “GILSTON” UV/Visible model 151 at a wavelength of 280 nm.Results and Discussion: As a result of our research, we proposed chromatographic conditions for the separation of phenolic compounds, the conditions for sample preparation of PFP. Procedure for determination of phenolic carboxylic acids total content in terms of gallic acid by HPLC-UV method was developed. According to the obtained data, the content of phenolic carboxylic acids should be at least 0.7%.Conclusion: Procedure for the quantitative determination of gallic acid using the HPLC-UV method was developed. This method which can be used in the standardization of new medicinal plant raw materials - PFP, as well as extract preparations based on it in the future.

scholarly journals QUANTITATIVE DETERMINATION OF PHENOL CARBOXYLIC ACIDS IN POMEGRANATE FRUIT PULP BY THE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY WITH ULTRAVIOLET DETECTION

2019 ◽  
Vol 12 (1) ◽  
pp. 548
Author(s):  
Ruben Ashotovich Pogosyan ◽  
Olga Vladimirovna Nesterova ◽  
Dmitry Olegovich Bokov ◽  
Irina Alexandrovna Samylina
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Phenolic Compounds ◽  
Quantitative Determination ◽  
Gallic Acid ◽  
Carboxylic Acids ◽  
High Performance ◽  
Fruit Pulp ◽  
Pomegranate Fruit

Objective: Pomegranate (Punica granatum L.) is a broadly used plant possessing a wide range of medicinal properties. In this research, we have mainly focused on the investigation of phenolic compounds of pomegranate fruit pulp (PFP).Methods: Fresh fruits of “Çəhrayı Gülöyşə,” “Kizil-anor,” and pomegranate varietal mixture were used as samples. High-performance liquid chromatography-ultraviolet (HPLC-UV) analysis of phenol carboxylic acids was performed with metal column Kromasil® C18 (4.6×250 mm, particle size 5 μm) and the acetonitrile-water-concentrated acid phosphoric system (400:600:5) under isocratic elution conditions (flow rate of 0.5 ml/min). Detection was carried out using a UV detector “GILSTON” UV/Visible model 151 at a wavelength of 280 nm.Results and Discussion: As a result of our research, we proposed chromatographic conditions for the separation of phenolic compounds, the conditions for sample preparation of PFP. Procedure for determination of phenolic carboxylic acids total content in terms of gallic acid by HPLC-UV method was developed. According to the obtained data, the content of phenolic carboxylic acids should be at least 0.7%.Conclusion: Procedure for the quantitative determination of gallic acid using the HPLC-UV method was developed. This method which can be used in the standardization of new medicinal plant raw materials - PFP, as well as extract preparations based on it in the future.

scholarly journals HPTLC Fingerprint Profile (Phenolics) of Selected Cyathea Species from Western Ghats, South India

2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Janakiraman Narayanan ◽  
Johnson Marimuthu alias Antonysamy
Keyword(s):  
Quality Control ◽  
Quantitative Determination ◽  
Gallic Acid ◽  
Western Ghats ◽  
South India ◽  
Rapid Screening ◽  
Ethanolic Extracts

HPTLC analysis was performed to study the phenolics, flavonoids, and tannins profile of Cyathea nilgirensis Holttum, Cyathea gigantea (Wall. ex. Hook.) Holttum, and Cyathea crinita (Hook.) Copel. The maximum number of bands was observed in the ethanolic extracts of C. crinita. The phenolic bands with Rf values 0.05, 0.32, 0.43, 0.75 (quercetin), and 0.83 demonstrated their occurrence in all the three studied species of Cyathea. Flavonoids with Rf values 0.05, 0.23, 0.30, 0.43, and 0.73 showed their presence in the three studied species of Cyathea. Tannins with the Rf values 0.05, 0.09, and 0.82 determined their existence in all the three studied Cyathea species. Gallic acid was present only in C. crinita with the Rf value 0.49. The developed HPTLC profiles can be used for identification and quantitative determination of phenolics, flavonoids, and tannins. This method is also suitable for rapid screening of Cyathea species for chemotypic assessment and also for quality control purposes.

scholarly journals Quantitative determination of total Phenolic and Tannin contents of Murraya koenigii Twigs

2021 ◽  
Vol 23 (09) ◽  
pp. 432-438
Author(s):  
Shruthi Shree Gandhi SGandhi SGandhi S ◽  
◽  
Dr.Dhinek .A ◽  
Keyword(s):  
Electron Transfer ◽  
Quantitative Determination ◽  
Gallic Acid ◽  
Reduction Reaction ◽  
Total Phenolic ◽  
Single Electron Transfer ◽  
Tannin Content ◽  
Murraya Koenigii ◽  
Ethanolic Extracts

The total phenolic and tannin content present in the ethanolic extracts of Murrayakoenigii twigs was estimated in this study. The ethanolic extracts of Murrayakoenigii twigs phenolic and tannin contents were measured using Folin-Ciocalteu’s method. Folin-Ciocalteu’s method. Works on the basis of oxidation and reduction reaction with single electron transfer with gallic acid and tannic acid taken as standards. The study revealed the presence of a significant amount of phenolic and tannin content in the ethanolic extracts of Murrayakoenigii twigs.

scholarly journals Визначення кількісного вмісту суми фенольних сполукв артишоку суцвіттях, часнику листі та цибулинах

2018 ◽  
Author(s):  
A. I. Fedosov ◽  
V. S. Kyslychenko ◽  
O. M. Novosel
Keyword(s):  
Phenolic Compounds ◽  
Quantitative Determination ◽  
Gallic Acid ◽  
Plant Species ◽  
Medical Practice ◽  
Wide Distribution ◽  
Biologically Active ◽  
Active Compounds ◽  
Quantitative Content

Introduction. Phenolic compounds are the most abundant class of biologically active compounds of herbal origin. They possess antioxidant, anti-tumor, antimutagenic, antisclerotic, antibacterial, antiviral, sedative, anti-ulcer and anti-inflammatory activity. Wide distribution of phenolic compounds in the plant world and their usage in medical practice provide the importance of their quantitative determination in prospective plant species, such as artichoke and garlic.The purpose of current work was determination of quantitative content of phenolic compounds in artichoke inflorescences, garlic leaves and bulbs.Methods of research. The content of phenolic compounds was determined using various methods: permanganatometric, spectrophotometric calculated on gallic acid, spectrophotometric according to the SPU method.Results and discussion. The content of the sum of phenolic compounds, determined by permanganatometry, comprised 9,74% in artichoke inflorescences, 8,96% in garlic leaves and 4,85% – in garlic bulbs; determined spectrophotometrically calculated on gallic acid in artichoke inflorescences was 6,58%, in garlic leaves – 6,22%, in garlic bulbs – 2,87%; determined spectrophotometrically according to the SPU method in artichoke inflorescences comprised 4,43%, in garlic leaves – 3,91%, and in garlic bulbs – 2,11%.Conclusions.The quantitative content of phenolic compounds in artichoke, garlic leaves and bulbs was determined using various methods: permanganatometric, spectrophotometric calculated on gallic acid, spectrophotometric according to the SPU method.As a result of the experiment, it was found that phenolic compounds accumulate in the artichoke inflorescences and garlic leaves, and in garlic bulbs their content is lower.The results obtained allow the recommending artichoke inflorescences, garlic leaves and bulbs as the herbal sources of phenolic compounds.

scholarly journals QUANTITATIVE DETERMINATION OF THE SUM OF PHENOLIC COMPOUNDS IN FRUIT OF RHUS TYPHINA(L.)

2019 ◽  
pp. 225-232
Author(s):  
Виктория (Viktoria) Нодарьевна (Nodar'evna) Леонова (Leonova) ◽  
Иван (Ivan) Викторович (Viktorovich) Попов (Popov) ◽  
Ольга (Ol'ga) Ивановна (Ivanovna) Попова (Popova) ◽  
Владимир (Vladimir) Павлович (Pavlovich) Зайцев (Zaitsev)
Keyword(s):  
Phenolic Compounds ◽  
Quantitative Determination ◽  
Gallic Acid ◽  
Calibration Graph ◽  
Water Extraction ◽  
Complexation Reaction ◽  
Alcohol Water ◽  
Relative Standard ◽  
Rhus Typhina

The genus Rhus of the Anacardiaceae family includes about 120–150 species of small trees, bushes, and lianas. The chemical composition of the sumac fluffy Rhus typhina (L.) is almost unknown. Previously, some phenolic compounds (rutin, quercetin, hyperoside, tannin, gallic acid) were identified in the fruits of the plant. To quantify the amount of phenolic compounds in the raw materials studied, a method based on the complexation reaction of phenolic compounds with a phosphomolybdic-tungsten reagent (Folin-Denis reagent) in an alkaline medium was chosen. The optimal conditions for the complexation reaction are selected: the ratio of the solution of gallic acid and Denis-Folin reagent is 0.06 mg / 1.6 ml, respectively. For the quantitative determination of the amount of phenolic compounds for the aqueous extraction of sumac fluffy must be added 1.1 ml of Denis-Folin reagent, to alcohol-water extraction – 1.2 ml of Folin-Denis reagent. The maximum value of optical density in aqueous and alcohol-aqueous extractions is observed at a wavelength of 720 nm. In alcohol-water extraction, more phenolic compounds (A = 0.8683) pass into the alcoholic water than to the aqueous one (A = 0.6346). The method used is valid and can be used for quantitative determination of the amount of phenolic compounds in fruits of sumac fluffy. To evaluate the linearity, the regression equation is calculated y = 941.67x + 0.0725 and the correlation coefficient r = 0.9895. The concentration of phenolic compounds in alcohol-water extraction was determined from the calibration graph. The average content of the sum of the phenolic compounds in terms of the gallic acid in the alcohol- water extraction was 10.04 ± 0.17%. The relative standard deviation (RSD) is 2.61%.

scholarly journals Identification and quantitative determination of arbutin in the herb of Orthilia secunda

2021 ◽  
Vol 10 (4) ◽  
pp. 122-128
Author(s):  
A. V. Lezina ◽  
I. I. Terninko ◽  
M. V. Krysko
Keyword(s):  
Medicinal Plant ◽  
Quantitative Determination ◽  
Gallic Acid ◽  
Hplc Analysis ◽  
Spectral Characteristics ◽  
Array Detector ◽  
Hydrolytic Cleavage ◽  
Anti Inflammatory ◽  
Identification And Quantification

Introduction. Orthilia secunda (L.) House is a perennial herb that grows in Europe, Siberia, Asia Minor and Central Asia. The herb of Orthilia secunda is actively used in folk medicine as a diuretic, wound-healing and anti-inflammatory agent. From literary sources it is known that this medicinal plant raw material (PRM) contains flavonoids, tannins, organic acids, vitamins, as well as simple phenols and their derivatives (arbutin and hydroquinone). The presence of arbutin is responsible for the plant's high antioxidant and anti-inflammatory properties. But the use of Orthilia secunda in official medicine is limited due to the lack of complete information on the chemical composition and criteria for standardization of this type of medicinal product.Aim. Identification and quantification of arbutin by chromatographic methods in Orthilia secunda (L.) House, harvested in various phytocenotic zones.Materials and methods. The investigated medicinal plant material – the herb of Orthilia secunda – was harvested in various phytocenotic zones: in July 2018, harvesting was carried out in the northern part of Kazakhstan (Kokshetau district), in July-August 2019 in the Perm Territory and in the Tyumen Region. Preliminary identification of arbutin and related phenols – gallic acid and hydroquinone – was carried out by high performance thin layer chromatography (HPTLC) on a CAMAG instrument with a UV cabinet (Merck HPTLC silica gel 60 F154 plates, 20 × 10), semi-automatic Linomat 5 applicator (sample application). Elution of the plates was performed in a CAMAG Automatic Developing Chamber (ADC2). Image fixation was performed on a CAMAG Scanner 3 spectrodensitometer. The quantitative determination of arbutin was carried out by the method of highperformance liquid chromatography, which was carried out on a Prominence LC-20 device (Shimadzu, Japan) according to the validated method described in the European Pharmacopoeia 10.0. Diode array detector SPD-M20A, column Intersil C18 column (250–4.6 mm, 5 μm) (Phenomenex, USA). The results were processed using the LabSolution software. The identification and quantification of arbutin was carried out in comparison with a standard solution containing a reference sample (RS) of arbutin (C = 0,025 mg/ml) and RS of hydroquinone (C = 0,0125 mg/ml).Results and discussion. HPTLC analysis made it possible to detect arbutin and gallic acid – the main product of hydrolytic degradation/ precursor of the biosynthesis of tannins of the hydrolysable group – in the herb of Orthilia secunda from different places of growth. HPLC analysis demonstrates a different chromatographic profile of Orthilia herb harvested in different phytocenotic zones. However, in all studied objects, the absence of hydroquinone and the presence of substances that can presumably be attributed to its derivatives were confirmed, which is confirmed by the visual similarity of the spectra of these compounds and the proximity of the extrema. It was found that arbutin does not belong to the marker (majority) compounds of Orthilia. Its content is low and reaches a maximum (about 0,021 %) in the herb of Orthilia secunda growing on the territory of Kazakhstan, while in the herb of Orthilia harvested in the Perm Territory arbutin was not identified. From the data obtained, it follows that the greatest accumulation of arbutin occurs in areas with a warmer and drier climate (northern part of Kazakhstan).Conclusion. HPTLC analysis of the herb Orthilia secunda allowed the identification of arbutin and gallic acid (the main precursor of tannins of the hydrolysable group). The results of HPLC analysis of Orthilia herb harvested in various phytocenotic zones suggest quantitative differences in the content of arbutin depending on the region of growth. From the experimental data, it follows that Orthilia growing in the northern part of Kazakhstan accumulates the maximum (0,021%) amount of arbutin, in comparison with the samples harvested in the Tyumen region and the Perm region. At the same time, Orthilia harvested in the Perm Territory does not accumulate arbutin. The presence of hydroquinone has not been confirmed (by HPTLC and HPLC methods); therefore, it is not justified to talk about the hydrolytic cleavage of arbutin in the process of biosynthesis or drying. However, in all studied objects there are peaks of substances with spectral characteristics like hydroquinone, which makes it possible to assume the presence of its derivatives. Therefore, it is not advisable to position arbutin as a marker compound of Orthilia secunda harvested on the territory of the Russian Federation, and to standardize raw materials for this compound.

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