In vivo evaluation of bilayer ORC/PCL composites in a rabbit model for using as a dural substitute

2020 ◽  
Vol 42 (10) ◽  
pp. 879-889
Author(s):  
Sorayouth Chumnanvej ◽  
Ticomporn Luangwattanawilai ◽  
Visut Rawiwet ◽  
Jintamai Suwanprateeb ◽  
Kasem Rattanapinyopituk ◽  
...  
Keyword(s):  
Rabbit Model ◽  
In Vivo Evaluation ◽  
Dural Substitute

scholarly journals Development of Triamcinolone Acetonide-Loaded Microemulsion as a Prospective Ophthalmic Delivery System for Treatment of Uveitis: In Vitro and In Vivo Evaluation

Pharmaceutics ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 444
Author(s):  
Alaa Mahran ◽  
Sayed Ismail ◽  
Ayat A. Allam
Keyword(s):  
Triamcinolone Acetonide ◽  
Delivery System ◽  
Histopathological Examination ◽  
Rabbit Model ◽  
Subconjunctival Injection ◽  
In Vivo Evaluation ◽  
Ternary Phase Diagrams ◽  
Ophthalmic Delivery

Treatment of uveitis (i.e., inflammation of the uvea) is challenging due to lack of convenient ophthalmic dosage forms. This work is aimed to determine the efficiency of triamcinolone acetonide (TA)-loaded microemulsion as an ophthalmic delivery system for the treatment of uveitis. Water titration method was used to construct different pseudo-ternary phase diagrams. Twelve microemulsion formulations were prepared using oleic acid, Cremophor EL, and propylene glycol. Among all tested formulations, Formulation F3, composed of oil: surfactant-co-surfactant (1:1): water (15:35:50% w/w, respectively), was found to be stable and showed acceptable pH, viscosity, conductivity, droplet size (211 ± 1.4 nm), and zeta potential (−25 ± 1.7 mV) and almost complete in vitro drug release within 24 h. The in vivo performance of the optimized formulation was evaluated in experimentally uveitis-induced rabbit model and compared with a commercial TA suspension (i.e., Kenacort®-A) either topically or by subconjunctival injection. Ocular inflammation was evaluated by clinical examination, white blood cell count, protein content measurement, and histopathological examination. The developed TA-loaded microemulsion showed superior therapeutic efficiency in the treatment of uveitis with high patient compliance compared to commercial suspension. Hence, it could be considered as a potential ocular treatment option in controlling of uveitis.

In vivo evaluation of a new hydrophobic acrylic intraocular lens in the rabbit model

2018 ◽  
Vol 44 (12) ◽  
pp. 1497-1502 ◽  
Author(s):  
Liliana Werner ◽  
Nathan Ellis ◽  
Joshua Bo Heczko ◽  
Marcia Ong ◽  
Rakhi Jain ◽  
...  
Keyword(s):  
Intraocular Lens ◽  
Rabbit Model ◽  
In Vivo Evaluation ◽  
Hydrophobic Acrylic

In Vivo Evaluation of The Novel Nanocomposite Porous 3D Scaffold in a Rabbit Model

2018 ◽  
Vol 11 (19) ◽  
pp. 1-15
Author(s):  
Saffanah Khuder Mahmood ◽  
Intan Shameha Binti Abdul Razak ◽  
Sahar Mohammed Ibrahim ◽  
Loqman Mohamed Yusof ◽  
Adamu Abdul Abubakar ◽  
...  
Keyword(s):  
Rabbit Model ◽  
The Novel ◽  
3D Scaffold ◽  
In Vivo Evaluation

scholarly journals In Vivo Evaluation of the Mechanical Strength of a Slide Lengthening Technique With a Locking Mechanism Using a Rabbit Model

Cureus ◽  
2020 ◽  
Author(s):  
Dai Iwase ◽  
Kentaro Uchida ◽  
Yukie Metoki ◽  
Hiroyuki Sekiguchi ◽  
Jun Aikawa ◽  
...  
Keyword(s):  
Mechanical Strength ◽  
Rabbit Model ◽  
In Vivo Evaluation ◽  
Locking Mechanism

Bone regeneration strategy by different sized multichanneled biphasic calcium phosphate granules: In vivo evaluation in rabbit model

2018 ◽  
Vol 32 (10) ◽  
pp. 1406-1420 ◽  
Author(s):  
Mirana Taz ◽  
Sang Ho Bae ◽  
Hae Il Jung ◽  
Hyun-Deuk Cho ◽  
Byong-Taek Lee
Keyword(s):  
Calcium Phosphate ◽  
Bone Formation ◽  
Rabbit Model ◽  
Morphological Characteristics ◽  
Confocal Imaging ◽  
Bone Tissue Regeneration ◽  
Micro Computed Tomography ◽  
In Vivo Evaluation

A variety of synthetic materials are currently in use as bone substitutes, among them a new calcium phosphate-based multichannel, cylindrical, granular bone substitute that is showing satisfactory biocompatibility and osteoconductivity in clinical applications. These cylindrical granules differ in their mechanical and morphological characteristics such as size, diameter, surface area, pore size, and porosity. The aim of this study is to investigate whether the sizes of these synthetic granules and the resultant inter-granular spaces formed by their filling critical-sized bone defects affect new bone formation characteristics and to determine the best formulations from these individual types by combining the granules in different proportions to optimize the bone tissue regeneration. We evaluated two types of multichanneled cylindrical granules, 1 mm and 3 mm in diameter, combined the granules in two different proportions (wt%), and compared their different mechanical, morphological, and in vitro and in vivo biocompatibility characteristics. We assessed in vitro biocompatibility and cytotoxicity using MC3T3-E1 osteoblast-like cells using MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay and confocal imaging. In vivo investigation in a rabbit model indicated that all four samples formed significantly better bone than the control after four weeks and eight weeks of implantation. Micro-computed tomography analysis showed more bone formation by the 1 mm cylindrical granules with 160 ± 10 µm channeled pore and 50% porosity than the other three samples ( p<.05), which we confirmed by histological analysis.

scholarly journals In vitro and in vivo evaluation of a dextran-graft-polybutylmethacrylate copolymer coated on CoCr metallic stent

Bioimpacts ◽  
2018 ◽  
Vol 9 (1) ◽  
pp. 25-36 ◽  
Author(s):  
Cécilia Delattre ◽  
Diego Velazquez ◽  
Caroline Roques ◽  
Graciela Pavon-Djavid ◽  
Véronique Ollivier ◽  
...  
Keyword(s):  
Bacterial Adhesion ◽  
Neointimal Hyperplasia ◽  
Rabbit Model ◽  
Metallic Stent ◽  
In Vivo Evaluation ◽  
Endothelial Colony Forming Cells ◽  
Cell Coverage ◽  
Stent Coating

Introduction: The major complications of stent implantation are restenosis and late stent thrombosis. PBMA polymers are used for stent coating because of their mechanical properties. We previously synthesized and characterized Dextrangraft-polybutylmethacrylate copolymer (Dex-PBMA) as a potential stent coating. In this study, we evaluated the haemocompatibility and biocompatibility properties of Dex-PBMA in vitro and in vivo. Methods: Here, we investigated: (1) the effectiveness of polymer coating under physiological conditions and its ability to release Tacrolimus®, (2) the capacity of Dex-PBMA to inhibit Staphylococcus aureus adhesion, (3) the thrombin generation and the human platelet adhesion in static and dynamic conditions, (4) the biocompatibility properties in vitro on human endothelial colony forming cells ( ECFC) and on mesenchymal stem cells (MSC) and in vivo in rat models, and (5) we implanted Dex-PBMA and Dex-PBMATAC coated stents in neointimal hyperplasia restenosis rabbit model. Results: Dex-PBMA coating efficiently prevented bacterial adhesion and release Tacrolimus®. Dex-PBMA exhibit haemocompatibility properties under flow and ECFC and MSC compatibility. In vivo, no pathological foreign body reaction was observed neither after intramuscular nor intravascular aortic implantation. After Dex-PBMA and Dex-PBMATAC coated stents 30 days implantation in a restenosis rabbit model, an endothelial cell coverage was observed and the lumen patency was preserved. Conclusion: Based on our findings, Dex-PBMA exhibited vascular compatibility and can potentially be used as a coating for metallic coronary stents.

scholarly journals In vivo Evaluation of Fibrous Collagen Dura Substitutes

2021 ◽  
Vol 9 ◽  
Author(s):  
Wenbo Liu ◽  
Xin Wang ◽  
Jinlei Su ◽  
Qingsong Jiang ◽  
Jing Wang ◽  
...  
Keyword(s):  
Type I Collagen ◽  
Rabbit Model ◽  
Surrounding Tissue ◽  
Control Group ◽  
Regeneration Ability ◽  
Type I ◽  
Blank Control Group ◽  
In Vivo Evaluation ◽  
And Performance

Dura substitutes are applied in duraplasty to repair lost or damaged dura. Collagen-based dura substitutes are mainstream products in both the US and Chinese markets. In this study, dura substitute devices with potential dura regeneration ability are evaluated. The dura substitutes are composed of fibrous type I collagen that were purified from bovine tendon. Physical and chemical characterization demonstrated that the tested dura substitute has desirable porous scaffolding structures and is composed of highly purified type I collagen. The collagen dura substitutes were further investigated in vivo with a rabbit model for 6 months to evaluate their safety and performance to repair and regenerate dura. No inflammation or infection was observed during the course of in vivo study. The integration of the collagen dura substitutes with surrounding tissue was normal as compared to native tissue. The macroscopic and microscopic histological assessments of the sampled animal tissue showed that the damaged dura were regenerated. The collagen dura substitutes were resorbed between 3 and 6 months along with newly regenerated dura. Both tissue adhesion and dura repair was the worst in blank control group as compared to those in the collagen dura substitutes. Taken together, regenerative collagen dura substitutes demonstrated with suitable physicochemical properties. The in vivo evaluation in a rabbit model further demonstrated the safety and performance of such substitutes for dura repair and regeneration.

scholarly journals In vivo evaluation of a novel nanocomposite porous 3D scaffold in a rabbit model: histological analysis

2017 ◽  
Vol Volume 12 ◽  
pp. 8587-8598 ◽  
Author(s):  
Saffanah Mahmood ◽  
Abdul Razak Intan-Shameha ◽  
Mustafa Ghaji ◽  
Loqman Mohamad Yusof ◽  
Zaid Mahmood ◽  
...  
Keyword(s):  
Histological Analysis ◽  
Rabbit Model ◽  
3D Scaffold ◽  
In Vivo Evaluation

In vitro and in vivo evaluation of sunitinib eluting beads.

2013 ◽  
Vol 31 (4_suppl) ◽  
pp. 246-246
Author(s):  
Pierre E. Bize ◽  
Olivier Jordan ◽  
Katrin Fuchs ◽  
Olivier Dormond ◽  
Rafael Duran ◽  
...  
Keyword(s):  
Hepatic Artery ◽  
Drug Loading ◽  
Rabbit Model ◽  
Internal Standard ◽  
Embolic Agent ◽  
Liver Malignancies ◽  
In Vivo Evaluation ◽  
High Drug

246 Background: Chemoembolization is used to treat liver malignancies. However recurrence occurs frequently, possibly because of neoangiogenesis triggered by ischemia caused by the embolic agent. In this context, the combination of an embolic agent with an anti-angiogenic drug seems appealing. This study characterizes the in vitro loading and release profile of sunitinib eluting beads of different sizes and their pharmacokinetic profile in a rabbit model. Methods: 70-150 μm and 100-300 μm drug eluting beads (DC Bead, Biocompatibles UK) were loaded by incubation in a sunitinib hydrochloride solution. Drug was quantified by spectrophotometry at 430 nm. Drug release was measured over one-week periods and normalized using an internal standard in 30% ethanol in NaCl 0.9%. New-Zealand white rabbits were used. Eight animals received 0.2 ml of 100-300 μm DC Bead loaded with 6 mg of sunitinib in the hepatic artery (group 1) and 4 animals received 6 mg of sunitinib p.o. (group 2). Half of the animals were sacrificed after 6 hours and half after24 hours. Liver enzymes were measured at 0, 6 and 24 hours in both groups. Plasmatic sunitinib concentration was determined by tandem mass spectroscopy (LC MS/MS) at 0, 1, 2, 3, 4, 5, 6 and 24 hours. At sacrifice, the livers were harvested and sunitinib concentration in liver tissue was assessed by LC MS/MS. Results: High drug loading was obtained for both microsphere bead sizes. Particle shrinking was observed with adsorption of sunitinib. Almost complete release of sunitinib was detected under physiological conditions, with very similar release for 70-150 μm and 100-300 μm (t50%=1.2 h) DC Bead. Conclusions: Sunitinib eluting beads are well tolerated by rabbits when administered in the hepatic artery. No unexpected toxicity was observed. Very high drug concentration can be obtained at the site of embolization with minimal systemic passage.

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