scholarly journals Thermoelastic study of nanolayered structures using time-resolved X-ray diffraction at high repetition rate

2014 ◽  
Vol 104 (2) ◽  
pp. 021906 ◽  
Author(s):  
H. A. Navirian ◽  
D. Schick ◽  
P. Gaal ◽  
W. Leitenberger ◽  
R. Shayduk ◽  
...  
Keyword(s):  
Repetition Rate ◽  
High Repetition Rate ◽  
X Ray Diffraction ◽  
Time Resolved ◽  
X Ray ◽  
High Repetition

scholarly journals Observation of substrate diffusion and ligand binding in enzyme crystals using high-repetition-rate mix-and-inject serial crystallography

IUCrJ ◽  
2021 ◽  
Vol 8 (6) ◽  
Author(s):  
Suraj Pandey ◽  
George Calvey ◽  
Andrea M. Katz ◽  
Tek Narsingh Malla ◽  
Faisal H. M. Koua ◽  
...  
Keyword(s):  
Repetition Rate ◽  
Initial Phase ◽  
High Repetition Rate ◽  
Irreversible Inhibitor ◽  
Time Resolved ◽  
X Ray ◽  
High Repetition ◽  
Substrate Diffusion ◽  
Crystal Volume

Here, we illustrate what happens inside the catalytic cleft of an enzyme when substrate or ligand binds on single-millisecond timescales. The initial phase of the enzymatic cycle is observed with near-atomic resolution using the most advanced X-ray source currently available: the European XFEL (EuXFEL). The high repetition rate of the EuXFEL combined with our mix-and-inject technology enables the initial phase of ceftriaxone binding to the Mycobacterium tuberculosis β-lactamase to be followed using time-resolved crystallography in real time. It is shown how a diffusion coefficient in enzyme crystals can be derived directly from the X-ray data, enabling the determination of ligand and enzyme–ligand concentrations at any position in the crystal volume as a function of time. In addition, the structure of the irreversible inhibitor sulbactam bound to the enzyme at a 66 ms time delay after mixing is described. This demonstrates that the EuXFEL can be used as an important tool for biomedically relevant research.

scholarly journals High Repetition Rate and Coherent Free-Electron Laser Oscillator in the Tender X-ray Range Tailored for Linear Spectroscopy

2021 ◽  
Vol 11 (13) ◽  
pp. 5892
Author(s):  
Michele Opromolla ◽  
Alberto Bacci ◽  
Marcello Rossetti Conti ◽  
Andrea Renato Rossi ◽  
Giorgio Rossi ◽  
...  
Keyword(s):  
Free Electron ◽  
Repetition Rate ◽  
Free Electron Laser ◽  
High Repetition Rate ◽  
Laser Oscillator ◽  
Time Resolved ◽  
X Ray ◽  
High Repetition ◽  
Super Conducting ◽  
Fine Time

Fine time-resolved analysis of matter—that is, spectroscopy and photon scattering—in the linear response regime requires fs-scale pulsed, high repetition rate, fully coherent X-ray sources. A seeded Free-Electron Laser, driven by a linac based on Super Conducting cavities, generating 108–1010 coherent photons at 2–5 keV with 0.2–1 MHz of repetition rate, can address this need. The scheme proposed is a Free-Electron Laser Oscillator at 3 keV, working with a cavity based on X-ray mirrors. The whole chain of the X-ray generation is here described by means of start-to-end simulations.

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