REGULATION OF INTERLEUKIN-2 (IL-2) PRODUCTION BY ENDOTHELIAL CELL (EC) DERIVED SUBSTANCES
We recently reported that vascular EC support the staphylococcal protein A (SPA)-induced production of IL-2 by T cells, and furthermore do so synergistically with monocytes. We have now assessed the contributions of EC membrane-associated and secreted factors to these functions. IL-2 was measured by either human tonsil blast of CTLL cell assay. Separation of EC from T cells by a permeable membrane (.45μ pore size) prevented IL-2 production. Consistent with this, supernatant from resting EC (ECs) was also unable to induce IL-2 generation. In addition, neither a crude EC plasma membrane preparation nor paraformaldehyde-fixed EC supported IL-2 production. The combination of fixed EC + ECs likewise did not reconstitute the accessory cell role of intact EC. This was not due to the absence of class II MHC antigens, since EC which were fixed after incubation with interferon (to induce HLA-DR expression) were also unable to promote IL-2. However, the addition of ECs to cultures of T + live EC enhanced IL-2 production by 42% (mean, n=7). This effect did not require the presence of detectable IL-1 in the ECs, and was not reproduced by purified IL-1. When added to unfractionated peripheral blood mononuclear cells (PBM), both fixed EC and ECs enhanced IL-2 production (increments of 120 ± 37% and 115 ± 44% respectively). This effect was dose related, and peaked at 20% ECs by volume. Incubation of EC with calcium ionophore (10™6 M) yielded a supernatant (ECs-i) with greatly enhanced IL-2 promoting activity despite the fact that ionophore alone suppressed IL-2: lllu for T + EC + ECs-i vs. 7u for T + EC + ECs + ionophore. Therefore, substances secreted by EC and presented on the EC surface are able to augment mitogen induced IL-2 production by T cells in the presence of accessory cells. The EC-derived mediator is apparently distinct from IL-1. EC may function in concert with monocytes as important immune regulatory cells at the blood/tissue interface.