scholarly journals The Localization of Acid Phosphatase in the Sieve Element of Pisum

1969 ◽  
Vol 22 (4) ◽  
pp. 1051 ◽  
Author(s):  
S-Y Zee
Keyword(s):  
Electron Microscopy ◽  
Acid Phosphatase ◽  
Light Microscopy ◽  
Phosphatase Activity ◽  
Acid Phosphatase Activity ◽  
Lead Nitrate ◽  
Sieve Tube ◽  
Sieve Element ◽  
Recent Advances ◽  
Acid Phosphatase Reaction

Acid phosphatase activity has been detected within the sieve-tube members of plants by many workers using the Gomori technique and light microscopy (Lester and Evert 1965; Flinn and Smith 1967). Unfortunately the limited resolution makes it difficult to determine the specific sites of activity of the reaction product of the enzyme; recent advances in histochemical techniques for electron microscopy have made it possible to investigate more specifically the sites of localization of the acid phosphatase reaction product by using the Gomori lead nitrate technique (Goldfischer, Essner, and Novikoff 1964; Catesson and Czanenski 1967; Bowen 1968; Figier 1968; Wardrop 1968).

Acid-phosphatase activity in sieve-tube members of Tilia americana

Planta ◽  
1965 ◽  
Vol 65 (2) ◽  
pp. 180-185 ◽  
Author(s):  
Hope H. Lester ◽  
Ray F. Evert
Keyword(s):  
Acid Phosphatase ◽  
Phosphatase Activity ◽  
Acid Phosphatase Activity ◽  
Sieve Tube

Localisation cytochimique des activités phosphatasiques acides de champignons mycorhiziens développés sur milieu complet ou carencé en phosphate

1983 ◽  
Vol 61 (5) ◽  
pp. 1411-1414 ◽  
Author(s):  
Bernadette Lacaze
Keyword(s):  
Electron Microscopy ◽  
Enzyme Activity ◽  
Acid Phosphatase ◽  
Phosphatase Activity ◽  
Cell Walls ◽  
Acid Phosphatase Activity ◽  
Inorganic Phosphate ◽  
Light And Electron Microscopy ◽  
Reaction Products ◽  
Cytochemical Study

The mycelia of three mycorrhizal basidiomycètes (Pisolithus tinctorius (Pers.) Coker et Couch., Suillus granulatus (L. ex Fr.) O. Kuntze and S. bellinii (Izenga) Watling) were grown on media with or without inorganic phosphate. A cytochemical study of the distribution of acid phosphatase activity was made using light and electron microscopy. Highly enhanced enzyme activity was observed in the phosphorus-deficient mycelia. Precipitates were located primarily at the surface of the fungal cells. Cell walls appear devoid of reaction products in most cases.

scholarly journals CENTRIFUGAL, BIOCHEMICAL, AND ELECTRON MICROSCOPIC ANALYSIS OF CYTOPLASMIC PARTICULATES IN LIVER HOMOGENATES

1956 ◽  
Vol 2 (1) ◽  
pp. 33-54 ◽  
Author(s):  
Edward L. Kuff ◽  
George H. Hogeboom ◽  
Albert J. Dalton
Keyword(s):  
Electron Microscopy ◽  
Acid Phosphatase ◽  
Cytochrome C ◽  
Size Distribution ◽  
Phosphatase Activity ◽  
Acid Phosphatase Activity ◽  
Reductase Activity ◽  
Size Group ◽  
Average Radius ◽  
Electron Microscopic

A combined centrifugal, biochemical, and electron microscopic study of the cytoplasmic particulates present in 0.88 M sucrose homogenates of rat liver has been carried out. Size distribution analyses of particles containing pentose nucleic acid (PNA) and exhibiting several types of enzymatic activity revealed three major size groups within the range of particle radius between 10 and 500 mµ. A different array of biochemical properties was associated with each size group. The largest particles, with an average radius (assuming spherical shape) in the region of 220 to 260 mµ, contained all of the succinic dehydrogenase activity of the cytoplasmic extract, 29 per cent of the diphosphopyridine nucleotide (DPN)-cytochrome c reductase activity, and minor amounts of PNA and acid phosphatase activity. Cytologically, this group of particles was identified with the mitochondria. All of the uricase activity, 58 per cent of the acid phosphatase activity, and 26 per cent of the PNA was apparently associated with a second size group of particles (average radius 120 mµ) which were tentatively identified by electron microscopy with vesicular structures derived from the ergastoplasm of the intact cell. The third particle group demonstrated by centrifugation exhibited a major size distribution peak at 25 mµ and a second smaller peak at 55 mµ. Over 50 per cent of the total cytoplasmic PNA and DPN-cytochrome c reductase activity was associated with particles in this size group. Electron microscopy revealed a morphologically heterogeneous population of particles within this size range.

scholarly journals LOCALIZATION OF ACID PHOSPHATASE ACTIVITY IN HEPATIC LYSOSOMES BY MEANS OF ELECTRON MICROSCOPY

1961 ◽  
Vol 9 (4) ◽  
pp. 773-784 ◽  
Author(s):  
E. Essner ◽  
Alex B. Novikoff
Keyword(s):  
Electron Microscopy ◽  
Acid Phosphatase ◽  
Phosphatase Activity ◽  
Acid Phosphatase Activity ◽  
Human Liver ◽  
Osmium Tetroxide ◽  
Frozen Sections ◽  
Fixed Tissue ◽  
Thin Sections ◽  
Lipofuscin Granules

Samples of liver from untreated rats, from rats infused with unconjugated bilirubin, and from biopsies of human liver were fixed overnight in cold formol-calcium. Frozen sections were stained for acid phosphatase activity by the Gomori lead-glycerophosphate procedure. Small blocks of fixed tissue were also incubated in this medium. These were then treated briefly with osmium tetroxide, dehydrated, and embedded in methacrylate. Thin sections were studied by electron microscopy. The sites of reaction product of acid phosphatase activity as visualized in electron micrographs are consistent with those seen in frozen sections studied by light microscopy. They indicate that the pericanalicular bodies of parenchymatous cells, the large spherical bodies of Kupffer cells, the microbodies appearing after bilirubin infusion and lipofuscin granules belong to the class of cytoplasmic organelles called lysosomes by de Duve.

ACID PHOSPHATASE ACTIVITY IN THE PANCREATIC ISLETS OF RATS OF DIFFERENT AGES

1962 ◽  
Vol 40 (4) ◽  
pp. 604-612 ◽  
Author(s):  
Claes Hellerström ◽  
Sven Brolin ◽  
Stig Larsson ◽  
Bo Hellman
Keyword(s):  
Acid Phosphatase ◽  
Pancreatic Islets ◽  
Phosphatase Activity ◽  
Islets Of Langerhans ◽  
Acid Phosphatase Activity ◽  
Early Stage ◽  
Postnatal Life ◽  
Adult Rats ◽  
Different Ages ◽  
Acid Phosphatase Reaction

ABSTRACT Histochemical methods for acid phosphatase and glucose-6-phosphatase were applied to the islets of Langerhans in rats of different ages. It was not possible to demonstrate any specific glucose-6-phosphatase activity, since at the pH optimum of this enzyme, both glucose-6-phosphate and sodium-β-glycerophosphate were hydrolyzed; the more intense reaction occurring with the latter substrate. At the end of the intrauterine life there was a marked acid phosphatase reaction, which appeared unchanged during the first 10 days of postnatal life but was considerably weaker in adult rats. The occurrence of a smaller number of non-reacting cells in the islet periphery was assumed to be evidence that there was no demonstrable acid phosphatase activity in one or both types of A cells. The considerable reaction found in the islets of foetuses and young rats might reflect the maturation and rapid proliferation of these cells during early stage of life.

scholarly journals Fine Structural Demonstration of Acid Phosphatase Activity in Auer Bodies

Blood ◽  
1967 ◽  
Vol 29 (4) ◽  
pp. 667-682 ◽  
Author(s):  
JAMES G. WHITE
Keyword(s):  
Electron Microscopy ◽  
Acid Phosphatase ◽  
Phosphatase Activity ◽  
Acute Myelogenous Leukemia ◽  
Acid Phosphatase Activity ◽  
Myelogenous Leukemia ◽  
Ultrastructural Cytochemistry ◽  
Acute Myelogenous ◽  
Peripheral Leukocytes

Abstract The peripheral leukocytes of three patients with acute myelogenous leukemia have been studied by combined electron microscopy and ultrastructural cytochemistry. Auer bodies present in immature granulocytes were found to contain specific evidence of acid phosphatase activity, indicating that Auer rods are lysosomes. A number of observations were made which support the origin of Auer rod lysosomes from azurophilic granules which are known to be lysosomes. The nature of the stimulus causing transformation or fusion of azure granules to form Auer bodies, however, remains obscure.

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