Preparation and Biocompatibility Characterization of Regenerated Silk Fibroin Films

2021 ◽  
pp. 1-13
Author(s):  
Xuan Zhang ◽  
Dong Zhu ◽  
Yuan Cheng ◽  
Xiaohan Zhang ◽  
Xiaolan Guo ◽  
...  
Keyword(s):  
Silk Fibroin ◽  
Regenerated Silk Fibroin

scholarly journals Fractionation of Regenerated Silk Fibroin and Characterization of the Fractions

Molecules ◽  
2021 ◽  
Vol 26 (20) ◽  
pp. 6317
Author(s):  
Masaaki Aoki ◽  
Yu Masuda ◽  
Kota Ishikawa ◽  
Yasushi Tamada
Keyword(s):  
Mechanical Properties ◽  
Secondary Structure ◽  
Silk Fibroin ◽  
Gel Permeation Chromatography ◽  
Precipitation Process ◽  
Regenerated Silk Fibroin ◽  
Sds Page ◽  
Fractionation Method ◽  
Nonwoven Mats

The molecular weight (MW) of regenerated silk fibroin (RSF) decreases during degumming and dissolving processes. Although MW and the MW distribution generally affect polymer material processability and properties, few reports have described studies examining the influences of MW and the distribution on silk fibroin (SF) material. To prepare different MW SF fractions, the appropriate conditions for fractionation of RSF by ammonium sulfate (AS) precipitation process were investigated. The MW and the distribution of each fraction were found using gel permeation chromatography (GPC) and SDS-polyacrylamide electrophoresis (SDS-PAGE). After films of the fractionated SFs formed, the secondary structure, surface properties, and cell proliferation of films were evaluated. Nanofiber nonwoven mats and 3D porous sponges were fabricated using the fractionated SF aqueous solution. Then, their structures and mechanical properties were analyzed. The results showed AS precipitation using a dialysis membrane at low temperature to be a suitable fractionation method for RSF. Moreover, MW affects the nanofiber and sponge morphology and mechanical properties, although no influence of MW was observed on the secondary structure or crystallinity of the fabricated materials.

scholarly journals Insights into the production and characterization of electrospun fibers from regenerated silk fibroin

2014 ◽  
Vol 60 ◽  
pp. 123-134 ◽  
Author(s):  
Concepción Solanas ◽  
Sara Herrero ◽  
Aravind Dasari ◽  
Gustavo R. Plaza ◽  
Javier Llorca ◽  
...  
Keyword(s):  
Silk Fibroin ◽  
Electrospun Fibers ◽  
Regenerated Silk Fibroin

Extraction and Characterization of Silk Fibroin from Waste Silk

2013 ◽  
Vol 788 ◽  
pp. 174-177 ◽  
Author(s):  
Hao Liu ◽  
Ju Wei ◽  
Lai Jiu Zheng ◽  
Yu Ping Zhao
Keyword(s):  
Aqueous Solution ◽  
Weight Loss ◽  
Glass Transition ◽  
Calcium Chloride ◽  
Silk Fibroin ◽  
Chloride Concentration ◽  
Silk Fibers ◽  
Regenerated Silk Fibroin ◽  
Dialysis Method

Waste silk fibers were dissolved in calcium chloride aqueous solution. Effect of calcium chloride concentration on silk solubility was studied. Dialysis method was used to purify the dissolved silk fibroin. The purified silk fibroin was characterized by FTIR and thermoanalysis technique. Results showed that the purified silk fibroin mainly had irregular curly conformation. The glass transition temperature of the regenerated silk fibroin reached 90.6°C-135°C, higher than 78.9°C-135°C before purification. Thermal weight loss temperature by 50% was 490.3°C differing from 429.7°C before purification.

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