Automated Monitoring of Platelet Aggregation in Multiple Samples of whole Blood
Whole blood may be first agitated in a circular transparent rotor chamber, and then rotated continuously to produce plasma/red cell separation for monitoring. The rotor is driven by a low inertia, printed circuit d.c. motor (108 Watt) which is automatically controlled. The pattern of rotation is determined by a signal generator which provides various waveforms at adjustable frequencies and also at fixed speeds.By segmenting the rotor into multiple cuvettes (Vol = 0.4 ml) it is possible to monitor the platelet concentration, after agitation, as each cuvette spins through the stationary optical system. This consists of a light-emitting diode (peak: 900 nm), pulsed at 10 kHz in order to eliminate ambient light effects, and a photodetector with a built-in amplifier, fed to an amplifying circuit which eliminates other signals. An electronic circuit permits selection of the signal from each cuvette on a direct reading meter. Cuvette No. 1 is identified by a photocell-detector trigger.Platelet-free plasma is about zero O. D. in the infra red with respect to a water blank, and with attention to specific criteria such as optical geometry and light path, platelet concentration may be accurately determined.