Significance of long non-coding RNA IFNG-AS1 in the progression and clinical prognosis in colon adenocarcinoma

Abstract Background We performed this meta-analysis to elucidate whether the expression of PlncRNA-1 might serve as an effective prognostic marker for various cancers. Methods We conducted a database search of PubMed, ScienceDirect, Embase, Web of Science and CNKI database (up to Oct 31, 2019). The pooled hazard ratio (HR), odds ratio (OR) and 95% confidence interval (CI) were used to estimate the strength of the relationship between PlncRNA-1 expression and the clinical prognosis of cancer patients. Results The results showed that elevated PlncRNA-1 expression predicted a poor OS with pooled HRs of 1.43 (95% CI: 1.25-1.63, I 2 =63.1%, P=0.004). Likewise, we found that advanced tumour stages were associated with upregulated PlncRNA-1 expression in various cancer types (III–IV vs I–II: OR=2.79, 95% CI: 1.76-4.41, I 2 =0%, P=0.822),patients with high PlncRNA-1 expression might have an increased risk of large tumours (OR=2.03, 95% CI: 1.31-3.14, I 2 =67.1%, P=0.028). Conclusions PlncRNA-1 might be used as a prognostic biomarker and as a tool for the early detection of various tumours.

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Integration analysis of long non-coding RNA (lncRNA) role in tumorigenesis of colon adenocarcinoma

BMC Medical Genomics ◽

10.1186/s12920-020-00757-2 ◽

2020 ◽

Vol 13 (1) ◽

Author(s):

Arash Poursheikhani ◽

Mohammad Reza Abbaszadegan ◽

Negin Nokhandani ◽

Mohammad Amin Kerachian

Keyword(s):

Colon Adenocarcinoma ◽

Non Coding Rna ◽

Long Non Coding Rna ◽

Integration Analysis

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LINC01123 facilitates proliferation, invasion and chemoresistance of colon cancer cells

Bioscience Reports ◽

10.1042/bsr20194062 ◽

2020 ◽

Vol 40 (8) ◽

Author(s):

Shicai Ye ◽

Bilan Sun ◽

Weiyun Wu ◽

Caiyuan Yu ◽

Ting Tian ◽

...

Keyword(s):

Colon Cancer ◽

Cancer Cells ◽

Cancer Progression ◽

Colon Adenocarcinoma ◽

Colon Cancer Cells ◽

Prognostic Signature ◽

Small Cell Lung ◽

Non Coding Rna ◽

Colon Cancer Progression ◽

Long Non Coding Rna

Abstract Colon cancer is one of the major causes of cancer-related deaths worldwide. Long non-coding RNA (lncRNA) LINC01123 has been suggested to act as an oncogene in non-small cell lung cancer and a prognostic signature in head and neck squamous cell carcinoma. However, its role in colon cancer remains obscure. From TCGA database, LINC01123 was observed to be up-regulated in colon adenocarcinoma (COAD). Subsequently, the up-regulated LINC01123 was also detected in colon cancer cells. Functionally, LINC01123 could enhance cell proliferation, migration, invasion and angiogenesis. Moreover, the chemoresistance of colon cancer cells was verified to be promoted by LINC01123. Afterward, LINC01123 was found to bind with Ago2 and miR-34c-5p. Besides, miR-34c-5p was confirmed to inhibit the cellular process and chemoresistance of colon cancer cells. Then, VEGFA was disclosed to coexist with LINC01123 and miR-34c-5p in RNA-induced silencing complex. And TCGA database suggested that its expression was correlated with different stages of COAD. Moreover, it was uncovered that VEGFA could bind with miR-34c-5p and its expression positively correlated with LINC01123 expression. Finally, LINC01123 was proofed to regulate colon cancer progression and cells chemoresistance via VEGFA. In conclusion, LINC01123/miR-34c-5p/VEGFA axis promotes colon cancer malignancy and cells chemoresistance.

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Long non‐coding RNA ZEB1‐AS1 promotes colon adenocarcinoma malignant progression via miR‐455‐3p/PAK2 axis

Cell Proliferation ◽

10.1111/cpr.12723 ◽

2019 ◽

Vol 53 (1) ◽

Cited By ~ 1

Author(s):

Xin Ni ◽

Yuting Ding ◽

Haitao Yuan ◽

Jinmin Shao ◽

Yan Yan ◽

...

Keyword(s):

Colon Adenocarcinoma ◽

Malignant Progression ◽

Non Coding Rna ◽

Long Non Coding Rna

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Abstract 1817: Differential expression of long non-coding RNA in colon adenocarcinoma RNA-sequence data set

10.1158/1538-7445.sabcs18-1817 ◽

2019 ◽

Author(s):

Stephen J. O'Brien ◽

Theodore Kalbfleisch ◽

Sudhir Srivastava ◽

Shesh Rai ◽

Susan Galandiuk

Keyword(s):

Differential Expression ◽

Sequence Data ◽

Colon Adenocarcinoma ◽

Data Set ◽

Rna Sequence ◽

Non Coding Rna ◽

Long Non Coding Rna

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LncRNA ACTA2-AS1 suppress colon adenocarcinoma progression by sponging miR-4428 upregulation BCL2L11

Cancer Cell International ◽

10.1186/s12935-021-01769-3 ◽

2021 ◽

Vol 21 (1) ◽

Author(s):

Qingyun Pan ◽

Ying Huang ◽

Yirui Wang ◽

Deke Li ◽

Changjiang Lei

Keyword(s):

Cell Proliferation ◽

Colony Formation ◽

Colon Adenocarcinoma ◽

Luciferase Reporter Assay ◽

Luciferase Reporter ◽

Reporter Assay ◽

Over Expression ◽

Non Coding Rna ◽

Long Non Coding Rna

Abstract Background Long non-coding RNA is considered to be essential to modulate the development and progression of human malignant cancers. And long non-coding RNA can act as crucial modulators by sponging the corresponding microRNA in tumorigenesis. We aimed to elucidate the function of ACTA2-AS1 and its molecular mechanism in colon adenocarcinoma. Materials and methods The expression of ACTA2-AS1, miR-4428 and BCL2L11 in colon adenocarcinoma tissues were detected via qRT-PCR. SW480 and HT29 cells were transfected with shRNA ACTA2-AS1, OE ACTA2-AS1, miRNA mimics of miR-4428, miR-4428 inhibitor, si-BCL2L11 and over-expression of si-BCL2L11. Cell proliferation, colony formation and apoptosis were respectively assessed using CCK-8 assay, colony assay and flow cytometry. Luciferase reporter assay was performed to verify the targets of ACTA2-AS1 and miR-4428. Tumor subcutaneous xenograft mode was constructed to explore tumor growth in vivo. Results ACTA2-AS1 was obviously downregulated in human colon adenocarcinoma tissues and colon adenocarcinoma cell lines. Silence or over-expression of ACTA2-AS1 promoted or inhibited cell proliferation and colony formation abilities, and regulated apoptosis. The silence of ACTA2-AS1 resulted in the decrease of Bax and increase of Bal2, while restored in OE ACTA2-AS1 group when compared with the control transfected cells. In addition, luciferase reporter assay revealed that ACTA2-AS1 interacted with miR-4428 and suppressed its expression. miR-4428 could bind to 3ʹ untranslated region of BCL2L11 and modulated the expression of BCL2L11 negatively. Knockdown of ACTA2-AS1 and over-expression of BCL2L11 reversed the biological function that ACTA2-AS1 mediated by knockdown ACTA2-AS1 alone. Conclusion Our data demonstrated that ACTA2-AS1 could suppress colon adenocarcinoma progression via sponging miR-4428 to regulate BCL2L11 expression.

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Long non-coding RNA VPS9D1-AS1 promotes growth of colon adenocarcinoma by sponging miR-1301-3p and CLDN1

Human Cell ◽

10.1007/s13577-021-00604-1 ◽

2021 ◽

Author(s):

Wei Liu

Keyword(s):

Colon Adenocarcinoma ◽

Non Coding Rna ◽

Long Non Coding Rna

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Long non-coding RNA ZFAS1 is a major regulator of epithelial-mesenchymal transition through miR-200/ZEB1/E-cadherin, vimentin signaling in colon adenocarcinoma

Cell Death Discovery ◽

10.1038/s41420-021-00427-x ◽

2021 ◽

Vol 7 (1) ◽

Author(s):

Stephen J. O’Brien ◽

Casey Fiechter ◽

James Burton ◽

Jacob Hallion ◽

Mason Paas ◽

...

Keyword(s):

Cellular Proliferation ◽

Epithelial Mesenchymal Transition ◽

Colon Adenocarcinoma ◽

Signaling Cascade ◽

Migration And Invasion ◽

Normal Colon ◽

Mesenchymal Transition ◽

Non Coding Rna ◽

Long Non Coding Rna ◽

E Cadherin

AbstractColon adenocarcinoma is a common cause of cancer-related deaths worldwide. Epithelial-mesenchymal transition is a major regulator of cancer metastasis, and increased understanding of this process is essential to improve patient outcomes. Long non-coding RNA (lncRNA) are important regulators of carcinogenesis. To identify lncRNAs associated with colon carcinogenesis, we performed an exploratory differential gene expression analysis comparing paired colon adenocarcinoma and normal colon epithelium using an RNA-sequencing data set. This analysis identified lncRNA ZFAS1 as significantly increased in colon cancer compared to normal colon epithelium. This finding was validated in an institutional cohort using laser capture microdissection. ZFAS1 was also found to be principally located in the cellular cytoplasm. ZFAS1 knockdown was associated with decreased cellular proliferation, migration, and invasion in two colon cancer cell lines (HT29 and SW480). MicroRNA-200b and microRNA-200c (miR-200b and miR-200c) are experimentally validated targets of ZFAS1, and this interaction was confirmed using reciprocal gene knockdown. ZFAS1 knockdown regulated ZEB1 gene expression and downstream targets E-cadherin and vimentin. Knockdown of miR-200b or miR-200c reversed the effect of ZFAS1 knockdown in the ZEB1/E-cadherin, vimentin signaling cascade, and the effects of cellular migration and invasion, but not cellular proliferation. ZFAS1 knockdown was also associated with decreased tumor growth in an in vivo mouse model. These results demonstrate the critical importance of ZFAS1 as a regulator of the miR-200/ZEB1/E-cadherin, vimentin signaling cascade.

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