scholarly journals A new screening system for entry inhibitors based on cell-to-cell transmitted syncytia formation mediated by self-propagating hybrid VEEV-SARS-CoV-2 replicon

2022 ◽  
pp. 1-28
Author(s):  
Na Li ◽  
Xiao-Ling Chen ◽  
Qi Li ◽  
Zhe-Rui Zhang ◽  
Cheng-Lin Deng ◽  
...  
Keyword(s):  
Screening System ◽  
Entry Inhibitors ◽  
Syncytia Formation

Development and Evaluation of a FACS-Based Medium-Throughput Assay for HCV Entry Inhibitors

2009 ◽  
Vol 14 (6) ◽  
pp. 620-626 ◽  
Author(s):  
Sigrid Ziegler ◽  
Bernd Kronenberger ◽  
Beatrice A.-M. Albrecht ◽  
Artur Kaul ◽  
Anna-Lena Gamer ◽  
...  
Keyword(s):  
Envelope Glycoprotein ◽  
Basic Research ◽  
Cell Entry ◽  
Luciferase Reporter ◽  
Human Hepatoma ◽  
Screening System ◽  
Human Hepatoma Cells ◽  
Entry Inhibitors ◽  
Fast Screening ◽  
Key Events

The interaction between the hepatitis C virus (HCV) envelope glycoprotein E2 and the human tetraspanin protein CD81 is one of the key events involved in HCV cell entry. Therefore, compounds that interfere with this interaction may be useful tools for basic research and potential drugs for the treatment of HCV infection. The authors describe a medium-throughput assay for ligands of the E2 binding site on the CD81 receptor. In the assay, human hepatoma cells are incubated with the test compounds and stained with a fluorescently labeled anti-CD81 antibody (JS81). Flow cytometry is used to detect the level of bound antibody, reflecting the inhibitory potencies of the compounds. Eighty percent of compounds active in the assay show efficacy in an infection assay using luciferase reporter genome in cell culture. Thus, the assay can be used as a fast screening system for inhibitors of interaction of viral E2 to host cell CD81-LELs. ( Journal of Biomolecular Screening 2009;620-626)

Mental health screening system using intranet setting in a company.

1999 ◽  
Author(s):  
Minoru Arai ◽  
Daisuke Mori ◽  
Tetsu Kawamura ◽  
Hideo Fumimoto ◽  
Masagi Shimazaki ◽  
...  
Keyword(s):  
Mental Health ◽  
Health Screening ◽  
Mental Health Screening ◽  
Screening System ◽  
A Company

Glucagon induziert eine signifikante nukleäre Translokation von FOXO1, nachgewiesen mit einem neu entwickelten, hochsensitiven, GFP-FOXO1 basierten screening System

2010 ◽  
Vol 5 (S 01) ◽  
Author(s):  
C Bumke-Vogt ◽  
A Arafat ◽  
M Osterhoff ◽  
H Elkatry ◽  
A Ziegenhorn ◽  
...  
Keyword(s):  
Screening System

The Design Concept of the Saving Energy for OCC Screening System

2005 ◽  
Vol 59 (6) ◽  
pp. 819-824
Author(s):  
Toshio Dousaka ◽  
Naoyuki Iwashige
Keyword(s):  
Design Concept ◽  
Screening System ◽  
Saving Energy

A Web-Based Screening System For Dyslexic Pupils: Do Teachers Need It?

2012 ◽  
Vol 5 (4) ◽  
pp. 15-23
Author(s):  
Nor Hasbiah Bt. Ubaidullah ◽  
◽  
Jamilah Hamid ◽  
Keyword(s):  
Screening System ◽  
Web Based

3D-QSAR Methodologies and Molecular Modeling in Bioinformatics for the Search of Novel Anti-HIV Therapies: Rational Design of Entry Inhibitors

2013 ◽  
Vol 8 (4) ◽  
pp. 452-464 ◽  
Author(s):  
Alejandro Speck-Planche ◽  
Valeria Kleandrova ◽  
Marcus Scotti ◽  
M. Cordeiro
Keyword(s):  
Molecular Modeling ◽  
Rational Design ◽  
3D Qsar ◽  
Entry Inhibitors ◽  
Anti Hiv
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