scholarly journals Callus induction and flavonoid production on the immature seed of Stelechocarpus burahol

2018 ◽  
Vol 983 ◽  
pp. 012186 ◽  
Author(s):  
N A Habibah ◽  
S Moeljopawiro ◽  
K Dewi ◽  
A Indrianto
1994 ◽  
Vol 90 (4) ◽  
pp. 739-747 ◽  
Author(s):  
Diana Lee ◽  
Barbara A. Moffatt

2009 ◽  
Vol 24 ◽  
pp. 82-88 ◽  
Author(s):  
Saraswoti Aryal ◽  
Sanu Devi Joshi

Rauvolfia serpentina (L.) ex. Kurz is an important medicinal plant. Callus induction and regeneration was studied from stem explant of in-vitro grown plant of Rauvolfia serpentina(L.) Benth. ex Kurz (Apocynaceae) on Murashige Skoog (1962) medium supplemented with 1mg/l 2,4-Dichlorophenocy acetic acid (2,4-D) and 1mg/l Kinetin (Kn). Vigorous growth of callus occurs after 4 weeks of culture. Callus was sub-cultured on Murashige and Skoog (MS) medium supplemented with different concentration of 2, 4-D (0.5-3.0 mg/l) and 10% coconut milk. Regeneration of plantlets occurred on MS medium containing 3 mg/1 of 2, 4-D and 10% coconut milk. These plantlets were rooted on MS medium supplemented with 1 mg/l IAA .The regenerated plantlets were able to grow on soil after short period ofacclimatization. Key words: Explant; In-vitro culture; MS medium;  2, 4 Dichlorophenoxy acetic acid; Kinetin; Callus; Tissue culture; Coconut milk. Journal of Natural History Museum Vol. 24, 2009 Page: 82-88


2014 ◽  
Vol 49 (5) ◽  
pp. 595 ◽  
Author(s):  
Feng Huan ◽  
Yi Shuli ◽  
Xie Jiaheng ◽  
Lei Mengqi ◽  
Huang Xuan

Crop Science ◽  
1982 ◽  
Vol 22 (6) ◽  
pp. 1193-1197 ◽  
Author(s):  
J. V. Krans ◽  
V. T. Henning ◽  
K. C. Torres

Crop Science ◽  
1978 ◽  
Vol 18 (1) ◽  
pp. 157-159 ◽  
Author(s):  
B. V. Conger ◽  
J. V. Carabia

2021 ◽  
Vol 286 ◽  
pp. 110193
Author(s):  
Su-Fang Li ◽  
Tian-Wen Ye ◽  
Xin Xu ◽  
De-Yi Yuan ◽  
Shi-Xin Xiao

Molecules ◽  
2021 ◽  
Vol 26 (11) ◽  
pp. 3229
Author(s):  
Mat Yunus Najhah ◽  
Hawa Z. E. Jaafar ◽  
Jaafar Juju Nakasha ◽  
Mansor Hakiman

This study aims to investigate whether the in vitro-cultured L. pumila var. alata has higher antioxidant activity than its wild plant. An 8-week-old L. pumila var. alata nodal segment and leaf explants were cultured onto Murashige and Skoog (MS) medium supplemented with various cytokinins (zeatin, kinetin, and 6-benzylaminopurine (BAP)) for shoot multiplication and auxins (2,4-dichlorophenoxyacetic acid (2,4-D) and picloram) for callus induction, respectively. The results showed that 2 mg/L zeatin produced the optimal results for shoot and leaf development, and 0.5 mg/L 2,4-D produced the highest callus induction results (60%). After this, 0.5 mg/L 2,4-D was combined with 0.25 mg/L cytokinins and supplemented to the MS medium. The optimal results for callus induction (100%) with yellowish to greenish and compact texture were obtained using 0.5 mg/L 2,4-D combined with 0.25 mg/L zeatin. Leaves obtained from in vitro plantlets and wild plants as well as callus were extracted and analyzed for their antioxidant activities (DPPH and FRAP methods) and polyphenolic properties (total flavonoid and total phenolic content). When compared with leaf extracts of in vitro plantlets and wild plants of L. pumila var. alata, the callus extract displayed significantly higher antioxidant activities and total phenolic and flavonoid content. Hence, callus culture potentially can be adapted for antioxidant and polyphenolic production to satisfy pharmaceutical and nutraceutical needs while conserving wild L. pumila var. alata.


2021 ◽  
Author(s):  
Chenchen Wang ◽  
Haizhen Ma ◽  
Weiwei Zhu ◽  
Jiedao Zhang ◽  
Xiangyu Zhao ◽  
...  

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