Isolation of Four Ecdysones from Ajuga iva Roots and a Rapid Semiquantitative Method for Ecdysone Determination

Planta Medica ◽  
1981 ◽  
Vol 42 (07) ◽  
pp. 293-295 ◽  
Author(s):  
N. Sabri ◽  
A. Asaad ◽  
S. Khafagy
Keyword(s):  
Semiquantitative Method

LANDSLIDE SUSCEPTIBILITY ANALYSIS BASED ON A SEMIQUANTITATIVE METHOD IN THE SIERRA-COSTA REGION, MICHOACÁN, MEXICO

2021 ◽  
pp. 1-24
Author(s):  
Karla Aurora De La Peña Guillen ◽  
Manuel E. Mendoza ◽  
José Luis Macías ◽  
Berenice Solis-Castillo
Keyword(s):  
Landslide Susceptibility ◽  
Susceptibility Analysis ◽  
Semiquantitative Method

Real-Time PCR Detection of Ruminant DNA

2004 ◽  
Vol 67 (3) ◽  
pp. 550-554 ◽  
Author(s):  
LUIS MENDOZA-ROMERO ◽  
EDWARD L. C. VERKAAR ◽  
PAUL H. SAVELKOUL ◽  
ARNOLD CATSBURG ◽  
HENK J. M. AARTS ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Bovine Spongiform Encephalopathy ◽  
Real Time ◽  
Real Time Pcr ◽  
Spongiform Encephalopathy ◽  
Meat And Bone Meal ◽  
Bone Meal ◽  
Dna Targeting ◽  
Species Origin ◽  
Semiquantitative Method

To control the spread of bovine spongiform encephalopathy, several DNA methods have been described for the detection of the species origin of meat and bone meal. Most of these methods are based on the amplification of a mitochondrial DNA segment. We have developed a semiquantitative method based on real-time PCR for detection of ruminant DNA, targeting an 88-bp segment of the ruminant short interspersed nuclear element Bov-A2. This method is specific for ruminants and is able to detect as little as 10 fg of bovine DNA. Autoclaving decreased the amount of detectable DNA, but positive signals were observed in feeding stuff containing 10% bovine material if this had not been rendered in accordance with the regulations, i.e., heated at 134°C for 3 instead of 20 min.

Vital microscopy of islet blood flow: catecholamine effects in normal and ob/ob mice

1987 ◽  
Vol 252 (1) ◽  
pp. E130-E135 ◽  
Author(s):  
P. Rooth ◽  
I. B. Taljedal
Keyword(s):  
Blood Flow ◽  
Capillary Flow ◽  
Inhibitory Influence ◽  
Video Recordings ◽  
Islet Blood Flow ◽  
Visible Effect ◽  
Flow Through ◽  
Vital Microscopy ◽  
Semiquantitative Method ◽  
Normal Controls

The pancreatic microcirculation in noninbred ob/ob mice and normal controls was visualized in a fluorescence microscope and examined for responses to intravenous infusions of epinephrine or norepinephrine. Evaluations of coded video recordings revealed a smooth dose-response relationship and validated a semiquantitative method of analysis. In ob/ob mice the islet microcirculation was markedly and reversibly inhibited by 0.14–4 micrograms of epinephrine X min-1 X kg body wt-1; the flow was almost totally stopped at the highest infusion rates. Capillary flow in the exocrine pancreas appeared unaffected, except for some inhibition at 4.0 micrograms X min-1 X kg-1. Norepinephrine was less potent an inhibitor of islet blood flow. Normal lean mice exhibited minor responses to 1–11.3 micrograms of epinephrine X min-1 X kg-1; in most cases there was no visible effect. It is concluded that there is a selective regulation of blood flow through the endocrine portion of the pancreas and that the islet microcirculation is hypersensitive to catecholamines in noninbred ob/ob mice. A defective inhibitory influence from the brain may play a role in the development of excessive hyperinsulinemia in ob/ob mice.

Prevalence of Vertebral Fractures by Semiautomated Morphometry in Patients with Ankylosing Spondylitis

2011 ◽  
Vol 38 (5) ◽  
pp. 893-897 ◽  
Author(s):  
NURIA MONTALA ◽  
XAVIER JUANOLA ◽  
EDUARDO COLLANTES ◽  
ELISA MUÑOZ-GOMARIZ ◽  
CARLOS GONZALEZ ◽  
...  
Keyword(s):  
New York ◽  
Ankylosing Spondylitis ◽  
Vertebral Fractures ◽  
Axial Skeleton ◽  
Chronic Inflammatory Disease ◽  
National Registry ◽  
Prevalent Vertebral Fracture ◽  
High Prevalence ◽  
Prevalent Fracture ◽  
Semiquantitative Method

Objective.Ankylosing spondylitis (AS) is a chronic inflammatory disease mainly affecting the axial skeleton and characterized by ossification of the spinal disc, joints, and ligaments leading to progressive ankylosis. Vertebral osteoporosis is a recognized feature of AS. Studies have confirmed a moderate to high prevalence of vertebral fractures with extremely varying ranges in patients with AS. Our objective was to estimate the prevalence of vertebral fractures in a representative Spanish population of patients with AS using a validated semiquantitative method, MorphoXpress®.Methods.Patients were randomly selected from the 10 initial participating centers of the Spanish National Registry of Spondyloarthropathies (REGISPONSER) by consecutive sampling. All patients fulfilled the New York modified criteria for AS and had a baseline thoracolumbar radiograph. A prevalent vertebral fracture was defined according to the Genant classification criteria.Results.The estimated prevalence of vertebral fractures was 32.4% (95% CI 25.5%–39.3%). The majority of fractures were localized in the thoracic segment (n = 100; 82.%) and were mild (n = 79; 64.8%). In logistic regression analysis, age (odds ratio per year 1.05, 95% CI 1.03–1.08, p < 0.001), disease duration (OR per year 1.03, 95% CI 1.01–1.06, p = 0.011), Bath Ankylosing Spondylitis Functional Index score (OR per score 1.16, 95% CI 1.03–1.30, p = 0.015), Bath Ankylosing Spondylitis Radiographic Index-TS (OR per score 1.25, 95% CI 1.12–1.39, p < 0.001), and wall-occiput distance (OR per cm 1.15, 95% CI 1.08–1.23, p < 0.001) were all associated with prevalent fracture.Conclusion.Semiquantitative methods are needed to improve the diagnosis of vertebral fractures in AS in order to start early treatment and to avoid complications arising from osteoporosis.

Prognostic significance of architectural subtypes of Gleason grade 4 prostate cancer in radical prostatectomy: A semiquantitative method of evaluation

2021 ◽  
Vol 50 ◽  
pp. 151678
Author(s):  
Alexandre R. da Paz ◽  
Athanase Billis ◽  
Leandro L.L. Freitas ◽  
Larissa B.E. Costa ◽  
Icleia S. Barreto ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Radical Prostatectomy ◽  
Prognostic Significance ◽  
Gleason Grade ◽  
Method Of Evaluation ◽  
Semiquantitative Method

Dynamics of spectral components of heart rate variability during changes in autonomic balance

1998 ◽  
Vol 275 (1) ◽  
pp. H213-H219 ◽  
Author(s):  
Michael V. Højgaard ◽  
Niels-Henrik Holstein-Rathlou ◽  
Erik Agner ◽  
Jørgen K. Kanters
Keyword(s):  
Heart Rate ◽  
Heart Rate Variability ◽  
Supine Position ◽  
Low Frequency ◽  
Power Spectral Analysis ◽  
Frequency Domain Analysis ◽  
Autonomic Balance ◽  
Power Spectral ◽  
Normalized Units ◽  
Semiquantitative Method

Frequency domain analysis of heart rate variability (HRV) has been proposed as a semiquantitative method for assessing activities in the autonomic nervous system. We examined whether absolute powers, normalized powers, and the low frequency-to-high frequency ratio (LF/HF) derived from the HRV power spectrum could detect shifts in autonomic balance in a setting with low sympathetic nervous tone. Healthy subjects were examined for 3 h in the supine position during 1) control conditions ( n = 12), 2) acute β-blockade ( n = 11), and 3) chronic β-blockade ( n = 10). Heart rate fell during the first 40 min of the control session (72 ± 2 to 64 ± 2 beats/min; P < 0.005) and was even lower during acute and chronic β-blockade (56 ± 2 beats/min; P < 0.005). The powers of all spectral areas rose during the first 60 min in all three settings, more so with β-blockade ( P < 0.05). LF/HF was found to contain the same information as powers expressed in normalized units. LF/HF detected the shift in autonomic balance induced by β-blockade but not the change induced by supine position. In conclusion, none of the investigated measures derived from power spectral analysis comprehensively and consistently described the changes in autonomic balance.

A SEMIQUANTITATIVE METHOD FOR THE DETECTION OF ACETIC ANHYDRIDE IN ACETIC ACID

1949 ◽  
Vol 27f (6) ◽  
pp. 266-268 ◽  
Author(s):  
G. Benson ◽  
R. M. Kitchen
Keyword(s):  
Corrosion Resistance ◽  
Acetic Acid ◽  
Acetic Anhydride ◽  
Colorimetric Test ◽  
Semiquantitative Method

When acetic acid is handled in aluminum, it is important, for reasons of corrosion resistance, that the acid be not completely anhydrous, a condition that results if traces of acetic anhydride are present. A quick colorimetric test depending on the reaction between acetic anhydride with aniline has been developed by which it is possible to detect 0.05% acetic anhydride in acetic acid.

Rapid, semi-quantitative assay of C-reactive protein evaluated

1991 ◽  
Vol 37 (11) ◽  
pp. 1981-1982 ◽  
Author(s):  
H Vallance ◽  
G Lockitch
Keyword(s):  
Preterm Infants ◽  
Screening Test ◽  
Quantitative Method ◽  
Comparison Method ◽  
Quantitative Assay ◽  
Plasma Samples ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Nephelometric Method ◽  
Semiquantitative Method

Abstract We evaluated a new rapid semi-quantitative immunometric assay of C-reactive protein (CRP) as a screening test for sepsis by comparison with an automated nephelometric method. Plasma samples (n = 101) from preterm infants during the first week of life were saved for CRP analyses. We measured CRP by the Nycocard semiquantitative method and compared the results with those obtained with a Behring Nephelometer. A CRP value less than 10 mg/L was considered to be negative for infection. All CRP results read as less than 10 mg/L (negative) by the Nycocard method were also less than 10 mg/L by the comparison method, and all CRP values found to be greater than 20 mg/L (positive) by the Nycocard method were also positive by the comparison method. Results in the 10-20 mg/L range were considered equivocal. We conclude that the Nycocard CRP semi-quantitative method is a rapid and useful screening test for sepsis in preterm infants.

Sign in / Sign up

Export Citation Format

Share Document