Bioactivity in Australian native willow: comparative analysis of leaf extracts on cell viability

2017 ◽  
Author(s):  
A Patil ◽  
M Fitzgerald ◽  
N Shaw Paul ◽  
MO Parat
Keyword(s):  
Comparative Analysis ◽  
Cell Viability ◽  
Leaf Extracts

Comparative analysis of different groups of phenolic compounds in fruit and leaf extracts of Aronia sp.: A. melanocarpa, A. arbutifolia, and A. ×prunifolia and their antioxidant activities

2017 ◽  
Vol 243 (9) ◽  
pp. 1645-1657 ◽  
Author(s):  
Agnieszka Szopa ◽  
Adam Kokotkiewicz ◽  
Paweł Kubica ◽  
Piotr Banaszczak ◽  
Agnieszka Wojtanowska-Krośniak ◽  
...  
Keyword(s):  
Comparative Analysis ◽  
Phenolic Compounds ◽  
Antioxidant Activities ◽  
Leaf Extracts

scholarly journals PROTECTIVE EFFECT OF CASSIA FISTULA EXTRACTS AGAINST ULTRAVIOLET C INJURY IN HUMAN CORNEAL EPITHELIAL CELLS

2019 ◽  
pp. 261-266
Author(s):  
DEEKSHA K ◽  
CYNTHIA ARUNACHALAM
Keyword(s):  
Antioxidant Activity ◽  
Protective Effect ◽  
Cell Viability ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Cassia Fistula ◽  
Leaf Extracts ◽  
Corneal Epithelial ◽  
Ultraviolet C ◽  
Uvc Radiation

Objective: The objective of this study was to investigate the antioxidant activity of Indian laburnum (Cassia fistula L. [CF]) leaf extracts and their impact on ultraviolet C (UVC) radiation-induced damage on human corneal epithelial (HCE) cells. Methods: The antioxidant activity and free radical scavenging ability of CF leaf extracts were determined by in vitro methods such as 1,1-diphenyl- 2-picrylhydrazyl radical scavenging capacity, Total antioxidant capacity (TAC), and reducing power. The total phenolic content (TPC), total flavonoid content (TFC), and preliminary phytochemical screening were done to ensure the pharmacological effects of the extracts. The UVC radiation at wavelength 254 nm was used to irradiate HCE cells and cell viability was assessed by methyl thiazolyl tetrazolium assay. Results: Extracts at the concentration of 200 μg/ml did not affect the cell viability of HCE cells. Almost 50% cell death was observed after UVC irradiation at a dose of 200 J/m2. Both extracts showed a protective effect by increasing the cell viability of irradiated cells up to 57.28% and 62.39%. A dose-dependent increase in the TAC and reducing power of the extract was observed. The TPC in aqueous and ethanol extracts of CF leaves was 18.8 and 27.80 mg gallic acid equivalent/g sample while TFC was 8.47 and 16.5 mg quercetin equivalent per/gsample, respectively. Conclusions: CF leaves are a potent source of bioactive compounds with good antioxidant potential. Exposure to UVC radiation cause harmful effects on HCE cells and the extracts have shown to have potent protective effects on UV light-induced oxidative stress in HCE cells.

scholarly journals Ginkgo biloba L. Leaf Extract Protects HepG2 Cells Against Paraquat-Induced Oxidative DNA Damage

Plants ◽  
2019 ◽  
Vol 8 (12) ◽  
pp. 556 ◽  
Author(s):  
Amélia M. Silva ◽  
Sandra C. Silva ◽  
Jorge P. Soares ◽  
Carlos Martins-Gomes ◽  
João Paulo Teixeira ◽  
...  
Keyword(s):  
Dna Damage ◽  
Oxidative Damage ◽  
Cell Viability ◽  
Ginkgo Biloba ◽  
Hepg2 Cells ◽  
Leaf Extract ◽  
Oxidative Dna Damage ◽  
Leaf Extracts ◽  
Ginkgo Biloba Leaf Extract ◽  
Ginkgo Biloba Leaf

Ginkgo biloba L. leaf extracts and herbal infusions are used worldwide due to the health benefits that are attributed to its use, including anti-neoplastic, anti-aging, neuro-protection, antioxidant and others. The aim of this study was to evaluate the effect of an aqueous Ginkgo biloba extract on HepG2 cell viability, genotoxicity and DNA protection against paraquat-induced oxidative damage. Exposure to paraquat (PQ), over 24 h incubation at 1.0 and 1.5 µM, did not significantly reduce cell viability but induced concentration and time-dependent oxidative DNA damage. Ginkgo biloba leaf extract produced dose-dependent cytotoxicity (IC50 = 540.8 ± 40.5 µg/mL at 24 h exposure), and short incubations (1 h) produced basal and oxidative DNA damage (>750 and 1500 µg/mL, respectively). However, lower concentrations (e.g., 75 µg/mL) of Ginkgo biloba leaf extract were not cytotoxic and reduced basal DNA damage, indicating a protective effect at incubations up to 4 h. On the other hand, longer incubations (24 h) induced oxidative DNA damage. Co-incubation of HepG2 cells for 4 h, with G. biloba leaf extract (75 µg/mL) and PQ (1.0 or 1.5 µM) significantly reduced PQ-induced oxidative DNA damage. In conclusion, the consumption of Ginkgo biloba leaf extract for long periods at high doses/concentrations is potentially toxic; however, low doses protect the cells against basal oxidative damage and against environmentally derived toxicants that induce oxidative DNA damage.

scholarly journals Comparative Analysis of Dynamic Cell Viability, Migration and Invasion Assessments by Novel Real-Time Technology and Classic Endpoint Assays

PLoS ONE ◽  
2012 ◽  
Vol 7 (10) ◽  
pp. e46536 ◽  
Author(s):  
Ridha Limame ◽  
An Wouters ◽  
Bea Pauwels ◽  
Erik Fransen ◽  
Marc Peeters ◽  
...  
Keyword(s):  
Comparative Analysis ◽  
Cell Viability ◽  
Real Time ◽  
Migration And Invasion ◽  
Dynamic Cell

scholarly journals Moringa Oleifera Leaf Extracts Protect BMSC Osteogenic Induction Following Peroxidative Damage by Activating The PI3K/Akt /Foxo1 Pathway

2020 ◽  
Author(s):  
Meiling Liu ◽  
Haifeng Ding ◽  
Hongzhi Wang ◽  
Manfeng Wang ◽  
Xiaowei Wu ◽  
...  
Keyword(s):  
Cell Viability ◽  
Osteogenic Differentiation ◽  
Moringa Oleifera ◽  
Caspase 3 ◽  
Therapeutic Effects ◽  
Mrna Levels ◽  
Leaf Extracts ◽  
Alizarin Red ◽  
Peroxidative Damage ◽  
Osteogenic Induction

Abstract Objective: We aimed to investigate the therapeutic effects of Moringa oleifera leaf extracts on osteogenic induction of rat bone marrow mesenchymal stem cells (BMSCs) following peroxidative damage and to explore the underlying mechanisms. Methods: Conditioned medium was used to induce osteogenic differentiation of BMSCs, which were treated with H2O2, Moringa oleifera leaf extracts-containing serum, or the phosphatidyl inositol-3 kinase (PI3K) inhibitor Wortmannin, alone or in combination. Cell viability was measured using the MTT assay. Cell cycle was assayed using flow cytometry. Expression levels of Akt, phosphorylated (p)Akt, Foxo1, and cleaved caspase-3 were analyzed using Western blot analysis. The mRNA levels of osteogenesis-associated genes, including alkaline phosphatase (ALP), collagen І, osteopontin (OPN), and Runx2, were detected using qRT-PCR. Reactive oxygen species (ROS) and malondialdehyde (MDA) levels, as well as superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), and ALP activity were detected using commercially available kits. Osteogenic differentiation capability was determined using alizarin red staining. Results: During osteogenic induction of rat BMSCs, H2O2 reduced cell viability and proliferation, inhibited osteogenesis, increased ROS and MDA levels, and decreased SOD and GSH-PX activity. H2O2 significantly reduced pAkt and Foxo1 expression, and increased cleaved caspase-3 levels in BMSCs. Additional treatments with Moringa oleifera leaf extracts partially reversed the H2O2-induced changes. Wortmannin partially attenuated the effects of Moringa oleifera leaf extracts on protein expression of Foxo1, pAkt, and cleaved caspase-3, as well as mRNA levels of osteogenesis-associated genes.Conclusion: Moringa oleifera leaf extracts ameliorate peroxidative damage and enhance osteogenic induction of rat BMSCs by activating the PI3K/Akt/Foxo1 pathway.

scholarly journals INHIBITION OF LIPID ACCUMULATION IN 3T3-L1 ADIPOCYTES BY MORINDA CITRIFOLIA LINN. LEAF EXTRACTS AND COMMERCIAL HERBAL FORMULAS FOR WEIGHT CONTROL

2016 ◽  
Vol 8 (12) ◽  
pp. 199
Author(s):  
Aurasorn Saraphanchotiwitthaya ◽  
Pattana Sripalakit
Keyword(s):  
Cell Viability ◽  
Dose Response ◽  
Lipid Accumulation ◽  
Leaf Extract ◽  
Colorimetric Method ◽  
Morinda Citrifolia ◽  
Leaf Extracts ◽  
Fat Accumulation ◽  
Triglyceride Content

<p><strong>Objective: </strong>To determine the <em>in vitro</em> anti-obesity effects of <em>Morinda citrifolia</em> leaf extract and herbal formulas used for weight loss in Thailand on lipid accumulation in 3T3-L1 adipocytes.</p><p><strong>Methods: </strong>Differentiated 3T3-L1 adipocytes were treated with 0.1, 0.5 and 1 mg/ml <em>M. citrifolia</em> leaf extract, three herbal formulas (JL-RU, JL-TH, CD-H) and 1, 5 and 10 mg/ml rutin, gallic acid and caffeine. Lipid accumulation determined by measuring Oil Red O staining and triglyceride content measured by a colorimetric method in adipocytes were assayed compared to the control. The effect of test samples on the viability of preadipocytes and differentiated adipocytes were investigated.<strong></strong></p><p><strong>Results: </strong>Differentiated adipocytes treated with 1 mg/ml <em>M. citrifolia</em> extract moderately inhibited fat accumulation (45.12%) and highly reduced triglyceride content (85.09%). Among the three herbal formulas, JL-TH considerably inhibited fat accumulation (109.17%, 1 mg/ml) and decreased triglyceride content (95.00%, 1 mg/ml) in adipocytes; this was higher than that for CD-H and JL-RU, respectively. The viability of preadipocytes treated with CD-H at 1 mg/ml was slightly decreased while those treated with JL-TH at 0.05-1 mg/ml showed moderately decreased viability in a dose-response manner. For differentiated adipocytes, CD-H at 0.5-1 mg/ml moderately decreased cell viability while JL-TH at 0.05-1 mg/ml caused moderate to high reduction of cell viability in a dose-response relationship.</p><p><strong>Conclusion: </strong><em>M. citrifolia</em> extract and three herbal formulas had anti-obesity effects in 3T3-L1 adipocytes, as indicated by a significant reduction in lipid accumulation, triglyceride content, and cell viability. These findings suggest a potential therapeutic approach for the prevention and treatment of obesity.</p>

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