scholarly journals Fine Structural Demonstration of Acid Phosphatase Activity in Auer Bodies

Blood ◽  
1967 ◽  
Vol 29 (4) ◽  
pp. 667-682 ◽  
Author(s):  
JAMES G. WHITE
Keyword(s):  
Electron Microscopy ◽  
Acid Phosphatase ◽  
Phosphatase Activity ◽  
Acute Myelogenous Leukemia ◽  
Acid Phosphatase Activity ◽  
Myelogenous Leukemia ◽  
Ultrastructural Cytochemistry ◽  
Acute Myelogenous ◽  
Peripheral Leukocytes

Abstract The peripheral leukocytes of three patients with acute myelogenous leukemia have been studied by combined electron microscopy and ultrastructural cytochemistry. Auer bodies present in immature granulocytes were found to contain specific evidence of acid phosphatase activity, indicating that Auer rods are lysosomes. A number of observations were made which support the origin of Auer rod lysosomes from azurophilic granules which are known to be lysosomes. The nature of the stimulus causing transformation or fusion of azure granules to form Auer bodies, however, remains obscure.

Localisation cytochimique des activités phosphatasiques acides de champignons mycorhiziens développés sur milieu complet ou carencé en phosphate

1983 ◽  
Vol 61 (5) ◽  
pp. 1411-1414 ◽  
Author(s):  
Bernadette Lacaze
Keyword(s):  
Electron Microscopy ◽  
Enzyme Activity ◽  
Acid Phosphatase ◽  
Phosphatase Activity ◽  
Cell Walls ◽  
Acid Phosphatase Activity ◽  
Inorganic Phosphate ◽  
Light And Electron Microscopy ◽  
Reaction Products ◽  
Cytochemical Study

The mycelia of three mycorrhizal basidiomycètes (Pisolithus tinctorius (Pers.) Coker et Couch., Suillus granulatus (L. ex Fr.) O. Kuntze and S. bellinii (Izenga) Watling) were grown on media with or without inorganic phosphate. A cytochemical study of the distribution of acid phosphatase activity was made using light and electron microscopy. Highly enhanced enzyme activity was observed in the phosphorus-deficient mycelia. Precipitates were located primarily at the surface of the fungal cells. Cell walls appear devoid of reaction products in most cases.

scholarly journals CENTRIFUGAL, BIOCHEMICAL, AND ELECTRON MICROSCOPIC ANALYSIS OF CYTOPLASMIC PARTICULATES IN LIVER HOMOGENATES

1956 ◽  
Vol 2 (1) ◽  
pp. 33-54 ◽  
Author(s):  
Edward L. Kuff ◽  
George H. Hogeboom ◽  
Albert J. Dalton
Keyword(s):  
Electron Microscopy ◽  
Acid Phosphatase ◽  
Cytochrome C ◽  
Size Distribution ◽  
Phosphatase Activity ◽  
Acid Phosphatase Activity ◽  
Reductase Activity ◽  
Size Group ◽  
Average Radius ◽  
Electron Microscopic

A combined centrifugal, biochemical, and electron microscopic study of the cytoplasmic particulates present in 0.88 M sucrose homogenates of rat liver has been carried out. Size distribution analyses of particles containing pentose nucleic acid (PNA) and exhibiting several types of enzymatic activity revealed three major size groups within the range of particle radius between 10 and 500 mµ. A different array of biochemical properties was associated with each size group. The largest particles, with an average radius (assuming spherical shape) in the region of 220 to 260 mµ, contained all of the succinic dehydrogenase activity of the cytoplasmic extract, 29 per cent of the diphosphopyridine nucleotide (DPN)-cytochrome c reductase activity, and minor amounts of PNA and acid phosphatase activity. Cytologically, this group of particles was identified with the mitochondria. All of the uricase activity, 58 per cent of the acid phosphatase activity, and 26 per cent of the PNA was apparently associated with a second size group of particles (average radius 120 mµ) which were tentatively identified by electron microscopy with vesicular structures derived from the ergastoplasm of the intact cell. The third particle group demonstrated by centrifugation exhibited a major size distribution peak at 25 mµ and a second smaller peak at 55 mµ. Over 50 per cent of the total cytoplasmic PNA and DPN-cytochrome c reductase activity was associated with particles in this size group. Electron microscopy revealed a morphologically heterogeneous population of particles within this size range.

scholarly journals The Localization of Acid Phosphatase in the Sieve Element of Pisum

1969 ◽  
Vol 22 (4) ◽  
pp. 1051 ◽  
Author(s):  
S-Y Zee
Keyword(s):  
Electron Microscopy ◽  
Acid Phosphatase ◽  
Light Microscopy ◽  
Phosphatase Activity ◽  
Acid Phosphatase Activity ◽  
Lead Nitrate ◽  
Sieve Tube ◽  
Sieve Element ◽  
Recent Advances ◽  
Acid Phosphatase Reaction

Acid phosphatase activity has been detected within the sieve-tube members of plants by many workers using the Gomori technique and light microscopy (Lester and Evert 1965; Flinn and Smith 1967). Unfortunately the limited resolution makes it difficult to determine the specific sites of activity of the reaction product of the enzyme; recent advances in histochemical techniques for electron microscopy have made it possible to investigate more specifically the sites of localization of the acid phosphatase reaction product by using the Gomori lead nitrate technique (Goldfischer, Essner, and Novikoff 1964; Catesson and Czanenski 1967; Bowen 1968; Figier 1968; Wardrop 1968).

scholarly journals LOCALIZATION OF ACID PHOSPHATASE ACTIVITY IN HEPATIC LYSOSOMES BY MEANS OF ELECTRON MICROSCOPY

1961 ◽  
Vol 9 (4) ◽  
pp. 773-784 ◽  
Author(s):  
E. Essner ◽  
Alex B. Novikoff
Keyword(s):  
Electron Microscopy ◽  
Acid Phosphatase ◽  
Phosphatase Activity ◽  
Acid Phosphatase Activity ◽  
Human Liver ◽  
Osmium Tetroxide ◽  
Frozen Sections ◽  
Fixed Tissue ◽  
Thin Sections ◽  
Lipofuscin Granules

Samples of liver from untreated rats, from rats infused with unconjugated bilirubin, and from biopsies of human liver were fixed overnight in cold formol-calcium. Frozen sections were stained for acid phosphatase activity by the Gomori lead-glycerophosphate procedure. Small blocks of fixed tissue were also incubated in this medium. These were then treated briefly with osmium tetroxide, dehydrated, and embedded in methacrylate. Thin sections were studied by electron microscopy. The sites of reaction product of acid phosphatase activity as visualized in electron micrographs are consistent with those seen in frozen sections studied by light microscopy. They indicate that the pericanalicular bodies of parenchymatous cells, the large spherical bodies of Kupffer cells, the microbodies appearing after bilirubin infusion and lipofuscin granules belong to the class of cytoplasmic organelles called lysosomes by de Duve.

scholarly journals Colony Formation by Normal and Leukemic Human Marrow Cells in Culture: Effect of Conditioned Medium From Human Leukocytes

Blood ◽  
1971 ◽  
Vol 37 (1) ◽  
pp. 1-5 ◽  
Author(s):  
N. N. ISCOVE ◽  
J. S. SENN ◽  
J. E. TILL ◽  
E. A. MCCULLOCH
Keyword(s):  
Cell Culture ◽  
Conditioned Medium ◽  
Acute Myelogenous Leukemia ◽  
Colony Formation ◽  
Myelogenous Leukemia ◽  
Human Leukocytes ◽  
Cells In Culture ◽  
Acute Myelogenous ◽  
Peripheral Leukocytes ◽  
Marrow Cells

Abstract "Conditioned medium" obtained from cultures of human peripheral leukocytes promoted the growth of human marrow cells in cell culture. This material also permitted the growth of small colonies from the marrow of patients with acute myelogenous leukemia in relapse; in its absence, only occasional colonies were observed.

Erythrophagocytosis in the Liver in Hemolytic Anemias

1968 ◽  
Vol 26 ◽  
pp. 184-185
Author(s):  
O. T. Minick ◽  
E. Orfei ◽  
F. Volini ◽  
G. Kent
Keyword(s):  
Acid Phosphatase ◽  
Oxidative Damage ◽  
Phosphatase Activity ◽  
Kupffer Cells ◽  
Acid Phosphatase Activity ◽  
Common Type ◽  
Red Cell ◽  
Cell Fragments ◽  
Reticulo Endothelial System ◽  
Important Site

Hemolytic anemias were produced in rats by administering phenylhydrazine or anti-erythrocytic (rooster) serum, the latter having agglutinin and hemolysin titers exceeding 1:1000.Following administration of phenylhydrazine, the erythrocytes undergo oxidative damage and are removed from the circulation by the cells of the reticulo-endothelial system, predominantly by the spleen. With increasing dosage or if animals are splenectomized, the Kupffer cells become an important site of sequestration and are greatly hypertrophied. Whole red cells are the most common type engulfed; they are broken down in digestive vacuoles, as shown by the presence of acid phosphatase activity (Fig. 1). Heinz body material and membranes persist longer than native hemoglobin. With larger doses of phenylhydrazine, erythrocytes undergo intravascular fragmentation, and the particles phagocytized are now mainly red cell fragments of varying sizes (Fig. 2).

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