Identification of Platelet Antigens by Immunoprecipitation of Unlabelled Platelet Glycoproteins

1990 ◽  
Vol 64 (03) ◽  
pp. 469-472 ◽  
Author(s):  
Werner Stenziger ◽  
Beate Kehrel ◽  
Jürgen van de Loo
Keyword(s):  
Monoclonal Antibodies ◽  
Silver Staining ◽  
Protein G ◽  
Time Saving ◽  
Radioactive Substances ◽  
Reducing Conditions ◽  
Fast Flow ◽  
Murine Monoclonal Antibodies ◽  
Sensitive Method

SummaryA time-saving and sensitive method for the identification of antigens on unlabelled platelet glycoproteins (GP) based on immunoprecipitation has been developed. Platelet solubilisates were incubated with antibodies to form GP-antibody complexes that were precipitated with Protein G Sepharose 4 Fast Flow (PGS). Pcs-bound material was eluted, separated by SDSPAGE under reducing conditions and visualized by silver staining. The method was designed as an alternative to radioimmunoprecipitation for laboratories not equipped to work with radioactive substances. It is proposed as a complementary procedure for the characterization of platelet GP antigens by murine monoclonal antibodies and human alloantibodies.

scholarly journals Production and characterization of monoclonal antibodies to the edta extract of Leptospira interrogans, serovar icterohaemorrhagiae

1996 ◽  
Vol 29 (5) ◽  
pp. 483-489
Author(s):  
Lilian Terezinha de Queiroz Leite ◽  
Mauricio Resende ◽  
Wanderley de Souza ◽  
Elizabeth R.S. Camargos ◽  
Matilde Cota Koury
Keyword(s):  
Monoclonal Antibody ◽  
Monoclonal Antibodies ◽  
Western Blot ◽  
Silver Staining ◽  
Colloidal Gold ◽  
Leptospira Interrogans ◽  
Outer Envelope ◽  
Lethal Challenge ◽  
Sds Page

Monoclonal antibodies (MABs) ivere produced against an etbylenediaminetetraacetate (EDTA) extract of Leptospira interrogans serovar icterohaemorrhagiae being characterized by gel precipitation as IgM and IgG (IgGl and IgG2b). The EDTA extract was detected as several bands by silver staining in SDS-PAGE. In the Western blot the bands around 20 KDa reacted with a monoclonal antibody, 47B4D6, and was oxidized by periodate and was not digested by pronase, suggesting that the determinant is of carbohydrate nature, lmmunocytochemistry, using colloidal gold labeling, showed that an EDTA extract determinant recognized by monoclonal antibody 47B4D6, is localized under the outer envelope of serovar icterohaemorrhagiae. Hoe AIAB raised against the EDTA extract was not able to protect hamsters from lethal challenge with virulent homologous leptospires.

Generation and Characterization of Murine Monoclonal Antibodies anti-PBP2a of Methicillin-resistantStaphylococcus aureus

2015 ◽  
Vol 34 (4) ◽  
pp. 257-262 ◽  
Author(s):  
José Procópio M. Senna ◽  
Maria da Glória M. Teixeira ◽  
Marta de A. Santiago ◽  
Nádia M. Batoréu ◽  
Napoleão Valadares ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Murine Monoclonal Antibodies

Characterization of Murine Monoclonal Antibodies to HIV-1 Induced by Synthetic Peptides

1988 ◽  
Vol 4 (5) ◽  
pp. 331-342 ◽  
Author(s):  
PAUL J. DURDA ◽  
BARBARA LEECE ◽  
ANNA JENOSKI ◽  
HARVEY RABIN ◽  
AMANDA FISHER ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Synthetic Peptides ◽  
Murine Monoclonal Antibodies ◽  
Hiv 1

Characterization of Novel Murine Monoclonal Antibodies Directed Against the Extracellular Domain of Human HER2 Tyrosine Kinase Receptor

Hybridoma ◽  
2011 ◽  
Vol 30 (4) ◽  
pp. 347-353 ◽  
Author(s):  
Tohid Kazemi ◽  
Fathollah Tahmasebi ◽  
Ali Ahmad Bayat ◽  
Neda Mohajer ◽  
Jalal Khoshnoodi ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Tyrosine Kinase ◽  
Extracellular Domain ◽  
Tyrosine Kinase Receptor ◽  
Murine Monoclonal Antibodies

scholarly journals Production and characterization of murine monoclonal antibodies to Histoplasma capsulatum yeast cell antigens.

1989 ◽  
Vol 57 (3) ◽  
pp. 896-901 ◽  
Author(s):  
S M Kamel ◽  
L J Wheat ◽  
M L Garten ◽  
M S Bartlett ◽  
M R Tansey ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Yeast Cell ◽  
Histoplasma Capsulatum ◽  
Murine Monoclonal Antibodies

Characterization of new murine monoclonal antibodies directed against glycophorins C and D

1994 ◽  
Vol 4 (2) ◽  
pp. 147-155 ◽  
Author(s):  
M. J. Loirat ◽  
W. Dahr ◽  
J. Y. Muller ◽  
D. Blanchard
Keyword(s):  
Monoclonal Antibodies ◽  
Murine Monoclonal Antibodies

Characterization of murine monoclonal antibodies against serogroup B salmonellae and application as serotyping reagents.

1991 ◽  
Vol 29 (9) ◽  
pp. 1899-1903 ◽  
Author(s):  
R S Tsang ◽  
K H Chan ◽  
N W Lau ◽  
D K Choi ◽  
D K Law ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Murine Monoclonal Antibodies

Generation and Characterization of a Murine Monoclonal Antibody Specific for the Human T1-Cd5 Molecule

1987 ◽  
Vol 2 (3) ◽  
pp. 143-150 ◽  
Author(s):  
Federico Genzano ◽  
Ada Funaro ◽  
Massimo Alessio ◽  
Lucia B. De Monte ◽  
Graziella Bellone ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Monoclonal Antibodies ◽  
T Cell ◽  
T Lymphocytes ◽  
Membrane Glycoprotein ◽  
Functional Features ◽  
Specific Membrane ◽  
Murine Monoclonal Antibodies ◽  
Selection Of

Murine monoclonal antibodies (MoAbs) have found widespread applications in the characterization of the molecular and functional features of lymphocyte differentiation antigens. The present paper summarizes the results of our work dealing with the production and selection of a murine MoAb recognizing a molecule expressed during the whole differentiative life of T lymphocytes. The MoAb CB01 resulted to be specific for an apparently unique epitope of the T-cell specific membrane glycoprotein T1-CD5.

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